In recent years, because of the decreasing liberation sizes of the minerals, processes such as grinding need to be evaluated in more detail. As is well known, size reduction processes are very important both in mineral processing and in many industrial applications. However, to increase the efficiency of the processes after size reduction, variations in particle morphology should also be evaluated, along with particle size. Although the effectiveness of grinding media (ball, rod, autogenous) has been shown for different materials, there are very few studies on the effect of the powder/grinding media ratio and grinding time on particle morphology in terms of shape factor and roughness values. This study aims to investigate the variations in the morphology of lignite samples under different grinding conditions such as grinding time and powder/grinding media ratio (U). The results of these analyses showed that while the d80 size of the ground lignite was 1.1 mm after 2 min grinding time, it decreased to 0.5 mm following 15 min grinding time. The roundness values of particles vary in the range of 0.746–0.790 with increasing grinding time. In addition to the grinding time, while the roundness of particles was found to be 0.739 for 0.34 U values (powder/grinding medium rate), it increased to 0.788 when the U value was adjusted to 0.67. The average roughness (Ra) values of particles increased from 60.9 nm to 107.9 nm upon increasing the grinding time from 2 min to 16 min. Due to these findings, it can be suggested that lignite samples became rounder with increasing grinding times, and roughness analyses made in a 10 × 10 μm surface area with an Atomic Force Microscope (AFM) indicated that particle roughness increased in direct proportion to grinding time.