The effect of exposure to nanoparticles and nanomaterials on the mammalian epigenome

Sierra, Marta; Fernández, Adolfo; Torrecillas, Ramón; Fraga, Mario F.

doi:10.2147/ijn.s120104

Cited by 82 publications

(53 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the interaction of SNPs or silver ions with the other compounds and elements in human saliva should be better evaluated, 64 especially as the increased production of materials containing nanoparticles presents an important toxicological concern. 65,66 The results obtained in the present study showed that the materials modified with SNPs have a time-limited ion release. Similar results were obtained by Ferraris et al 56 Such materials will most probably exhibit antibacterial properties in the first weeks/months after implantation, until the silver concentration provides minimal bactericidal concentrations.…”

supporting

confidence: 51%

In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery

Pokrowiecki¹,

Zaręba²,

Szaraniec³

et al. 2017

IJN

View full text Add to dashboard Cite

The addition of an antibacterial agent to dental implants may provide the opportunity to decrease the percentage of implant failures due to peri-implantitis. For this purpose, in this study, the potential efficacy of nanosilver-doped titanium biomaterials was determined. Titanium disks were incorporated with silver nanoparticles over different time periods by Tollens reaction, which is considered to be an eco-friendly, cheap, and easy-to-perform method. The surface roughness, wettability, and silver release profile of each disc were measured. In addition, the antibacterial activity was also evaluated by using disk diffusion tests for bacteria frequently isolated from the peri-implant biofilm: Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, Streptococcus sanguis, Porphyromonas gingivalis, Staphylococcus aureus , and Escherichia coli . Cytotoxicity was evaluated in vitro in a natural human osteoblasts cell culture. The addition of nanosilver significantly increased the surface roughness and decreased the wettability in a dose-dependent manner. These surfaces were significantly toxic to all the tested bacteria following a 48-hour exposure, regardless of silver doping duration. A concentration of 0.05 ppm was sufficient to inhibit Gram-positive and Gram-negative species, with the latter being significantly more susceptible to silver ions. However, after the exposure of human osteoblasts to 0.1 ppm of silver ions, a significant decrease in cell viability was observed by using ToxiLight™ BioAssay Kit after 72 hours. Data from the present study indicated that the incorporation of nanosilver may influence the surface properties that are important in the implant healing process. The presence of nanosilver on the titanium provides an antibacterial activity related to the bacteria involved in peri-implantitis. Finally, the potential toxicological considerations of nanosilver should further be investigated, as both the antibacterial and cytotoxic properties may be observed at similar concentration ranges.

show abstract

supporting

confidence: 51%

In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery

Pokrowiecki¹,

Zaręba²,

Szaraniec³

et al. 2017

IJN

View full text Add to dashboard Cite

show abstract

“…high specific area, the use of nanomaterials increases every year in industrial and technological processes or in medical applications. Despite their exceptional qualities, their deleterious impact on the environment and health also gives rise to an increasing number of publications 1 – 4 . Among their numerous uses, nanoparticles and particularly metal-oxide nanoparticles, have often been used for their antibacterial properties, reviewed in 5 , 6 .…”

Section: Introductionmentioning

confidence: 99%

Impact of nanoparticles on the Bacillus subtilis (3610) competence

Eymard-Vernain

Luche

Rabilloud

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Due to the physicochemical properties of nanoparticles, the use of nanomaterials increases every year in industrial and medical processes. At the same time, the increasing number of bacteria becoming resistant to many antibiotics, mostly by a horizontal gene transfer process, is a major public health concern. We herein report, for the first time, the role of nanoparticles in the physiological induction of horizontal gene transfer in bacteria. Besides the most well-known impacts of nanoparticles on bacteria, i.e. death or oxidative stress, two nanoparticles, n-ZnO and n-TiO2, significantly and oppositely impact the transformation efficiency of Bacillus subtilis in biofilm growth conditions, by modification of the physiological processes involved in the induction of competence, the first step of transformation. This effect is the consequence of a physiological adaptation rather than a physical cell injury: two oligopeptide ABC transporters, OppABCDF and AppDFABC, are differentially expressed in response to nanoparticles. Interestingly, a third tested nanoparticle, n-Ag, has no significant effect on competence in our experimental conditions. Overall, these results show that nanoparticles, by altering bacterial physiology and especially competence, may have profound influences in unsuspected areas, such as the dissemination of antibiotic resistance in bacteria.

show abstract

“…Chronic and genotoxic effects of the nanomaterials have also been widely studied [41][42][43][44][45][46][47][48][49][50]. The Ames assay has been employed to observe Gene mutations in Chinese hamster lung (CHL/IU) cells upon exposure to fullerenes [51].…”

Section: Current Approaches For Toxicological Assessment Of Nanomatermentioning

confidence: 99%

Toxicological assessment of nanomaterials: the role of in vitro Raman microspectroscopic analysis

Efeoğlu¹,

Maher²,

Casey³

et al. 2017

Anal Bioanal Chem

View full text Add to dashboard Cite

The acceleration of nanomaterials research has brought about increased demands for rapid analysis of their bioactivity, in a multi-parametric fashion, to minimize the gap between potential applications and knowledge of their toxicological properties. The potential of Raman microspectroscopy for the analysis of biological systems with the aid of multivariate analysis techniques has been demonstrated. In this study, an overview of recent efforts towards establishing a 'label-free high content nanotoxicological assessment technique' using Raman microspectroscopy is presented. The current state of the art for cellular toxicity assessment and the potential of Raman microspectroscopy are discussed, and the spectral markers of the cellular toxic responses upon exposure to nanoparticles, changes on the identified spectral markers upon exposure to different nanoparticles, cell death mechanisms, and the effects of nanoparticles on different cell lines are summarized. Moreover, 3D toxicity plots of spectral markers, as a function of time and dose, are introduced as new methodology for toxicological analysis based on the intrinsic properties of the biomolecular changes, such as cytoplasmic RNA aberrations, protein and lipid damage associated with the toxic response. The 3D evolution of the spectral markers are correlated with the results obtained by commonly used cytotoxicity assays, and significant similarities are observed between band intensity and percentage viability obtained by the Alamar Blue assay, as an example. Therefore, the developed 3D plots can be used to identify toxicological properties of a nanomaterial and can potentially be used to predict toxicity, which can provide rapid advances in nanomedicine. Graphical Abstract Spectral markers of cytotoxicity as a function of time and dose.

show abstract

The effect of exposure to nanoparticles and nanomaterials on the mammalian epigenome

Cited by 82 publications

References 53 publications

In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery

In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery

Impact of nanoparticles on the Bacillus subtilis (3610) competence

Toxicological assessment of nanomaterials: the role of in vitro Raman microspectroscopic analysis

Contact Info

Product

Resources

About