The Effect of Feeding Behavior on Hypothalamus in Obese Type 2 Diabetic Rats with Glucagon-like Peptide-1 Receptor Agonist Intervention

Abstract

Objective: To investigate the utility of intravoxel incoherent motion-diffusion weighted imaging (IVIM-DWI) derived parameters in hypothalamus for monitoring the effect of Exendin-4 (Ex-4) intervention on the feeding behavior in obese diabetic rats within early feeding. Methods: 21 obese and 19 non-obese rats which were treated with streptozotocin injections were initially divided into an obese diabetes group (OD, n = 10), a non-obese diabetes group (D, n = 8), an obese group (O, n = 9) and a non-obese group (N, n = 9). Then, the rats in the 4 groups received subcutaneous injections of Ex-4, and feeding behavior was examined at 5, 35, 65, 95, and 125 min. The hypothalamic function was evaluated by IVIM-DWI. Finally, the relationship between the hypothalamic function and the amount of food intake was analyzed. Results: In comparison with the N group, the food intake significantly decreased in the O , OD, and D groups in response to Ex-4. Furthermore, a significant positive correlation was found between food intake and D values at different times from 5 to 125 min after Ex-4 intervention in all 4 groups. Conclusion: A direct correlation between the change of hypothalamic function and feeding behavior was detected in OD rats with Ex-4 intervention in the early feeding period. The hypothalamic D value derived from IVIM-DWI is promising to reflect the dynamic change of hypothalamic function due to intervention.

Intravoxel incoherent motion as a surrogate marker of perfused vascular density in rat brain

“…9 There is a significant variation in the reported IVIM measures across the limited existing literature on preclinical IVIM studies in rodent brain. 10,[41][42][43][44][45][46][47][48] We would like to highlight that the IVIM measures can be influenced by several factors, particularly in the context of preclinical imaging. These could include, but are not limited to, the magnetic field strength of the MRI scanner, the specifics of the IVIM acquisition scheme such as the number and distribution of b-values, repetition time, diffusion time, gradient duration, gradient separation, and the number and distribution of gradient directions.…”

“…There is a significant variation in the reported IVIM measures across the limited existing literature on preclinical IVIM studies in rodent brain 10,41–48 . We would like to highlight that the IVIM measures can be influenced by several factors, particularly in the context of preclinical imaging.…”

Altered Metabolic Phenotypes and Hypothalamic Neuronal Activity Triggered by Sodium-Glucose Cotransporter 2 Inhibition

“…Glucagon-like peptide-1 receptor agonists (GLP-1 RAs), antidiabetic medicines, are widely used to lower body weight due to their anorexigenic effect [ 27 ]. It has recently been highlighted that the clinical applications of SGLT-2 inhibitors in combination with GLP-1 RAs can lead to the concomitant synergic effect on the modulation of weight loss and hyperglycemia [ 28 ].…”

Potential Role of Hypothalamic and Plasma Ghrelin in the Feeding Behavior of Obese Type 2 Diabetic Rats with Intraventricular Glucagon-Like Peptide-1 Receptor Agonist Intervention

“…After 6 weeks of high-fat diet feeding, those rats with a weight gain 20% greater than the average body weight of the control group were classified as the obese group, and the others were classified as the non-obese group [25]. In all, 178 obese and 12 non-obese rats were obtained; 41 obese rats entered the diet-induced obesity (DIO) group, and the remaining 137 obese rats were further divided by randomly inducing diabetes.…”