The Effect of Ginger Hydroalcholic Extract on Rat Ileal Contraction in Vitro

Jalali-Nezhad, Ahmad Ali; Farajian-Mashhadi, Farzaneh; Komeili, Gholamreza; Barkhordari-Ahmadi, Farzaneh

doi:10.17795/zjrms-5984

Cited by 3 publications

(3 citation statements)

References 23 publications

(32 reference statements)

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“…Previous studies using isolated intestinal tract have reported that intrinsic pacemaker activity originates from ICCs of the Auerbach plexus, which are electrically coupled to smooth muscle cells [20,22]. ICCs play a major role in intestinal contraction without the involvement of motor neurons, hormones, or inflammatory mediators, and have been suggested to be modulated by several nutrients [23][24][25][26]. Another previous study reported an inhibitory action of caffeine on the smooth muscle contraction [27].…”

Section: Introductionmentioning

confidence: 99%

Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro

Yamashita¹

2018

IJNFS

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Thiamine and quinine are popular bitter substances and their physiological effects have been studied; however, their impact on digestion remains unknown. Here, the physiological effects of thiamine and quinine was investigated for in vitro contraction of mouse ileum. Acetylcholine stimulates autonomous contraction of mouse ileum in a dose-dependent manner. The effect of Acetylcholine for contraction of ileum was partly suppressed by the adrenaline administration. Upon simultaneous treatment of the ileum by acetylcholine, thiamine and quinine decreased the maximum contraction. The period till half maximum contraction was prolonged by the presence of thiamine and quinine but not by adrenaline. Because a physiological effect of thiamine and quinine was observed on acetylcholine-induced contraction of the ileum, the repertoire of human bitter taste receptors, TAS2R-1,-4,-7,-10,-14,-31,-39,-40,-43, and-46, were investigated to which thiamine and quinine may bind. These human bitter taste receptors were further analyzed among the database for mouse homologs using evolutionally conserved amino acid sequences. The only bitter receptor for both thiamine and quinine was TAS2R-39, the homology of TAS2R-139 to human TAS2R-39 was 74%. Importantly, the homology of mouse TAS2R-119 to human TAS2R-1 which interact with thiamine was 91%, and that of TAS2R-130 to human TAS2R-7 that interact with quinine was 81%. The present study indicated that thiamine and quinine changed the early phase of contraction of ileum in mice and suggested that TAS2R119 and TAS2R130 expressed in mouse enteroendocrine cells to modify the physiological effects of thiamine and quinine on the acetylcholine-induced contraction of the ileum.

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Section: Introductionmentioning

confidence: 99%

Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro

Yamashita¹

2018

IJNFS

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“…Recent studies on herbal medicines, such as Lizhong Tang and Dangkwisoo-san, reported that these modify the gastrointestinal motility or pacemaker activities of ICCs [30,31]. In addition, some herbal extracts of the roots of ginger and Liriope Platyphylla Wang et Tang have been reported to mediate the contraction of the ileal smooth muscle in rats [32,33]. Reportedly, naringenin, a citrus peel component, inhibits the pacemaking activity in ICCs [34].…”

Section: Discussionmentioning

confidence: 99%

Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine

Yamashita¹,

Shimamoto²,

Morita³

et al. 2018

JFNS

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Previously, the effects of several bitter substances have been investigated in the contraction of the murine jejunum and ileum, reporting that these independently suppress the rhythm generation of the interstitial cells of Cajal. Recently, it was reported that thiamin, which binds to a bitter taste receptor, modifies the early phase of the ileum contraction, whereas the physiological effects on the rhythm and amplitude of jejunum and ileum contractions remain unclear. In this study, it was investigated the physiological effects of thiamin and quinine on the in vitro contraction of the murine jejunum and ileum using mice for all experiments. the periodic contraction of the jejunum was observed before the administration of acetylcholine (Ach) and other substances, and the tonic amplitudes induced by the substances. These bitter substances variably affect the Achinduced rhythmic contraction of the jejunum and ileum in vitro. In addition, quinine hydrochloride (Qui) and thiamin hydrochloride (Thi) variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. Both Qui and Thi markedly increase the rhythmic contraction in the jejunum. Although Thi does not change the rhythmic contraction in the ileum, it gradually reduces the amplitude in the jejunum. Conversely, Qui gradually reduces the amplitude and almost inhibits the contraction in the jejunum. Furthermore, an antagonist of the adrenalin-beta3 receptor, SR59230A, enhances the Quiinduced inhibition of the contraction in the jejunum.

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“…It can help to treat gastrointestinal diseases, including nausea and vomiting from motion sickness, diarrhea, loss of appetite, and dry mouth [8,9].…”

Section: Introductionmentioning

confidence: 99%

Ginger-induced Ileal Motility is Modified by Stress: Sex Differences in Rats

Kimoto¹,

Zeredo²,

Ota³

et al. 2015

JFNS

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Abstract:Ginger is widely used as a spice and also an effective herbal medicine to treat gastrointestinal disorders. On the other hand, stress can induce various modulation of digestive motility. Here, we investigated ginger effects on stress-induced motility of the isolated ileum in male and female rats, in vitro. Rats (Wistar, SPF, 7-12 weeks of age, 148-393 g BW) were divided into Control (1G) and 3G groups. 3G stress (every day for 10 min) was loaded by centrifugal apparatus for 1, 3, 15 and 30 days. After the stress loading at each day, a 1 cm-long section of the ileum was isolated under barbiturate anesthesia and fixed to a Magnus-type chamber filled with Tyrode solution. Ileal movements were observed for 60 s following application of zingerone, which is also called vanillylacetone and a key component of the pungency of ginger (0.1-10 µM). Spontaneous motility movements with phasic and tonic patterns were observed in the ileum. The former was a peristalsis-like movement and the latter was a slow fluctuation of the baseline. Ginger induced enhanced effects on the rhythmic phasic motility in relation to amplitude. In the female, 3G gravity loading had no effects on the ginger-induced motility, however, suppressive effect of the ginger-induced phasic movements was clearly observed at day 15 in the male. The present study showed that gravity stress changed ginger-induced effects on phasic ileal motility in the male, but not in the female, indicating that sex differences were observed in the ginger effects modulated by stress loading.

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The Effect of Ginger Hydroalcholic Extract on Rat Ileal Contraction in Vitro

Abstract: Background: Ginger is a medicinal plant with wide use around the world which has several therapeutic implications especially in gastrointestinal discomfort such as dyspepsia, flatulence, colic, diarrhea, nausea and vomiting.

Cited by 3 publications

References 23 publications

Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum <i>in vitro</i>

Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum <i>in vitro</i>

Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum <i>In Vitro</i> by Thiamin and Quinine

Ginger-induced Ileal Motility is Modified by Stress: Sex Differences in Rats

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The Effect of Ginger Hydroalcholic Extract on Rat Ileal Contraction in Vitro

Abstract: Background: Ginger is a medicinal plant with wide use around the world which has several therapeutic implications especially in gastrointestinal discomfort such as dyspepsia, flatulence, colic, diarrhea, nausea and vomiting.

Cited by 3 publications

References 23 publications

Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum &lt;i&gt;in vitro&lt;/i&gt;

Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum &lt;i&gt;in vitro&lt;/i&gt;

Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum &lt;i&gt;In Vitro&lt;/i&gt; by Thiamin and Quinine

Ginger-induced Ileal Motility is Modified by Stress: Sex Differences in Rats

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Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum <i>in vitro</i>

Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum <i>in vitro</i>

Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum <i>In Vitro</i> by Thiamin and Quinine