The effect of genistein for preventing granulose cell injury induced by cisplatin

Abstract

Genistein (GEN), the primary isoflavone in legumes, has a well known weak estrogenic effect by binding to estrogen receptors, and widely used for the treatment of ovary disease induced by chemotherapeutics, however, the details of the exact mechanisms was unclear so far, thus, the aim of our study was to find the effect on granulosa cells of ovary induced by cisplatin (CDDP) after using GEN treatment by (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) method and flow cytometry. The results demonstrated that CDDP could inhibit the proliferation of granulosa cells of ovary by affecting cell cycle S stage. Moreover, CDDP also could arrest cell cycle in G1-M stage, which would evidently increase the number of apoptotic cell. Genistein has the potential to prevent the damaging effect of CDDP and improve the differentiation and proliferations of the cells to make the blockage of the cell cycle disappear, which is related to the dose of GEN and time. The present study provides improvement in understanding the molecular pathogenic mechanism of premature ovarian failure (POF) induced by chemotherapeutics and development of GEN as effective treatment drugs.