The Effect of Glyphosate on the Reproduction of Estuarine Crabs: Neohelice granulata as a Study Model

Abstract

This review summarizes the bulk of evidence about the effect of glyphosate, both technical and formulated, on the ovarian maturation of Neohelice granulata female crabs, as well as the effects of glyphosate on sperm production in males of the same species. After long-term in vivo assays, made during the 3-month pre-reproductive period of this species, both formulated and technical glyphosate were able to produce a significant incidence of oocyte reabsorption in the ovary, together with a concomitant decreased of vitellogenin content, at concentrations ranging from 0.2 to 1 mg/L. Despite this, after 32-day in vivo assays, glyphosate stimulated oocyte growth, in terms of a higher percentage of vitellogenic oocytes, suggesting that glyphosate could be acting as an endocrine disruptor. In vitro assays made with isolated ovarian pieces showed a decrease of vitellogenin content, in correlation with lower protein synthesis, although some advance in maturation was observed in the histological analysis. In male crabs exposed in vivo to both technical and formulated glyphosate at 1 mg/L, several reproductive imbalances were noted, such as a significant decrease of the sperm count, abnormal spermatophores, and possible disrupting effects of glyphosate on the androgenic gland.

Ovarian development of Xiphopenaeus kroyeri (Crustacea: Penaeidae) from Espírito Santo, southeastern Brazil

“…Oocytes in atresia are the result of resorption of mature oocytes that were not released during spawning (Peixoto et al, 2005). Although atretic oocytes were only observed at the spawning stage in the present study, oocyte resorption can also be triggered by environmental impacts, such as pesticide contamination, for example (Rodríguez et al, 2021); and they should only be used as the main classification characteristic when one has full knowledge of the local environmental parameters. In this case, we also used the differentiation of the stages according to the thickness of the gonadal wall, which tends to become thicker, and looser as new reproductive events occur.…”

Marine invertebrates and sound

“…The Mar Chiquita coastal lagoon is a wetland located in the Buenos Aires Province, Argentina (37°40′ S, 57°23′ W) declared as a Man and the Biosphere Reserve (MAB) by UNESCO, conforming to the UNESCO World Network of Biosphere Reserves. In this lagoon, N. granulata is a dominant species used as a model study given the great diversity of publications conducted on several topics of its physiology, ecology and behavior (see Luppi and Rodriguez, 2020;Rodriguez and Luppi, 2020). Moreover, this crab is considered a key species and an ecosystem engineer because of its burrowing activity that regulates the estuarine ecosystem functioning (Gutieŕrez et al, 2006).…”

Glyphosate Interference in Follicular Organization in the Wall Lizard Podarcis siculus

“…Glyphosate is in fact highly soluble in water [ 18 ]. In both invertebrates and vertebrates, in vivo exposure to Gly or GBH induces damage similar to that observed in mammals, especially in the reproductive system [ 19 , 20 ]. In zebrafish, Gly reduced fecundity and increased early-stage embryo mortalities and premature hatching.…”