The Effect of Granulocyte-colony Stimulating Factor on Rotator Cuff Healing After Injury and Repair

Ross, David L.; Maerz, Tristan; Kurdziel, Michael D.; Hein, Joel; Doshi, Shashin; Bedi, Asheesh; Anderson, Kyle; Baker, Kevin C.

doi:10.1007/s11999-015-4218-9

Cited by 26 publications

(20 citation statements)

References 55 publications

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“…The average weight of the animals was 170–230 g. The animals were housed in wire mesh cages at room temperature with 12:12-h light-dark cycles and were maintained on standard rat chow and tap water. Animals were randomly divided into seven groups (12 animals each): group 1: a negative control; group 2: induced endometrial fibrosis (pathological control); group 3: induced endometrial fibrosis that received human mesenchymal stem cells (hMSCs) (2 × 10 6 cells/ml intraperitoneally/week) [ 8 ]; group 4: induced endometrial fibrosis that received 0.1 mg/kg daily oral estrogen [ 8 ], group 5: induced endometrial fibrosis that received 2 × 10 6 hMSCs + 0.1 mg/kg and daily oral estrogen; group 6: induced endometrial fibrosis that received neupogen (300 µg/ml IV injection in tail vein, three times/week) [ 9 ], and group 7: induced endometrial fibrosis that received 2 × 10 6 hMSCs + 300 µg/ml neupogen.…”

Section: Methodsmentioning

confidence: 99%

Neupogen and mesenchymal stem cells are the novel therapeutic agents in regeneration of induced endometrial fibrosis in experimental rats

et al. 2017

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Endometrial fibrosis is the presence of intrauterine adhesions (IUAs) after any uterine surgery or curettage and it results in infertility and recurrent pregnancy loss. We evaluated the role of human mesenchymal stem cells (hMSCs) as a therapeutic agent of endometrial fibrosis. We also compared the effect of MSCs with the effect of estrogen and neupogen either each alone or as a combined therapy with MSCs. This experimental study was performed on 84 albino rats which were divided into seven groups (n=12 rats/group) as follows, group1: normal control rats, group 2: induced fibrosis, group 3: induced fibrosis that received oral estrogen, group 4: induced fibrosis that received hMSCs, group 5: induced fibrosis that received hMSCs and estrogen, group 6: induced fibrosis that received neupogen, and group 7: induced fibrosis that received hMSCs and neupogen. The extent of fibrosis, vascularization, and inflammation were evaluated by; qRT-PCR for interleukin 1 (IL-1), interleukin 6 (IL-6), TNF, vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β), and RUNX; ELISA for connective tissue growth factor (CTGF); Western blotting for collagen-I; immunohistochemistry examination for VEGF and RUNX-2; and histopathological assessment. In therapeutic groups either by hMSCs alone or combined with estrogen or neupogen; fibrosis and inflammation (IL-1, IL-6, TNF, TGF-β, RUNX, CTGF, and collagen-I) were significantly decreased but vascularization (VEGF) was significantly increased (P<0.05) compared with induced fibrosis group. The most significant result was obtained in fibrosis that received combined therapy of hMSCs and neupogen (P=0.000). Stem cells and neupogen are a highly effective alternative regenerative agents in endometrial fibrosis.

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Section: Methodsmentioning

confidence: 99%

Neupogen and mesenchymal stem cells are the novel therapeutic agents in regeneration of induced endometrial fibrosis in experimental rats

et al. 2017

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“…After acute traumatic brain injury, G-CSF application reduced bacteremia, although overall survival was not improved [ 149 ]. However, contradictory effects were reported concerning local healing: In a rodent model of full-thickness supraspinatus tendon defects, G-CSF treatment locally increased cellularity after rotator cuff repair, but failed to improve structural healing [ 150 ]. By contrast, accelerated wound healing was found after topical G-CSF application [ 151 ].…”

Section: Neutrophils As a Therapeutic Target In Traumamentioning

confidence: 99%

Neutrophils in Tissue Trauma of the Skin, Bone, and Lung: Two Sides of the Same Coin

Kovtun

Messerer

Scharffetter‐Kochanek

et al. 2018

Journal of Immunology Research

111

101

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Following severe tissue injury, patients are exposed to various danger- and microbe-associated molecular patterns, which provoke a strong activation of the neutrophil defense system. Neutrophils trigger and modulate the initial posttraumatic inflammatory response and contribute critically to subsequent repair processes. However, severe trauma can affect central neutrophil functions, including circulation half-life, chemokinesis, phagocytosis, cytokine release, and respiratory burst. Alterations in neutrophil biology may contribute to trauma-associated complications, including immune suppression, sepsis, multiorgan dysfunction, and disturbed tissue regeneration. Furthermore, there is evidence that neutrophil actions depend on the quality of the initial stimulus, including trauma localization and severity, the micromilieu in the affected tissue, and the patient's overall inflammatory status. In the present review, we describe the effects of severe trauma on the neutrophil phenotype and dysfunction and the consequences for tissue repair. We particularly concentrate on the role of neutrophils in wound healing, lung injury, and bone fractures, because these are the most frequently affected tissues in severely injured patients.

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“…Mesenchymal stem cell transplantation [11][12][13][14] and administration of biological growth factors, such as recombinant human bone morphogenetic protein-2 (rhBMP-2) [6,14,15], transforming growth factor (TGF) -beta1 [16], fibroblast growth factor (FGF) [5] and granulocyte colony stimulating factor (G-CSF) [17], have been performed in recent studies. Though most studies using growth factors failed to form fibrocartilage tissue at the tendon-bone interface, Hashimoto et al reported that they succeeded in generating a tendon-bone junction similar to normal enthesis using rhBMP-2 in a rabbit model [15].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of tendon-bone interface healing and graft maturation with cylindrical titanium-web (TW) in a miniature swine anterior cruciate ligament reconstruction model: histological and collagen-based analysis

Ryu

Saito

Kurosaka

et al. 2020

BMC Musculoskelet Disord

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Background: Tendon-bone interface healing and ligamentization of the graft in anterior cruciate ligament (ACL) reconstruction with autografts are important factors affecting treatment outcome. This study aimed to investigate the effectiveness of a cylindrical titanium-web (TW) in tendon-bone interface healing and graft maturation in ACL reconstruction. Methods: Fourteen mature female CLAWN miniature swine underwent bilateral ACL reconstructions with patellar tendon (PT) autografts. In one limb, the TW/tendon complex was placed into the proximal side of the tibial tunnel. Only the graft was transplanted into the tunnel in the control limb. The proximal side of the graft was sutured into the stump of the native ACL and the distal end was stapled to the tibia. The animals were euthanized at 4 and 15 weeks postoperatively, for histological and biochemical analyses. Results: Microscopic images in TW limbs showed that ingrowth of tendon-like tissue and mineralized bone tissue into the TW connected the bone and the tendon directly. In contrast, fibrous tissue intervened between the bone and tendon in the control limbs. The total amount of collagen cross-links (which defines the strength of collagen fibers) and the maturation of collagen cross-links in TW tendons were significantly higher (p < 0.05) than those of control limbs. There was no significant difference in the ratio of dihydroxy-lysinonorleucine to hydroxylysinonorleucine (an indicator of tissue specific collagen maturation) between TW tendons and that of the native PT. Conclusions: TW promoted the maturation and formation of collagen cross-links in the grafted tendon while maintaining the cross-links pattern of native tendon collagen, and enabled direct binding of tendon to bone.

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The Effect of Granulocyte-colony Stimulating Factor on Rotator Cuff Healing After Injury and Repair

Abstract: Background The failure rate of tendon-bone healing after repair of rotator cuff tears remains high.

Cited by 26 publications

References 55 publications

Neupogen and mesenchymal stem cells are the novel therapeutic agents in regeneration of induced endometrial fibrosis in experimental rats

Neupogen and mesenchymal stem cells are the novel therapeutic agents in regeneration of induced endometrial fibrosis in experimental rats

Neutrophils in Tissue Trauma of the Skin, Bone, and Lung: Two Sides of the Same Coin

Enhancement of tendon-bone interface healing and graft maturation with cylindrical titanium-web (TW) in a miniature swine anterior cruciate ligament reconstruction model: histological and collagen-based analysis

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