The Effect of Glutathione Addition in Diluent Semen on Ram Spermatozoa Quality

Abstract

Cryopreservation of ram sperm reduces sperm motility and breaks sperm membrane integrity due to free radicals developed by reactive oxygen species (ROS). Moreover, it provokes cryodamage and deteriorates sperm quality. This study investigated the effect of glutathione addition as an antioxidant agent on the quality of local ram spermatozoa. The impacts of glutathione additions were observed in five different concentrations (control, 0.5 mM, 1 mM, and 1.5 mM or treatment P0-P4); the extender used to dilute the semen of two rams was andromed diluents. This research examined factors including the quality of fresh sperm, sperm motility, and membrane integrity after chilled. The data in this research was analyzed using one-way ANOVA to determine the effect of glutathione addition between the control and treatment groups. The outcomes demonstrated that adding glutathione significantly (p<0.05) enhanced motility and membrane integrity after stored in cold storage for 48 hours. In comparison with the control, the P1 concentration generated the best results in terms of ensuring the integrity of ram spermatozoa after processing. Glutathione concentration of 0.1mM (P1) showed the best motility and membrane integrity at 0 hours and 48 hours cold storage (82.50 ± 0.92 versus 65.35 ± 1.80% and 87.50 ± 0.50 versus 68.75 ± 1, 83%. In conclusion, the addition of glutathione affected the quality of local sheep spermatozoa after cooling. We obtain the best results of membrane motility and integrity at the glutathione level of 0.1 nM.

Effects of Adding Glutathione to AndroMed Diluent on Intact Plasma and Acrosome Membranes, and Progressive Motility of Cattle Spermatozoa During Freezing Processes

“…The main challenge encountered during semen freezing was not only cold shock but also the exposure of spermatozoa to free radical attacks such as hydroxyl and singlet oxygen (Park and Yu, 2017;Angrimani et al, 2018). These highly reactive radicals can induce lipid peroxidation on plasma and acrosome membranes (Bansal and Bilaspuri, 2011;Syafitri et al, 2022). The spermatozoa plasma membrane consists of double lipids containing unsaturated fatty acids which are very susceptible to peroxidation (Douard et al, 2003).…”

“…Research related to antioxidants as cryoprotectants has reported success in reducing oxidative damage due to reactive oxygen species (Zulaikhah, 2017;Liu et al, 2021). Glutathione is a significant antioxidant that neutralizes free radicals for cryopreserved in dogs (Angrimani et al, 2018), Indian red jungle fowl (Ansari et al, 2021), Mithun cattle (Perumal et al, 2021), goat (Zou et al, 2021) and sheep (Syafitri et al, 2022). It is a naturally occurring substance present in the body since birth and is found both within and outside cells (Zou et al, 2021).…”

Comparison Enzymatic and Non-Enzymatic Antioxidant in Sperm Quality of Garut Ram Chilled Semen to Enhance Rural Livestock Commodity

“…Antioxidant compounds are usually added to semen diluents to minimize the damage caused by free radicals. These antioxidant compounds interact with free radicals, thereby minimizing damage to the spermatozoa plasma membrane (Syafitri et al, 2022). Spermatozoa damage caused by oxidation is mitigated or eliminated when antioxidants are provided (Samoylenko et al, 2013).…”