The effect of gold salt on lysosomal enzymes of the peritoneal macrophage

Persellin, Robert H.; Ziff, Morris

doi:10.1002/art.1780090107

Cited by 198 publications

(72 citation statements)

References 27 publications

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“…So far, it has been suggested that gold can inhibit the activity of lysosomal enzymes in phagocytic cells. [66] Knowledge about the influence of gold ions on cells of the immune system is still modest. It was recognised that gold ions are powerful inhibitors of macrophages and polymorphonuclear leucocytes.…”

Section: Discussionmentioning

confidence: 99%

Gold nanoparticles and ions – friends or foes? As they are seen by human cells U-937 and HL-60

Barbasz

Oćwieja

2015

Journal of Experimental Nanoscience

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Gold nanoparticles (AuN) are one of the most investigated nanomaterials, finding numerous applications from medicine to industry. AuN were obtained by reduction of gold salts using tannic acid as a reducing agent. To detach the impact of AuN onto cells of two lines human monocytic cells (U-937) and human promyelocytic leukaemia cells (HL-60), the effects of postreaction residues (of effluent from sol cleaning) and gold ions were also examined. It was demonstrated that resistance of cells to the toxic action of AuN is dependent not only on incubation time and dosage, but also on stages of cell differentiation. It was found that after incubation of promonocytes U-937 with 25 ppm AuN, the cell viability decreased by 25% and of macrophages by 50%. Differentiated cells U-937 showed a significantly lower resistance than the not-differentiated cells. For HL-60 cells, regardless of differentiation, cell viability decreased by approximately 20% after treatment with 25 ppm AuN sol. It was noticed that U-937 exhibited higher vulnerability to AuN than HL-60 cells. It was proved that AuN induced over a 15-fold increase in nitric oxide and a decrease of intracellular glutathione levels indicating the inflammatory response of cells. Even though gold ions did not show a significant effect on cell viability, they caused fivefold increase of nitric oxide level. The results show a higher cytotoxicity of AuN than gold ions. An overall picture of the interaction of AuN with human cells of first defence line was obtained. The results indicate that AuN may cause changes in the response of phagocytes in inflammatory conditions.

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Section: Discussionmentioning

confidence: 99%

Gold nanoparticles and ions – friends or foes? As they are seen by human cells U-937 and HL-60

Barbasz

Oćwieja

2015

Journal of Experimental Nanoscience

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“…The higher concentration of gold in Kupffer cells, presumably in lysosomes, together with the higher hydrolytic enzyme concentration, suggests that one action of gold may be enzyme inhibition in reticuloendothelial cells. Earlier studies (6) showed that the critical gold level required for this action in vitro was considerably higher (250 pg/ml) than that attained in serum during standard chrysotherapy (3-5 pg/ml), although some tissues (lymph nodes) achieve this concentration after protracted treatment (7). In the current experiments the concentration of radiogold in lysosomes was 4.5 times Triton X-100 is a polydisperse preparation ofp-toctylphenoxy-polyethoxyethanols consisting of oxyethylene chain lengths averaging 9-10 oxyethylene units (39).…”

Section: Resultsmentioning

confidence: 98%

Intracellular distribution of radiogold. Localization to large granule membranes

Penneys¹,

McCreary²,

Gottlieb³

1976

Arthritis & Rheumatism

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The distribution of gold in Kupffer cells and in subcellular fractions of rat liver was studied at intervals following intraperitoneal injection of lssAu sodium thiomalate (Myochrysine). Kupffer cells, isolated by digestion of whole liver with Pronase, had radioactive gold counts per milligram of protein that were twice the counts in the digested liver supernatant. After fractionation of liver cells by differential centrifugation, radiogold was found predominantly in the nuclear, mitochondrial, and lysosomal fractions. When the distribution of isotope was related to the protein content, the highest gold concentration was found in the lysosomal fraction, where it was 28 times that in the soluble fraction. Most radiogold was nondialyzable, probably a result of binding to larger intracellular compounds. Approximately 95% of the nondialyzable gold was in the organelle membrane or attached to membrane-adsorbed material, as determined by treatment of dialyzed mitochondria1 and lysosomal fractions with Triton X-100. These data suggest that the intracellular locus of gold action may reside in organelle membranes.from the

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“…Lysosomes have the ability to concentrate many substances which are fed to cells. This was shown dramatically by Allison and Young(1969), who found that they concentrated acridine orange; Fell and Dingle (1969) found they took up sucrose, which they were unable to digest; Persellin and Ziff (1966), Ennis, Granda, and Posner (1967), and Lorber, Cutler, and Chang (1968) showed that lysosomes of phagocytic cells concentrated gold salts which act as inhibitors of lysosomal hydrolases.…”

Section: Contents Of Lysosomesmentioning

confidence: 95%

Lysosomal enzymes and inflammation with particular reference to rheumatoid diseases

Chayen¹,

Bitensky²

1972

Plastic and Reconstructive Surgery

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The effect of gold salt on lysosomal enzymes of the peritoneal macrophage

Cited by 198 publications

References 27 publications

Gold nanoparticles and ions – friends or foes? As they are seen by human cells U-937 and HL-60

Gold nanoparticles and ions – friends or foes? As they are seen by human cells U-937 and HL-60

Intracellular distribution of radiogold. Localization to large granule membranes

Lysosomal enzymes and inflammation with particular reference to rheumatoid diseases

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