The effect of exogenous gangliosides on matrix metalloproteinase secretion by human glioma cells in vitro

Maidment, Stephen L; Merzak, Abderrahim; Koochekpour, Shahriar; Rooprai, Harcharan K.; Rucklidge, Garry J.; Pilkington, G. J.

doi:10.1016/0959-8049(95)00659-1

Cited by 20 publications

(9 citation statements)

References 31 publications

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“…Earlier studies demonstrated that GM3 induced inhibition of cell migration and invasion in rat glioma cells and keratinocytederived SCC12 cells (21,34). On the other hand, other findings indicated that GM3 stimulated the proliferation, migration, and invasion of glioma and melanoma cells (35,36). In the present study, GM3 expression by transfection with the GM3 synthase gene led to the inhibition of invasion and migration in TNF-·-induced VSMC.…”

Section: Discussionsupporting

confidence: 47%

Suppression of vascular smooth muscle cell responses induced by TNF-α in GM3 synthase gene transfected cells

Moon¹

2010

Int J Mol Med

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Abstract. The natural accumulation of ganglioside GM3 (N-glycolylneuraminic acid) on atherosclerotic lesions is a common theory. The present study is the first to examine the effects of the GM3 synthase gene on the responses of vascular smooth muscle cells (VSMC) to tumor necrosis factor-· (TNF-·). We found that overexpression of the GM3 synthase gene inhibited DNA synthesis and ERK1/2 activity induced by TNF-· in VSMC, whereas the basal levels of DNA synthesis and ERK1/2 activity remained unchanged. In addition, GM3 synthase gene transfectants significantly reduced the migration and invasion of VSMC following TNF-· treatment, compared with empty vector transfectants. Furthermore, TNF-·-induced matrix metalloproteinase-9 (MMP-9) expression and promoter activity were also decreased in GM3 synthase gene transfectants. GM3 synthase gene expression markedly suppressed the TNF-·-stimulated transcriptional activity of activator protein-1 (AP-1) and nuclear factor-κB (NF-κB), which are the controlling factors of MMP-9 expression. Consistent with these results, the addition of anti-GM3 antibody into the GM3 synthase gene transfectants blocked inhibition of DNA synthesis, ERK1/2 activity, migration and invasion. Finally, GM3 synthase gene transfectants treated with anti-GM3 antibody reversed the suppression of MMP-9 expression by reducing AP-1 and NF-κB binding activity. These results suggest regulatory roles for the GM3 synthase gene in VSMC proliferation and migration during the formation of atherosclerotic lesions.

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Section: Discussionsupporting

confidence: 47%

Suppression of vascular smooth muscle cell responses induced by TNF-α in GM3 synthase gene transfected cells

Moon¹

2010

Int J Mol Med

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“…Researchers reported in 1996 that in 6 of 8 human glioma cell lines, gangliosides including GD1a upregulate MMP-9 secretion, while in two lines, downregulation was noted [20]. Whether gangliosides are involved in the regulation of MMP-9 transcription remains unknown.…”

Section: Discussionmentioning

confidence: 98%

Ganglioside GD1a Negatively Regulates Matrix Metalloproteinase-9 Expression in Mouse FBJ Cell Lines at the Transcriptional Level

Huang

Man

Wang

et al. 2007

Connective Tissue Research

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Mouse FBJ virus-induced osteosarcoma FBJ-S1 cells rich in GD1a are not readily metastatic, whereas FBJ-LL cells with low levels of GD1a are highly metastatic. GD1a was previously shown to suppress metastasis of mouse FBJ cells and to upregulate caveolin-1 and stromal interaction molecule 1 expression. The present study demonstrates that matrix metalloproteinase-9 (MMP-9) expression renders FBJ-LL cells invasive. MMP-9 is inversely regulated by GD1a, based upon four observations: MMP-9 mRNA content was 5 times higher in FBJ-LL cells than FBJ-S1 cells; a GD1a-re-expressing FBJ-LL cell variant produced through beta1,4GalNAcT-1 cDNA transfection expressed lower levels of MMP-9; exogenous addition of GD1a to FBJ-LL cells decreased MMP-9 production in a dose- and time-dependent manner; and treatment of GD1a-rich cells with D-PDMP or siRNA targeting St3gal2 decreased GD1a expression, but augmented MMP-9 expression. This is the first report demonstrating that GD1a negatively regulates expression of MMP-9 at the transcriptional level.

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“…The adhesive role of cell adhesion molecules may also be modulated by simple gangliosides on the cell surface [3,[12][13][14]. Indeed, such gangliosides are known to be associated with increased population doubling times [3], as well as stimulation of both proteases and vascular endothelial growth factor (VEGF) [15,16], each of which is a factor in tumor cell invasion.…”

Section: Mechanisms Of Migration and Invasionmentioning

confidence: 98%

Migration and invasion in brain neoplasms

Bolteus

Berens

Pilkington

2001

Curr Neurol Neurosci Rep

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Local invasion of the brain by neoplastic glial cells is a major obstacle to effective treatment of intrinsic brain tumors. Invasion is directly related to histologic malignancy, but occurs to some extent irrespective of tumor grade. Because the brain-to-tumor interface is not well demarcated, total surgical removal is rarely possible; moreover, as invading cells transiently arrest from cell division they are refractory to radiotherapeutic intervention. Invading cells may also be protected from the action of cytotoxic drugs by the presence of an intact blood-brain barrier. The invading cells, having migrated several millimeters or even centimeters from the main focus of the tumor, return to cycle phase under the control of some as yet unknown microenvironmental cue to form a recurrent tumor adjacent to the original site of presentation. Recent cellular and genetic information concerning factors underlying invasion may not only yield suitable targets for adaptation of existing therapies, but may also lead to novel approaches in glioma management.

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The effect of exogenous gangliosides on matrix metalloproteinase secretion by human glioma cells in vitro

Cited by 20 publications

References 31 publications

Suppression of vascular smooth muscle cell responses induced by TNF-α in GM3 synthase gene transfected cells

Suppression of vascular smooth muscle cell responses induced by TNF-α in GM3 synthase gene transfected cells

Ganglioside GD1a Negatively Regulates Matrix Metalloproteinase-9 Expression in Mouse FBJ Cell Lines at the Transcriptional Level

Migration and invasion in brain neoplasms

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