The effect of glutamine on oxidative damage in an experimental abdominal compartment syndrome model in rats

Tıhan, Deniz; Erbil, Yeşim; Seven, Rıdvan; Arkaya, Selda Can; Türkoğlu, Ümit; Hepgül, Gülçin; Borucu, Ismail

doi:10.5505/tjtes.2011.73555

Cited by 22 publications

(21 citation statements)

References 46 publications

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“…It is reported that MPO increases during the ischemic period due to Pp and the reperfusion period after decompression [35]. When compared with the control group, MPO activity was higher in the Pp only group and it was thought that it increases as a result of I/R injury in our study.…”

Section: Resultssupporting

confidence: 50%

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The effects of Dexmedetomidine on oxidant - antioxidant systems in the experimental model of carbondioxide pneumoperitoneum

Taş¹

2014

Turk J Bioch

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Objective: The aim of the study was to investigate the changes of oxidative and anti-oxidative systems in the splanchnic area during carbon dioxide pneumoperitoneum and to determine whether the administration of dexmedetomidine has effects on these systems. Methods: Forty rats were randomized into four groups: Group I; Control, Group II; No pneumoperitoneum, Dexmedetomidine administration, Group III; Pneumoperitoneum, no Dexmedetomidine administration and Group IV; Pneumoperitoneum and Dexmedetomidine administration 30 minutes before insufflation. The rats were rested 30 minutes after desufflation and blood samples were obtained for; ischaemia modified albumin (IMA), myeloperoxidase (MPO), advanced oxidation protein products (AOPP), catalase (CAT), paraoxonase (PON1) and platelet-activating factor acetylhydrolase (PAF-AH) analyses. Results: When compared with the control group; the serum IMA levels significantly decreased in group II, and also increased in group III as compared to control (p<0.05). IMA levels were also significantly decreased in both groups II and IV as compared to group III (p<0.001). Serum MPO activity increased in group III as compared to control (p<0.05). Serum AOPP levels were significantly increased in group III as compared to group II (p<0.01) and decreased in group IV as compared to group III (p<0.01). Serum CAT activity was higher in group II than controls (p<0.05). Serum PON and plasma PAF-AH activities significantly decreased in grup III as compared to group II (p<0.05) and plasma PAF-AH activity were decreased in group III as compared to controls (p<0.05). Conclusion:In conclusion, administration of dexmedetomidine; prior to ischemia reperfusion injury caused by pneumoperitoneum; reduces the oxidative injury and increases the antioxidant activity in the acute period.

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Section: Resultssupporting

confidence: 50%

“…We believe that antioxidant effect of DEX derives from increasing CAT activity. Acute inflammatory response secondary to neutrophil activation in I/R injury releases reactive oxygen molecules and cytotoxic proteins such as MPO from neutrophils to the extracellular fluid [35]. An experimental study reported by Zhang et al…”

Section: Resultsmentioning

confidence: 99%

The effects of Dexmedetomidine on oxidant - antioxidant systems in the experimental model of carbondioxide pneumoperitoneum

Taş¹

2014

Turk J Bioch

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“…As a matter of fact, the antiapoptotic effects of Gln on T cells were investigated and it was found that Gln not only regulated intracellular oxidative balance but also precluded especially T cell apoptosis (Chang et al 2002). In accordance to this finding, a statistically significant increase in bcl-2 gene expression was thought to be the most important step of apoptosis preventing the effect of Gln in the period following its entry into the cell (Pithon-Curi et al 2003;Ran et al 2004;Tihan et al 2011). Again, it was determined that Gln administration to damaged tissues regulated release of free radicals (Kudsk 2002;Peng et al 2004).…”

Section: Discussionsupporting

confidence: 49%

“…Since Gln was a precursor of GSH, its supplementation in the clinical diet could be used to maintain high levels of GSH and to avoid oxidative stress damage (Amores-Sánchez and Medina 1999). Again, increased utilization of cellular Gln delayed and prevented neutrophil apoptosis (Tihan et al 2011). As a matter of fact, the antiapoptotic effects of Gln on T cells were investigated and it was found that Gln not only regulated intracellular oxidative balance but also precluded especially T cell apoptosis (Chang et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Ameliorative effect of supplementation with l-glutamine on oxidative stress, DNA damage, cell viability and hepatotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in rat hepatocyte cultures

et al. 2012

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The most potent of the dioxins, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is a persistent and ubiquitous environmental contaminant. And the health impact of exposure to TCDD is of great concern to the general public. Recent data indicate that Lglutamine (Gln) has antioxidant properties and may influence hepatotoxicity. The objective of the present study was undertaken to explore the effectiveness of Gln in alleviating the hepatotoxicity of TCDD on primary cultured rat hepatocytes. Gln (0.5, 1 and 2 mM) was added to cultures alone or simultaneously with TCDD (0.005 and 0.01 mM). The hepatocytes were treated with TCDD and Gln for 48 h. Then cell viability was detected by [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) assay and lactate dehydrogenase (LDH) release, while total antioxidant capacity (TAC), total glutathione (TGSH) and total oxidative stress (TOS) levels were determined to evaluate the oxidative injury. The DNA damage was also analyzed by liver micronucleus assay (MN) and 8-oxo-2-deoxyguanosine (8-OH-dG). The results of MTT and LDH assays showed that TCDD decreased cell viability but not L-glutamine. TCDD also increased TOS level in rat hepatocytes and significantly decreased TAC and TGSH levels. On the basis of increasing doses, the dioxin in a dosedependent manner caused significant increases of micronucleated hepatocytes (MNHEPs) and 8-OH-dG as compared to control culture. Whereas, in cultures exposured with Gln alone, TOS levels were not changed and TAC and TGSH together were significantly increased in dose-dependent fashion. The presence of Gln with TCDD modulated the hepatotoxic effects of TCDD on primary hepatocytes cultures. Noteworthy, Gln has a protective effect against TCDD-mediated DNA damages. As conclusion, we reported here an increased potential therapeutic significance of L-glutamine in TCDD-mediated hepatic injury for the first time.

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“…La actividad de MPO se calculó con el empleo del cociente de extinción de o-dianisidina. Los resultados se expresaron en unidades por gramo de tejido húmedo (U/g) 24 .…”

Section: Determinación De La Actividad De Mieloperoxidasaunclassified

Efectos protectores del aceite de Nigella sativa en la lesión pulmonar inducida por hiperoxia

Tayman¹,

Çekmez²,

Kafa

et al. 2013

Archivos de Bronconeumología

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The effect of glutamine on oxidative damage in an experimental abdominal compartment syndrome model in rats

Cited by 22 publications

References 46 publications

The effects of Dexmedetomidine on oxidant - antioxidant systems in the experimental model of carbondioxide pneumoperitoneum

The effects of Dexmedetomidine on oxidant - antioxidant systems in the experimental model of carbondioxide pneumoperitoneum

Ameliorative effect of supplementation with l-glutamine on oxidative stress, DNA damage, cell viability and hepatotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in rat hepatocyte cultures

Efectos protectores del aceite de Nigella sativa en la lesión pulmonar inducida por hiperoxia

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