The effect of external stimuli on the invertase adsorption capacity of poly(N-vinyl-2-pyrrolidone-co-itaconic acid) hydrogels

Tümtürk, Hayrettin; aykara, Tuncer; Kantoğlu, Ömer; Güven, Olgun

doi:10.1002/(sici)1522-9505(19991201)273:1<1::aid-apmc1>3.0.co;2-3

Cited by 7 publications

(4 citation statements)

References 25 publications

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“…This capacity to imbibe a solvent depends on the pH and ionic composition of the solution containing the hydrogel 3–16. These materials are of great interest because of their promising applications as sensors, separation membranes, adsorbents, and materials in medicine and pharmacy as drug‐delivery systems 4–13…”

Section: Introductionmentioning

confidence: 99%

Synthesis of N‐hydroxymethyl acrylamide with β‐methyl hydrogen itaconate and itaconic acid hydrogels: Effects of the pH, composition, and ionic strength on the swelling behavior

Jeria‐Orell

Pizarro

Marambio

et al. 2006

J of Applied Polymer Sci

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Copolymer gels were prepared by the radical copolymerization of N-hydroxymethyl acrylamide with itaconic acid and N-hydroxymethyl acrylamide with ␤-methyl hydrogen itaconate. The ratios of both monomers were varied from 25 to 75 mol %, and the effects of external stimuli such as the pH and ionic strength of the swelling media were investigated. The gels were pH-responsive, and an increase in the ionic strength of the solutions caused a decrease in the equilibrium degree of swelling of the copolymer gels. The monomer reactivity ratios were determined with the Kelen-Tü dö s method. Moreover, the copolymer composition had a significant effect on the equilibrium swelling behavior of the two copolymers investigated.

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Section: Introductionmentioning

confidence: 99%

Synthesis of N‐hydroxymethyl acrylamide with β‐methyl hydrogen itaconate and itaconic acid hydrogels: Effects of the pH, composition, and ionic strength on the swelling behavior

Jeria‐Orell

Pizarro

Marambio

et al. 2006

J of Applied Polymer Sci

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“…The copolymers of acrylamidemaleic anhydride were prepared by varying maleic anhydride content from 0.5 to 3%. The temperature, time, catalyst concentration, pH, and water content were kept constant 27–29. The results are tabulated in Table I.…”

Section: Resultsmentioning

confidence: 99%

Studies on usage of acrylamide copolymers for textile effluent treatment

Jain¹,

Jain²,

Jain³

2013

J of Applied Polymer Sci

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The copolymers of acrylamide-maleic anhydride and acrylamide-diallyl dimethyl ammonium chloride of different ionicities were used for the removal of color, solids, etc., from effluent generated from textile industry. The studies have been carried out to remove color generated in the effluent. To estimate dye content, we prepared standard curve using UV-vis spectrophotometer at different dye concentration following the Beer Lambert's Law. The copolymers of acrylamide-maleic anhydride and acrylamide-diallyl dimethyl ammonium chloride of known concentration were dosed in the known concentration of the dye. These polymers made flocks by adsorption with dyes and these colored flocks were filtered out. The intensity of the color of water went down on increasing the concentration of the polymers. The color of the textile effluent was removed to the extent of 97% using above mentioned polymers.

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“…In literature, there are certain values given for invertase loading. For example, 4.0-18.6 mg invertase binding per gram of polymeric supports and dry gels has been reported previously (Tümtürk et al 1999b(Tümtürk et al , 2000b.…”

Section: Enzyme Binding Efficiencymentioning

confidence: 90%

Covalent Immobilization of Invertase on Chemically Activated Poly (Styrene-2-Hydroxyethyl Methacrylate) Microbeads

Altinok

Aksoy

Tümtürk

et al. 2008

J Food Biochemistry

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A carrier for invertase enzyme was synthesized from styrene (S) and 2‐ hydroxyethyl methacrylate (HEMA) in the form of microbeads. These poly (styrene‐2‐hydroxyethyl methacrylate), P(S‐HEMA) microbeads were activated by epichlorohydrin (ECH) treatment for covalent immobilization. The free and immobilized invertase were assayed in the hydrolysis of sucrose to glucose, and the obtained results were compared. The optimum pH was 4.5 for free and 5.5 for immobilized invertase. The optimum temperature of invertase shifted from 45C to 55C upon immobilization. For free and immobilized enzymes, kinetic parameters were calculated as 4.1 × 10−3 mol L−1and 9.2 × 10−3 mol L−1for Km, and 6.6 × 10−2 mol L−1 min−1and 4.1 × 10−1 mol L−1 min−1for Vmax, respectively. After 1 month of storage at 4C, free enzyme retained 36% of its initial activity, while for the ECH‐activated P(S‐HEMA) immobilized enzyme, P(S‐HEMA)‐E, this value was observed as 67%. In repeated batch use, i.e., 20 times in 3 days, 78% retention of the initial activity was observed for P(S‐HEMA)‐E system. PRACTICAL APPLICATIONS Immobilization of enzymes are very important for many industrial applications, e.g., food, medicine, pharmacology, etc. Invertase converts sucrose to glucose and fructose, which have wide applications in food industry especially as sweeteners. Glucose–fructose mixture has much lower crystallinity compared to sucrose and therefore used in the production of noncrystallizing jams and creams. They are also used as liquid sweeteners. Immobilization enables repeated use, provides significant reduction in the operation costs, facilitates easy separation and speeds up recovery of enzyme and extends the stability of enzyme by protecting the active material from deactivation. Industrial application of immobilized invertase may decrease the production cost of glucose–fructose mixture because it could be used repeatedly for long periods. Although invertase is not a very expensive enzyme, the technique can also be applied to expensive ones for biotechnological productions.

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The effect of external stimuli on the invertase adsorption capacity of poly(N-vinyl-2-pyrrolidone-co-itaconic acid) hydrogels

Cited by 7 publications

References 25 publications

Synthesis of N‐hydroxymethyl acrylamide with β‐methyl hydrogen itaconate and itaconic acid hydrogels: Effects of the pH, composition, and ionic strength on the swelling behavior

Synthesis of N‐hydroxymethyl acrylamide with β‐methyl hydrogen itaconate and itaconic acid hydrogels: Effects of the pH, composition, and ionic strength on the swelling behavior

Studies on usage of acrylamide copolymers for textile effluent treatment

Covalent Immobilization of Invertase on Chemically Activated Poly (Styrene-2-Hydroxyethyl Methacrylate) Microbeads

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