“…Dama et al [ 22 ] showed that the protein content of Pseudotolithus typus decreased significantly (P < 0.05) after 21 days of freezing at −20 °C. During freezing, the formation of macrocrystals perforates cell membranes, promoting protein exudation and denaturation [ 47 ]. In the fresh fish, lipid content is higher significantly (P < 0.05) in E. fimbriata than in other species.…”