The effect of gamma-ray and ethylene oxide sterilization on collagen-based wound-repair materials

Gorham, S. D.; Srivastava, S.; French, D. A.; Scott, Robert N.

doi:10.1007/bf00122976

Cited by 22 publications

(20 citation statements)

References 28 publications

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“…The reduced susceptibility of GTA-treated collagen matrices to enzymatic digestion is well documented. 26,27 Only 5% of collagen is solubilized by collagenase in collagen samples maximally crosslinked with GTA. This observation is in agreement with the results of Golomb et al in studies of collagenase digestibility of bovine pericardium crosslinked with GTA.…”

Section: Discussionmentioning

confidence: 99%

Dimethyl 3,3?-dithiobispropionimidate: A novel crosslinking reagent for collagen

Charulatha

Rajaram

2000

J. Biomed. Mater. Res.

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Crosslinking agents are used for improving the physical properties and durability of collagenous implants, glutaraldehyde (GTA) being the most widely used. Many of these reagents, including GTA, are known to be cytotoxic and to induce calcification. Hence, it is desirable to develop new crosslinking methods for collagen, so that biocompatibility and physical properties are improved. In the present study, dimethyl 3,3' -dithiobispropionimidate (DTBP) has been tried as a novel crosslinking reagent for collagen. Collagen purified from rat tail tendon has been crosslinked with DTBP and GTA. An increase of 22 degrees C in shrinkage temperature is observed for collagen treated with DTBP under optimal conditions. Crosslinking density determination shows that DTBP induces 10 crosslinks per mole, compared to 13 by GTA. While the tensile strength of GTA-treated samples is greater than those treated with DTBP, the latter shows more extensibility. In vitro degradation studies show that both GTA- and DTBP-treated samples are resistant to degradation by collagenase. The biocompatibility of crosslinked collagen samples studied by subcutaneous implantation in rats show that while both GTA- and DTBP-treated collagen do not degrade for up to 4 weeks, ultrastructural and histological studies indicate that DTBP collagen is far more biocompatible than GTA-treated matrices.

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Section: Discussionmentioning

confidence: 99%

Dimethyl 3,3?-dithiobispropionimidate: A novel crosslinking reagent for collagen

Charulatha

Rajaram

2000

J. Biomed. Mater. Res.

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“…Current sterilization methods, in particular gamma‐irradiation, are known to cause damage to the molecular structure of collagen, resulting in the breakdown of the α‐chains into smaller fragments 12. There is a dose‐dependent relationship, whereby as the radiation dosage increases the occurrence of fragmentation also increases 10, 13.…”

Section: Discussionmentioning

confidence: 99%

“…Having obtained evidence that our PEF method is effective in killing E. coli in a scaffold material, our aim here is to establish whether PEF treatment, as a potential sterilization method, is compatible with collagen‐based biomaterials. We specifically investigated the PEF treatment of collagen, because it is one of the most useful and widely used biomaterials25 due to its excellent biocompatibility and weak antigenicity 7, 12, 25, 26. Type I collagen was used in this study as it is the most abundant form of collagen (more than 90% of all fibrous mammalian protein is type I collagen27), and it is the type used for the majority of biomedical applications 7, 12…”

Section: Introductionmentioning

confidence: 99%

Pulsed electric field as a potential new method for microbial inactivation in scaffold materials for tissue engineering: The effect on collagen as a scaffold

Smith

Griffiths

MacGregor

et al. 2008

J Biomedical Materials Res

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Hybrid scaffolds for tissue engineering are becoming increasingly complex through incorporation of biologically active biomacromolecules. There is a need to develop a compatible sterilization method that is capable of killing microorganisms, without adversely affecting the labile scaffold biomaterials or biomacromolecular components. Pulsed electric field (PEF) treatment has been successful as a nonthermal microbial inactivation-pasteurization method within the food industry. We have previously demonstrated that PEF treatment inactivates E. coli seeded in collagen gels. Here, we show that PEF treatment does not affect the structural integrity of the collagen molecule or its adsorption to polystyrene and hydroxyapatite surfaces. Moreover, osteoblast cells cultured on PEF-treated collagen, which was coated onto two- and three-dimensional scaffolds, retained their normal morphology, growth rate, and functionality. PEF treatment, therefore, shows great potential to be used as a sterilization method for collagen-based biomaterials.

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“…Although it is an efficient method of sterilization, the process often results in chain scission and/or cross‐linking of biomolecules and synthetic polymers 8, 9. These effects change the mechanical properties and degradation rate of biomaterials,3–6, 8, 10–14 characteristics that are crucial to the success of the biomaterial post implantation. Production of degradation products may also present a toxicological risk 4.…”

Section: Introductionmentioning

confidence: 99%

“…However, it is mutagenic and carcinogenic,6, 14 and therefore, biomaterials require post‐treatment aeration, which can be for up to 2 weeks, to ensure that all residual EtO is removed 4. Moreover, when EtO is used for sterilization of collagen‐based biomaterials, it can react chemically with the amine groups of lysine or hydroxylysine,8, 12 resulting in a decrease in stability of the collagen molecule triple‐helix 8. Heat sterilization also clearly destroys the bioactivity of biomacromolecules.…”

Section: Introductionmentioning

confidence: 99%

Preparation and antidehydration of interpenetrating polymer network hydrogels based on 2‐hydroxyethyl methacrylate and N‐vinyl‐2‐pyrrolidone

Wang

Sun

2010

J of Applied Polymer Sci

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This work reports the preparation of 2-hydroxyethyl methacrylate (HEMA)/N-vinyl-2-pyrrolidone (NVP) interpenetrating polymer network (IPN) hydrogels by UV-initiated polymerization in the presence of free radical photoinitiator Darocur 1173 and cationic photoinitiator 4,4 0 -dimethyl diphenyl iodonium hexafluorophosphate. The polymerization mechanism was investigated by the formation of gel network. The structure and morphology of the HEMA/NVP IPN hydrogels were characterized by fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM). The results showed that the IPN gels exhibited homogeneous morphology. The dehydration rates of HEMA/NVP IPN hydrogels were examined by the gravimetric method. The results revealed that the hydrogels had a significant improvement of antidehydration ability in comparison with poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogel embedded physically with poly(Nvinyl-2-pyrrolidone)(PVP).

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The effect of gamma-ray and ethylene oxide sterilization on collagen-based wound-repair materials

Cited by 22 publications

References 28 publications

Dimethyl 3,3?-dithiobispropionimidate: A novel crosslinking reagent for collagen

Dimethyl 3,3?-dithiobispropionimidate: A novel crosslinking reagent for collagen

Pulsed electric field as a potential new method for microbial inactivation in scaffold materials for tissue engineering: The effect on collagen as a scaffold

Preparation and antidehydration of interpenetrating polymer network hydrogels based on 2‐hydroxyethyl methacrylate and N‐vinyl‐2‐pyrrolidone

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