The Effect of FATP1 on Adipocyte Differentiation in Qinchuan Beef Cattle

Abstract

FATP1 plays an important role in the regulation of fatty acid metabolism and lipid accumulation. In this study, we investigated the patterns of FATP1 expression in various tissues obtained from calf and adult Qinchuan cattle, and in differentiating adipocytes. Next, we investigated the effect of FATP1 expression on preadipocyte differentiation in Qinchuan cattle using overexpression and interference assays. We also identified the differentially expressed genes (DEGs) and pathways associated with FATP1 overexpression/interference. Our results reveal that FATP1 was broadly expressed in heart, kidney, muscle, small intestine, large intestine, and perirenal fat tissues. While FATP1 overexpression promoted preadipocyte differentiation, fat deposition, and the expression of several genes involved in fat metabolism, FATP1 interference had the opposite effects on adipocyte differentiation. Following FATP1 overexpression and FATP1 interference in adipocytes, RNA-seq analysis was performed to identify DEGs related to fat metabolism. The DEGs identified include SLPI, STC1, SEMA6A, TNFRSF19, SLN, PTGS2, ADCYP1, FADS2, and SCD. Pathway analysis revealed that the DEGs were enriched in the PPAR signaling pathway, AMPK signal pathway, and Insulin signaling pathway. Our results provide an in-depth understanding of the function and regulation mechanism of FAPT1 in fat metabolism.

Transcriptomics and Lipid Metabolomics Analysis of Subcutaneous, Visceral, and Abdominal Adipose Tissues of Beef Cattle

“…In this study, through the transcriptomics and metabolomics analysis of SAT, VAT, and AAT, 11 candidate genes were identified to affect fat deposition via their involvement in fatty acid synthesis, metabolism and oxidation, pyruvate metabolism, and carbohydrate synthesis and digestion (Figure 12), including fatty acid synthesis genes (ACACA, SCD, and ELOVL6), fatty acid oxidation genes (EHHADH and CPT2), ketoacid metabolism genes (ACADSB and OCXT1), glycolytic genes (PCK1 and PCK2), fatty acid transporter genes FABP7, and CHPT1. Additionally, it was further confirmed that PPAR signaling pathway was a pivotal pathway associated with fat deposition [41,42]. sic gene expression [40].…”

Effect of Cow-Calf Supplementation on Gene Expression, Processes, and Pathways Related to Adipogenesis and Lipogenesis in Longissimus thoracis Muscle of F1 Angus × Nellore Cattle at Weaning

“…In addition to stimulating lipogenesis, FATP1 ( SLC27A1 ) participates in the differentiation of pre-adipocytes regulated by PPAR [ 86 , 92 ]. During adipogenesis, FATP1 increases the release of triglycerides into plasma and promotes the uptake of fatty acids by pre-adipocytes [ 93 ]. Once inside the cells, fatty acids bind to the ligand-binding domain that modifies the structure of PPAR, forming a heterodimer with the retinoid X receptor (RXR) [ 56 ].…”

Effect of Cow-Calf Supplementation on Gene Expression, Processes and Pathways Related to Adipogenesis and Lipogenesis in Longissimus Thoracis Muscle of F1 Angus × Nellore Cattle at Weaning

“…In addition to stimulating lipogenesis, FATP1 (SLC27A1) participates in the differentiation of pre-adipocytes regulated by PPAR [90,96]. During adipogenesis, FATP1 increases the release of triglycerides into plasma and promotes the uptake of fatty acids by pre-adipocytes [97]. Once inside the cells, fatty acids bind to the ligand-binding domain that modifies the structure of PPAR, forming a heterodimer with the retinoid X receptor (RXR) [55].…”