The effect of glibenclamide on acetylcholine and sodium nitroprusside induced vasodilatation in human cutaneous microcirculation

Hojs, Nina; Štrucl, Martin; Cankar, Ksenija

doi:10.1111/j.1475-097x.2008.00833.x

Cited by 22 publications

(31 citation statements)

References 56 publications

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“…; Hojs et al . ) in young adults. Because prostacyclin is a major COX‐derived vasodilator prostanoid, it is plausible that a relationship would exist between COX‐dependent cutaneous vasodilatation and prostacyclin production.…”

Section: Discussionmentioning

confidence: 97%

Administration of prostacyclin modulates cutaneous blood flow but not sweating in young and older males: roles for nitric oxide and calcium‐activated potassium channels

Fujii

Notley

Kenny

2016

The Journal of Physiology

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Cyclooxygenase (COX) contributes to the regulation of cutaneous vasodilatation and sweating; however, the mechanism(s) underpinning this response remain unresolved. We hypothesized that prostacyclin (a COX-derived product) may directly mediate cutaneous vasodilatation and sweating through nitric oxide synthase (NOS) and calcium-activated potassium (KCa) channels in young adults. However, these responses would be diminished in older adults because ageing attenuates COX-dependent cutaneous vasodilatation and sweating. In young (25 ± 4 years) and older (60 ± 6 years) males (nine per group), cutaneous vascular conductance (CVC) and sweat rate were evaluated at four intradermal forearm skin sites: (i) control; (ii) 10 mm N -nitro-l-arginine (l-NNA), a non-specific NOS inhibitor; (iii) 50 mm tetraethylammonium (TEA), a non-specific KCa channel blocker; and (iv) 10 mm l-NNA + 50 mm TEA. All four sites were coadministered with prostacyclin in an incremental manner (0.04, 0.4, 4, 40 and 400 μm each for 25 min). Prostacyclin-induced increases in CVC were similar between groups (all concentrations, P > 0.05). l-NNA and TEA, as well as their combination, lowered CVC in young males at all prostacyclin concentrations (P ≤ 0.05), with the exception of l-NNA at 0.04 μm (P > 0.05). In older males, CVC during prostacyclin administration was not influenced by l-NNA (all concentrations), TEA (4-400 μm) or their combination (400 μm) (P > 0.05). No effect on sweat rate was observed in either group (all concentrations, P > 0.05). We conclude that, although prostacyclin does not mediate sweating, it modulates cutaneous vasodilatation to a similar extent in young and older males. Furthermore, although NOS and KCa channels contribute to the prostacyclin-induced cutaneous vasodilatation in young males, these contributions are diminished in older males.

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Section: Discussionmentioning

confidence: 97%

Administration of prostacyclin modulates cutaneous blood flow but not sweating in young and older males: roles for nitric oxide and calcium‐activated potassium channels

Fujii

Notley

Kenny

2016

The Journal of Physiology

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“…K ATP channels contribute to resting vascular tone and tissue blood flow in the coronary (128, 305, 354, 407–409, 641, 674, 995, 996, 1029, 1193, 1202, 1203, 1246, 1286, 1336, 1644), skin (2, 196, 613), splanchnic, and renal circulations (351, 614). In the heart it has been shown that the beating myocardium releases a K ATP channel agonist that activates these channels and dilates coronary arterioles (1376).…”

Section: Katp Channelsmentioning

confidence: 99%

Smooth Muscle Ion Channels and Regulation of Vascular Tone in Resistance Arteries and Arterioles

Tykocki

Boerman

Jackson

2017

Comprehensive Physiology

282

347

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Vascular tone of resistance arteries and arterioles determines peripheral vascular resistance, contributing to the regulation of blood pressure and blood flow to, and within the body’s tissues and organs. Ion channels in the plasma membrane and endoplasmic reticulum of vascular smooth muscle cells (SMCs) in these blood vessels importantly contribute to the regulation of intracellular Ca2+ concentration, the primary determinant of SMC contractile activity and vascular tone. Ion channels provide the main source of activator Ca2+ that determines vascular tone, and strongly contribute to setting and regulating membrane potential, which, in turn, regulates the open-state-probability of voltage gated Ca2+ channels (VGCCs), the primary source of Ca2+ in resistance artery and arteriolar SMCs. Ion channel function is also modulated by vasoconstrictors and vasodilators, contributing to all aspects of the regulation of vascular tone. This review will focus on the physiology of VGCCs, voltage-gated K+ (KV) channels, large-conductance Ca2+-activated K+ (BKCa) channels, strong-inward-rectifier K+ (KIR) channels, ATP-sensitive K+ (KATP) channels, ryanodine receptors (RyRs), inositol 1,4,5-trisphosphate receptors (IP3Rs), and a variety of transient receptor potential (TRP) channels that contribute to pressure-induced myogenic tone in resistance arteries and arterioles, the modulation of the function of these ion channels by vasoconstrictors and vasodilators, their role in the functional regulation of tissue blood flow and their dysfunction in diseases such as hypertension, obesity, and diabetes.

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“…; Orlov ) and K + channel inhibition typically causes an attenuated cutaneous vascular response (Hojs et al. ; Brunt and Minson ; Kutz et al. ), none have addressed their roles in cutaneous vasodilatation in humans during exercise.…”

Section: Introductionmentioning

confidence: 99%

The roles of the Na⁺ /K⁺ -ATPase, NKCC, and K⁺ channels in regulating local sweating and cutaneous blood flow during exercise in humans in vivo

et al. 2016

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Na+/K+‐ATPase has been shown to regulate the sweating and cutaneous vascular responses during exercise; however, similar studies have not been conducted to assess the roles of the Na‐K‐2Cl co‐transporter (NKCC) and K+ channels. Additionally, it remains to be determined if these mechanisms underpinning the heat loss responses differ with exercise intensity. Eleven young (24 ± 4 years) males performed three 30‐min semirecumbent cycling bouts at low (30% VO 2peak), moderate (50% VO 2peak), and high (70% VO 2peak) intensity, respectively, each separated by 20‐min recovery periods. Using intradermal microdialysis, four forearm skin sites were continuously perfused with either: (1) lactated Ringer solution (Control); (2) 6 mmol·L−1 ouabain (Na+/K+‐ATPase inhibitor); (3) 10 mmol·L−1 bumetanide (NKCC inhibitor); or (4) 50 mmol·L−1 BaCl2 (nonspecific K+ channel inhibitor); sites at which we assessed local sweat rate (LSR) and cutaneous vascular conductance (CVC). Inhibition of Na+/K+‐ATPase attenuated LSR compared to Control during the moderate and high‐intensity exercise bouts (both P ˂ 0.01), whereas attenuations with NKCC and K+ channel inhibition were only apparent during the high‐intensity exercise bout (both P ≤ 0.05). Na+/K+‐ATPase inhibition augmented CVC during all exercise intensities (all P ˂ 0.01), whereas CVC was greater with NKCC inhibition during the low‐intensity exercise only (P ˂ 0.01) and attenuated with K+ channel inhibition during the moderate and high‐intensity exercise conditions (both P ˂ 0.01). We show that Na+/K+‐ATPase, NKCC and K+ channels all contribute to the regulation of sweating and cutaneous blood flow but their influence is dependent on the intensity of dynamic exercise.

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The effect of glibenclamide on acetylcholine and sodium nitroprusside induced vasodilatation in human cutaneous microcirculation

Abstract: K(ATP) channels play an important role in human cutaneous vasodilatation induced by ACh and SNP.

Cited by 22 publications

References 56 publications

Administration of prostacyclin modulates cutaneous blood flow but not sweating in young and older males: roles for nitric oxide and calcium‐activated potassium channels

Administration of prostacyclin modulates cutaneous blood flow but not sweating in young and older males: roles for nitric oxide and calcium‐activated potassium channels

Smooth Muscle Ion Channels and Regulation of Vascular Tone in Resistance Arteries and Arterioles

The roles of the Na⁺ /K⁺ -ATPase, NKCC, and K⁺ channels in regulating local sweating and cutaneous blood flow during exercise in humans in vivo

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The effect of glibenclamide on acetylcholine and sodium nitroprusside induced vasodilatation in human cutaneous microcirculation

Abstract: K(ATP) channels play an important role in human cutaneous vasodilatation induced by ACh and SNP.

Cited by 22 publications

References 56 publications

Administration of prostacyclin modulates cutaneous blood flow but not sweating in young and older males: roles for nitric oxide and calcium‐activated potassium channels

Administration of prostacyclin modulates cutaneous blood flow but not sweating in young and older males: roles for nitric oxide and calcium‐activated potassium channels

Smooth Muscle Ion Channels and Regulation of Vascular Tone in Resistance Arteries and Arterioles

The roles of the Na+ /K+ -ATPase, NKCC, and K+ channels in regulating local sweating and cutaneous blood flow during exercise in humans in vivo

Contact Info

Product

Resources

About

The roles of the Na⁺ /K⁺ -ATPase, NKCC, and K⁺ channels in regulating local sweating and cutaneous blood flow during exercise in humans in vivo