The effect of fibronectin coating on endothelial cell kinetics in polytetrafluoroethylene grafts

Ramalanjaona, Georges; Kempczinski, Richard F.; Rosenman, John E.; Douville, E.Charles; Silberstein, Edward B.

doi:10.1016/0741-5214(86)90010-8

Cited by 87 publications

(20 citation statements)

References 15 publications

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“…This is in agreement with data reported previously. 5,8,[22][23][24][25][26] On seeded substrates, however, the number of platelets deposited on the substrates under stationary conditions slightly increased with endothelial cell density. Although similar numbers of ECs adhered to both uncoated and coated PS-CO 2 (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Coating of surfaces with von Willebrand factor, fibrinogen, or fibronectin increases adhesion of platelets, whereas coating with albumin or plasma decreases adhesion of platelets. [18][19][20][21][22] Seeding of ECs on artificial surfaces may result in decreased platelet deposition in dogs, 23 baboons, 24 and humans, 5,8,25 although at least one study failed to show this effect. 26 Decreased numbers of platelets adhering to endothelialized surfaces may be explained by the production of PGI 2 and nitric oxide by ECs.…”

Section: Introductionmentioning

confidence: 99%

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Blood compatibility of surfaces with immobilized albumin-heparin conjugate and effect of endothelial cell seeding on platelet adhesion

Bos

Scharenborg

Poot

et al. 1999

J. Biomed. Mater. Res.

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Endothelial cell (EC) seeding significantly improves the blood compatibility of artificial surfaces. Although a coating consisting of albumin and heparin (alb-hep) is a suitable substrate for seeded ECs, binding of ECs to the substrate further improves when small amounts of fibronectin are present in the alb-hep coating. Alb-hep conjugate was immobilized on carbon dioxide gas plasma-treated polystyrene (PS-CO(2)), thereby significantly increasing the recalcification time of blood plasma exposed to this surface. Furthermore, surface-immobilized alb-hep conjugate inhibited exogenous thrombin. Heparin activity was reduced by adding fibronectin on top of a monolayer of alb-hep conjugate, but not by simultaneous coating of fibronectin and alb-hep conjugate. Coating of PS-CO(2) with alb-hep conjugate significantly decreased contact activation (FXII activation). The number of platelets deposited from blood plasma on PS-CO(2) coated with alb-hep conjugate was twice as high as on PS-CO(2) coated with albumin. Addition of fibronectin to alb-hep conjugate-coated PS-CO(2) had no significant effect on the number of adhered platelets. Seeding of the substrates with ECs significantly reduced the number of adhered platelets under stationary conditions. Platelets deposited onto endothelialized surfaces were primarily found on endothelial cell edges, and sparingly on areas between ECs. In conclusion, alb-hep conjugate-coated surfaces display anticoagulant activity. ECs adhering to and proliferating on this coating significantly decrease the number of platelets which adhere to the surface. Therefore, alb-hep conjugate-coated surfaces form a suitable substrate for seeding of ECs in low density. Although application of fibronectin on top of the coating decreases the anticoagulant activity to some extent, it might be useful in view of the improved adherence of ECs to the coating.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Blood compatibility of surfaces with immobilized albumin-heparin conjugate and effect of endothelial cell seeding on platelet adhesion

Bos

Scharenborg

Poot

et al. 1999

J. Biomed. Mater. Res.

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“…In studies in which fibronectin glue was used to coat scaffolds, experimental animals were treated with anticoagulants to reduce acute graft complications such as thromboembolic events and subsequent graft failure. 33 The use of anticoagulants limits the clinical utility of such grafts, as these agents are often associated with significant morbidity and mortality secondary to the increased risk of hemorrhage and bleeding complications. Although biologic glues do have their associated limitations when used in static seeding, these glues may be applicable in increasing seeding efficiency when seeding is conducted by other means and uniform coverage may be attained.…”

Section: Cell-seeding Techniquesmentioning

confidence: 99%

Cell-Seeding Techniques in Vascular Tissue Engineering

Villalona

Udelsman

Duncan

et al. 2010

Tissue Engineering Part B: Reviews

184

136

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“…Under in vivo conditions, a significant number of seeded cells detach within a short time after restoration of blood flow in the treated graft although, compared to untreated grafts, there is less detachment. 12,13 Most of the ligand-based modifications are performed with single proteins such as Fn or Vn. 6,7,9,10,[14][15][16] A few studies have investigated a multiple ligand-based approach for the enhancement of EC adhesion and have shown that a combination of proteins support better EC adhesion than do single proteins.…”

Section: Introductionmentioning

confidence: 99%

Fibronectin and avidin-biotin as a heterogeneous ligand system for enhanced endothelial cell adhesion

Bhat

Truskey

Reichert

1998

J. Biomed. Mater. Res.

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A preadsorbed layer of "heterogeneous" integrin-dependent and -independent protein was used to enhance initial integrin-mediated endothelial cell attachment and spreading. Glass substrates were treated with fibronectin (Fn) and avidin coupled through adsorbed biotinylated bovine serum albumin (b-BSA). The slides then were seeded with biotinylated BAEC. Control "homogeneous" surfaces were slides adsorbed with either Fn or avidin coupled to b-BSA. The cells were incubated for 0.5 h in serum-containing media and exposed to a range of shear stresses in a laminar flow variable-height flow chamber. The critical shear stress to detach 50% of the seeded cells on the heterogeneous ligand surface was significantly greater than for either of the control homogeneous ligand systems (p < 0.001). Cellular spreading during the initial period of 0-2 h also was higher (p < 0.05) on the heterogeneous ligand-treated surface than on the surface of either of the homogeneous controls. The close contact area of the cell membrane with the substrate 1 h after seeding in serum-containing media was measured using TIRFM. Cells attached onto the heterogeneous ligand-treated surfaces had a significantly (p < 0.01) higher area of close contact with the substrate, which is consistent with a greater degree of attachment and spreading. The results indicate that the combination of integrin-dependent and -independent adhesion systems using heterogeneous ligands further enhances initial endothelial cell attachment and spreading.

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The effect of fibronectin coating on endothelial cell kinetics in polytetrafluoroethylene grafts

Cited by 87 publications

References 15 publications

Blood compatibility of surfaces with immobilized albumin-heparin conjugate and effect of endothelial cell seeding on platelet adhesion

Blood compatibility of surfaces with immobilized albumin-heparin conjugate and effect of endothelial cell seeding on platelet adhesion

Cell-Seeding Techniques in Vascular Tissue Engineering

Fibronectin and avidin-biotin as a heterogeneous ligand system for enhanced endothelial cell adhesion

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