The effect of exogenous leptin on pancreatic
enzyme secretion in anaesthetized rats

Matyjek, R.; Woliński, Jarosław; Laubitz, Daniel; Kato, Seiyu; Zabielski, R.

doi:10.22358/jafs/67031/2005

Cited by 7 publications

(11 citation statements)

References 22 publications

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“…In a study reported by Matyjek et al (23), a significant reduction in the volume of secreted pancreatic juice was observed in fundectomized rats, compared to control animals. In addition, there was a decrease in the total protein content without changes in the level of trypsin in the pancreatic juice in fundectomized rats (23). In the present study, the pancreatic homogenates of ANT and Gx rats exhibited an increase in amylase activity by 30% and 47%, respectively.…”

Section: Resultssupporting

confidence: 49%

Effect of fundectomy, antrectomy and gastrectomy on pancreatic and brush border enzyme activity in rats

Kapica¹,

Puzio²

2018

Medycyna Weterynaryjna

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The aim of our study was to investigate the possible effects of the removal of different parts of the stomach (fundectomy, antrectomy, gastrectomy) on the total protein content and enzyme activity in the pancreas and the brush border of the intestinal mucosa. Twenty-four 2.5-month-old male Wistar rats were divided into four groups: sham-operated animals (SHO) and those subjected to gastrectomy (Gx), fundectomy (Fx), and antrectomy (ANT). After a six-week experiment, the rats were sacrificed, and blood was collected for further gastrin analysis in serum. Samples of the pancreas, duodenum, and jejunum (proximal part in 25% of length, middle part in 50% of length, and distal part in 75% of length) were collected to determine the total protein content and enzyme activity. The rats subjected to fundectomy, antrectomy and gastrectomy showed an increased total protein content and enzyme activity (amylase, trypsin) in pancreatic tissue. They exhibited an increase in the total protein content in the homogenates of the mucosa of the proximal, middle and distal jejunum, compared to the control, and a statistical increase in maltase activity. Compared with the control group, the rats subjected to Fx and ANT showed a decreased sucrase activity in the homogenates of the mucosa of the duodenum and of the proximal, middle and distal jejunum. In the gastrectomized rats, there was a statistically significant increase in the total protein content in the homogenates of the mucosa of the jejunum, compared to the control, while the activities of lactase and sucrase were decreased. There was a statistically significant increase in the gastrin level in all experimental groups (Fx, ANT, Gx). We suggest that surgical removal of a part of the stomach radically changes the level of hormones that determine many functions of the organism. Hormonal changes may have an impact on the pancreas and the activity of brush border enzymes. .

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Section: Resultssupporting

confidence: 49%

Effect of fundectomy, antrectomy and gastrectomy on pancreatic and brush border enzyme activity in rats

Kapica¹,

Puzio²

2018

Medycyna Weterynaryjna

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“…After thawing, 1 g of mucosa was homogenized with 5 ml of distilled water and centrifuged for 5 min at 1.000 × g at 4°C. The total protein content of supernatant was determined in accordance with the Lowry method modified to be performed in 96-microwell (Matyjek et al, 2004). Maltase and sucrase activities were determined (Dahlqvist, 1984) with minor modifications.…”

Section: Effect Of Feeding Transcarpathian Zeolite On Gastrointestinamentioning

confidence: 99%

Effect of Feeding Transcarpathian Zeolite on Gastrointestinal Morphology and Function in Broiler Chickens

Wawrzyniak

Kapica

Stępień–Pyśniak

et al. 2017

Rev. Bras. Cienc. Avic.

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Digestive enzyme, histology, small intestine, zeolite.Submitted: 10/August/2016 Approved: 27/February/2017 ABSTRACT Many kinds of feed additives can be used in chicken diets to stimulate intestinal morphological maturation and, consequently, intestinal function. The purpose of this study was to evaluate the effect of zeolite on broiler performance, gut morphology and gut digestive enzyme activity. The one-day old broiler chickens Ross 308 were assigned randomly into 3 groups with 30 birds per treatment. The 3 dietary treatments were: basal diet only (control group), basal diet + 2% zeolite, basal diet + 3% zeolite. Morphological and morphometric intestinal assessments were conducted using a light microscope. There was observed increase in villus height, villus perimeter, villus section area and crypt depth throughout duodenum and mid jejunum of the broilers fed zeolite. Supplementation especially with 2% zeolite was associated with greater villus heights, villus perimeter, villus section area and crypt depth in duodenum and ileum mucosa compared to the controls. The diet with 2% zeolite also contributed to significant increase proventriculus (pepsin) and pancreas (trypsin, lipase) activity compared to the controls The results suggest that dietary supplementation of zeolite increased feed consumption, intestinal morphology parameters and enzyme activity of gastrointestinal tract in broiler which improved growth performance.

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“…In cases of low-caloric diets and chronic exercise, ghrelin levels were increased; and in states of obesity or after glucose challenge, the concentrations of this peptide were reduced (Meier and Gressner, 2004;. Ghrelin stimulates the release of the growth hormone from the pituitary gland and increases appetite, food intake, body weight (BW), energy balance and especially fat tissue by decreasing fat oxidation in the liver (Matyjek et al, 2004;Barazzoni et al, 2005). The value of this peptide decreases over the period of childhood and puberty (Broglio et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Plasma concentration of leptin and ghrelin in Standardbred foals as related to the age, sex, exercise and training

Kędzierski¹,

Kapica²

2008

Animal

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The effect of acute exercise was studied in a group of 42 clinically healthy young Standardbred trotters. These trotters had been divided into four groups according to their age. Their ages were from 1.5 to 3 years. Three jugular venous blood samples were collected via venipuncture from each horse. These samples were collected while (1) at rest, (2) after the end of the exercise and (3) 30 min after the end of the exercise. Exercise showed a significant increase in plasma leptin concentration (3.8 6 0.31 at rest v. 4.3 6 0.37 just after exercise and 4.4 6 0.47 ng/ml after a 30-min rest; ANOVA P , 0.05). The difference between values obtained 30 min after exercise and at rest was significantly greater in 1.5-year-old horses than in those aged 2.5 years (11.3 6 0.43 v. 10.1 6 0.15 ng/ml; ANOVA P , 0.05). The mean plasma leptin concentration was higher in fillies than in colts (4.9 6 0.47 v. 3.5 6 0.36 ng/ml; ANOVA P , 0.05). A positive correlation between the plasma concentrations of leptin and triacylglycerides measured just after exercise was detected (r 5 0.65). The acute exercise significantly increased the plasma concentration of ghrelin that was measured just after exercise (1255 6 55.9 v. 1127 6 54.2 pg/ml; ANOVA P , 0.05). The exercise-induced age-related changes in the plasma ghrelin concentration were significantly lower in 2.5-year-old trotters than in 1.5-year olds. To sum up, the changes in plasma leptin and ghrelin concentrations during bouts of exertion tend to decrease with age and/or training of Standardbred foals.

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The effect of exogenous leptin on pancreatic enzyme secretion in anaesthetized rats

Cited by 7 publications

References 22 publications

Effect of fundectomy, antrectomy and gastrectomy on pancreatic and brush border enzyme activity in rats

Effect of fundectomy, antrectomy and gastrectomy on pancreatic and brush border enzyme activity in rats

Effect of Feeding Transcarpathian Zeolite on Gastrointestinal Morphology and Function in Broiler Chickens

Plasma concentration of leptin and ghrelin in Standardbred foals as related to the age, sex, exercise and training

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