“…A less severe, more moderate injury can be induced whereby the tip of the notochord is used as the marker for transection, avoiding damage to the notochord and blood vessels (Figure 3) (Barros-Becker et al, 2017, Hoodless et al, 2016, Li et al, 2012, Lucas et al, 2013. A minor injury can be inflicted through making a small tissue nick in the tail fin (Elks et al, 2011;Paredes et al, 2019;Tauzin et al, 2014). We suggest that a moderate injury is employed when investigating NM toxicity in injured zebrafish to ensure the inflammatory response initiated is robust (i.e., can be detected) but avoids damage to critical structures such as the blood vessels or the notochord, as the latter may make quantification of the response more variable and challenging.…”