The Effect of Exogenous Ribonucleic Acid on Alkaline Phosphatase Activity in the Ovariectomized Mouse Uterus

AM, Mansour

doi:10.1530/acta.0.0570465

Cited by 7 publications

(2 citation statements)

References 5 publications

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“…Such results suggest that hormonal stimulation induces the synthesis of RNA species which are responsible for subsequent uterine events such as general protein synthesis (Fencl & Villee, 1971), increase in enzymatic activity (Mansour, 1968) and epithelial hypertrophy (Segal, Davidson & Wada, 1965;Unjhem, Attramadal & Solna, 1968;Galand, Dupont-Mairesse, Leroy & Chrétien, 1971).…”

Section: Introductionmentioning

confidence: 99%

Induction of implantation in the rat by intraparametrial injection of uterine RNA from oestrogen-treated animals

Lejeune¹,

Puissant²,

Camus³

et al. 1982

Journal of Endocrinology

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Crude RNA preparations from uteri of oestradiol-treated rats induced the implantation of delayed blastocysts when injected into the parametrium of ovariectomized pregnant rats. Treatment of donor animals with labelled oestradiol showed that this effect could not be due to contamination of the RNA extracts by oestradiol. RNase digestion of these extracts suppressed their capacity to induce implantation. Purified poly (A)-rich RNA from oestrogen-treated uteri failed to elicit implantation although it was capable of increasing epithelial height when repeatedly injected into uterine horns of ovariectomized rats. These results suggest that uterine RNA synthesis might somehow mediate the effects of oestrogen in causing implantation and that RNAs other than messenger RNA might be involved.

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Section: Introductionmentioning

confidence: 99%

Induction of implantation in the rat by intraparametrial injection of uterine RNA from oestrogen-treated animals

Lejeune¹,

Puissant²,

Camus³

et al. 1982

Journal of Endocrinology

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“…They introduced a method to test the biological activity of such RNA preparations by observing morphologic changes in the uterine epithelium of rats in vivo. Subsequently, there have been other reports demonstrating that RNA preparations obtained from estrogen-stimulated uteri can induce hypertrophy of epithelial cells (4), can increase alkaline phosphatase activity (5), and can accelerate uterine protein synthesis (6). It should be noted, however, that uterine RNA can contain significant amounts of estrogen (7,8).…”

mentioning

confidence: 99%

Induction of Avidin Synthesis by RNA Obtained from Chick Oviduct

Tuohimaa

Segal

Koide

1972

Proc. Natl. Acad. Sci. U.S.A.

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RNA was extracted from oviduct, shell gland, and liver of chicks treated with diethylstilbestrol and a combination of diethylstilbestrol and progesterone. Preparations containing 500 or 2500 ug of RNA were put in the oviductal lumen of diethylstilbestrol-treated chicks for 24 hr, and the avidin content of the oviduct was determined by measurement of [14C]biotin binding.The results of the present study demonstrate that oviduct RNA from chicks treated with progesterone induces synthesis of avidin in chick oviduct. In contrast, liver or shell gland RNA from chicks treated with progesterone, and liver or oviduct RNA from chicks treated with diethylstilbestrol are incapable of inducing avidin synthesis. The inducing capacity of oviduct RNA is destroyed when it is treated with ribonuclease. It is hypothesized that progesterone induces the synthesis of new species of oviduct RNA, which serve as messenger(s) for avidin synthesis. This hypothesis is offered in consideration of the findings: (i) that the residual amount of progesterone present as a contaminant in oviduct RNA from chicks treated with progesterone is far too low to account for the induced avidin synthesis; (ii) that such oviduct RNA from chicks treated with progesterone induces a slight synthesis of avidin in chick shell gland, which ordinarily does not produce avidin in response to progesterone administration and; (iii) that such RNA added in vitro to minces of oviductal tissues incubated in biotin-free medium for 24 hr induces synthesis of avidin.One of the early effects of sex steroids on target organs of mammals is stimulation of nuclear RNA synthesis (1); this effect is blocked by the administration of actinomycin D (2).This phenomenon suggests that hormonally active steroids facilitate the transcription of genetic information. Segal et al. (3) presented evidence for an RNA fraction that could be administered to animals to mimic the events induced by the hormone. They introduced a method to test the biological activity of such RNA preparations by observing morphologic changes in the uterine epithelium of rats in vivo. Subsequently, there have been other reports demonstrating that RNA preparations obtained from estrogen-stimulated uteri can induce hypertrophy of epithelial cells (4), can increase alkaline phosphatase activity (5), and can accelerate uterine protein synthesis (6). It should be noted, however, that uterine RNA can contain significant amounts of estrogen (7,8). The presence of estrogen in the RNA fraction can interfere with the determination of the estrogen-mimetic activity of uterine RNA.Because of the presence of significant amounts of estrogen in rat uterine RNA it was deemed necessary to study another system. To establish whether hormone-induced RNA acts as a messenger or template for translation, it would be advantageous to analyze the synthesis of a specific protein induced by a hormone and to produce this induction by the application of the RNA fraction obtained from the target organ. Recently, two groups (9, 10) have rep...

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Hormonal Control of Metamorphosis

Tata¹

1970

Novartis Foundation Symposia

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The Effect of Exogenous Ribonucleic Acid on Alkaline Phosphatase Activity in the Ovariectomized Mouse Uterus

Cited by 7 publications

References 5 publications

Induction of implantation in the rat by intraparametrial injection of uterine RNA from oestrogen-treated animals

Induction of implantation in the rat by intraparametrial injection of uterine RNA from oestrogen-treated animals

Induction of Avidin Synthesis by RNA Obtained from Chick Oviduct

Hormonal Control of Metamorphosis

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