The effect of fibronectin coating on endothelial cell kinetics in polytetrafluoroethylene grafts

Ramalanjaona, Georges; Kempczinski, Richard F.; Rosenman, John E.; Douville, E.Charles; Silberstein, Edward B.

doi:10.1067/mva.1986.avs0030264

Cited by 51 publications

(24 citation statements)

References 16 publications

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“…Our studies demonstrated optimal ASC attachment to this prepared scaffold after 2 h of incubation, with further cell spreading over 24 h. These results are in agreement with several laboratories that have attempted to overcome the limitations of cell seeding onto vascular grafts using a variety of techniques, including precoating with extracellular matrix proteins such as fibronectin. [39][40][41] The microenvironment of an EC in the circulation is under constant mechanical stress, including both fluid-induced cyclical shear stress as a product of the resistance encountered …”

Section: Discussionmentioning

confidence: 99%

Linear Shear Conditioning Improves Vascular Graft Retention of Adipose-Derived Stem Cells by Upregulation of the α₅β₁ Integrin

McIlhenny

Hager

Grabo

et al. 2010

Tissue Engineering Part A

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Use of adult adipose-derived stem cells (ASCs) as endothelial cell substitutes in vascular tissue engineering is attractive because of their availability. However, when seeded onto decellularized vascular scaffolding and exposed to physiological fluid shear force, ASCs are physically separated from the graft lumen. Herein we have investigated methods of increasing initial ASC attachment using luminal precoats and a novel protocol for the gradual introduction of shear stress to optimize ASC retention. Fibronectin coating of the graft lumen increased ASC attachment by nearly sixfold compared with negative controls. Gradual introduction of near physiological fluid shear stress using a novel bioreactor whereby flow rate was increased every second at a rate of 1.5 dynes/cm(2) per day resulted in complete luminal coverage compared with near complete cell loss following conventional daily abrupt increases. An upregulation of the alpha(5)beta(1) integrin was evinced following exposure to shear stress, which accounts for the observed increase in ASC retention on the graft lumen. These results indicated a novel method for seeding, conditioning, and retaining of adult stem cells on a decellularized vein scaffold within a high-shear stress microenvironment.

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Section: Discussionmentioning

confidence: 99%

Linear Shear Conditioning Improves Vascular Graft Retention of Adipose-Derived Stem Cells by Upregulation of the α₅β₁ Integrin

McIlhenny

Hager

Grabo

et al. 2010

Tissue Engineering Part A

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“…Specifically, reports have shown perturbed changes on filopodial protrusions for a characteristic nanoroughness behind 70 nm [61]. On the other hand, chemical modification of the biomaterial by coating the surface with ligand that binds to specific extracellular receptors has been largely proven to enhance seeding efficiencies [62–65], and recently in references [53,54]. In particular, in these papers, fibronecting or RGD-peptides coatings (which are recognized as a binding site by several members of the integrin family) have been shown to be efficient in cell adhesion and proliferation.…”

Section: Mechanisms For Cell-ecm and Artificial Substrate Interactionmentioning

confidence: 99%

Cell-Biomaterial Mechanical Interaction in the Framework of Tissue Engineering: Insights, Computational Modeling and Perspectives

Sanz-Herrera

Reina-Romo

2011

IJMS

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Tissue engineering is an emerging field of research which combines the use of cell-seeded biomaterials both in vitro and/or in vivo with the aim of promoting new tissue formation or regeneration. In this context, how cells colonize and interact with the biomaterial is critical in order to get a functional tissue engineering product. Cell-biomaterial interaction is referred to here as the phenomenon involved in adherent cells attachment to the biomaterial surface, and their related cell functions such as growth, differentiation, migration or apoptosis. This process is inherently complex in nature involving many physico-chemical events which take place at different scales ranging from molecular to cell body (organelle) levels. Moreover, it has been demonstrated that the mechanical environment at the cell-biomaterial location may play an important role in the subsequent cell function, which remains to be elucidated. In this paper, the state-of-the-art research in the physics and mechanics of cell-biomaterial interaction is reviewed with an emphasis on focal adhesions. The paper is focused on the different models developed at different scales available to simulate certain features of cell-biomaterial interaction. A proper understanding of cell-biomaterial interaction, as well as the development of predictive models in this sense, may add some light in tissue engineering and regenerative medicine fields.

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“…Fibronectin also supports the endothelial cell monolayer on expanded polytetrafluoroethylene (ePTFE) when exposed to varying levels of shear stress (2) and forms a stable complex with ePTFE graft material. There are specific receptors on the endothelial surface that interact with binding sites in the central portion of fibronectin molecule (2). Although several authors have studied the effect of fibronectin coating on endothelial cell binding to vascular prosthetic grafts (4)(5)(6), the optimum concentration of fibronectin and its optimum time of incubation with the graft have not been determined.…”

Section: Fibronectin Coating Of Expanded Polytetrafluoroethylenementioning

confidence: 99%

Fibronectin Coating of Expanded Polytetrafluoroethylene (ePTFE) Grafts and Its Role in Endothelial Seeding

et al. 1990

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Although fibronectin's role as a matrix to improve endothelial seeding has been demonstrated by other workers, the optimum concentration for use has never been described. Attachment of fibronectin to expanded polytetrafluoroethylene (ePTFE) was measured, using 125I‐radiolabeled protein, at different concentrations and for different time periods. The absolute amount of fibronectin bound to the graft increased with the concentrations used in coating (p < 0.001) and also with time (p < 0.01); e.g., at 50 μg/ml, 90 min of incubation produced a molecular attachment of 4.0 × 1011/cm2 of graft. However, its percentage attachment decreased with a rise in concentration (p < 0.001). After an initial loss of 22% in 30 min, the fibronectin‐graft bond was found to be stable when exposed to a shear stress produced by flow at 200 ml/min. No significant difference in the cell adherence could be found in grafts coated with fibronectin concentrations of 50, 150, and 250 μg/ml, although it was significantly less at 10 and 25 μg/ml (p < 0.05).

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The effect of fibronectin coating on endothelial cell kinetics in polytetrafluoroethylene grafts

Cited by 51 publications

References 16 publications

Linear Shear Conditioning Improves Vascular Graft Retention of Adipose-Derived Stem Cells by Upregulation of the α₅β₁ Integrin

Linear Shear Conditioning Improves Vascular Graft Retention of Adipose-Derived Stem Cells by Upregulation of the α₅β₁ Integrin

Cell-Biomaterial Mechanical Interaction in the Framework of Tissue Engineering: Insights, Computational Modeling and Perspectives

Fibronectin Coating of Expanded Polytetrafluoroethylene (ePTFE) Grafts and Its Role in Endothelial Seeding

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The effect of fibronectin coating on endothelial cell kinetics in polytetrafluoroethylene grafts

Cited by 51 publications

References 16 publications

Linear Shear Conditioning Improves Vascular Graft Retention of Adipose-Derived Stem Cells by Upregulation of the α5β1 Integrin

Linear Shear Conditioning Improves Vascular Graft Retention of Adipose-Derived Stem Cells by Upregulation of the α5β1 Integrin

Cell-Biomaterial Mechanical Interaction in the Framework of Tissue Engineering: Insights, Computational Modeling and Perspectives

Fibronectin Coating of Expanded Polytetrafluoroethylene (ePTFE) Grafts and Its Role in Endothelial Seeding

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Linear Shear Conditioning Improves Vascular Graft Retention of Adipose-Derived Stem Cells by Upregulation of the α₅β₁ Integrin

Linear Shear Conditioning Improves Vascular Graft Retention of Adipose-Derived Stem Cells by Upregulation of the α₅β₁ Integrin