The Effect of Flavonoids on Cancer and Normal Cell Lines invitro

Abstract

This study was aimed to know the inhibitory effect of the flavonoid extract of Silybum marianum seeds on two of the cancer cell lines , human brain cancinoma(AMGM), human cervix uteri epitheloid carcinoma (Hela ) and one normal cell line , Mouse embryonic fibroblast cell line (MEF). The concentration was (1000,500,250,125,62.5) µg/ml respectively with two exposure time (24 & 48) hours for the cancer cell lines and one exposure time (48) hr. for the normal one .The results showed that flavonoids extract has obvious inhibitory effect on (human brain carcinoma & Hela) with (P<0.05) significantly in high concentration (1000) µg/ml the cellular density (obsorption) of human brain carcinoma was (0.052) with inhibitory ratio (39%) compared with control , At 48 hours exposure the density was (0.051) with inhibitory ratio (94%) compared with control , whereas the density in Hela was (0.059, 0.06) with inhibitory ratio85.7% and 86% respectively at 24 and 48 hr. exposure .While the normal cell line showed no signification effect (P<0.05) for all brevious concentration except the high one (1000) µg/ml , whereas the density of MEF was (0.23), with inhibitory effect (34%) .The result of this study has indicated ahighest inhibitory activity of the cancer cell lines for both AMGM & Hela .