The effect of gases in the intraperitoneal space on cytokine response and bacterial translocation in a rat model

Abstract

The results from this study suggest that the gas in the intraperitoneal space, but not the increased intraabdominal pressure, causes the alterations in host cytokine response and bacterial translocation. Carbon dioxide may play a primary role in the reduced immune response associated with laparoscopic surgery.

Effect of Abdominal Insufflation on Bacterial Growth in Experimental Peritonitis

“…These findings led us to believe that CO 2 insufflation might increase bacterial proliferation and peritoneal contamination in a setting of experimental peritonitis, possibly by inhibiting the mechanism of bacterial clearance. 6,13 The results of the present study show that pneumoperitoneum increases bacterial proliferation and peritoneal contamination in an experimental peritonitis model in rats. Increases in bacterial proliferation were significant in the CO 2 and N 2 O insufflation groups but insignificant in the group without pneumoperitoneum.…”

“…14 Carbon dioxide pneumoperitoneum probably stimulates bacterial growth or reduces immune responses in vivo. 6,13 Paksoy and associates 5 have shown that the quantitative bacteremia was significantly higher with E. coli injection in the laparotomy group than in the pneumoperitoneum group. Their study demonstrated that pneumoperitoneum impairs the clearance of bacteria from the peritoneal cavity in an experimental model of peritonitis.…”

“…Those investigators concluded that laparoscopic surgery is as safe an operation as open surgery in the treatment of acute peritonitis, and gasless laparoscopy may be used within the first 6-hour period of peritonitis. 16 Matsumoto et al 13 have shown that CO 2 in the intra-abdominal space reduces the systemic and local immune responses and induces less bacterial translocation than does air.…”

Immediate peritoneal response to bacterial contamination during laparoscopic surgery

“…Most observations have been made from analysis of the systemic response several hours after the procedure was performed. However, the initial response to tissue injury occurs early on a compartment level in the peritoneum [21].…”

Influence of O3/O2–pneumoperitoneum as an oxidative stressor on duration of anaesthesia, loss of different reflexes and cytokine mRNA expression

“…Because an early in uence of some anaesthetic drugs on the immune status has already been found (Bette et al 2004), we further evaluated if the effect of an oxidative stressor on depth and duration of anaesthesia is associated with changes in the anaesthesia-induced expression of cytokine mRNAs. Pure effects of different gases such as CO 2 , N 2 O, helium or air insuf ated into the abdomen have been described to in uence the intraperitonal immunity (Kamei et al 2001, Matsumoto et al 2001. Therefore, interactions between O 3 /O 2 gas mixture and anaesthetic agents on the local immune system in the abdomen are possible and are additionally evaluated in this study by characterization of splenic cytokine mRNA expression.…”