The Effect of Growth Differentiation Factor 8 (Myostatin) on Bone Marrow–Derived Stem Cell–Coated Bioactive Sutures in a Rabbit Tendon Repair Model

Muraoka, Kunihide; Le, Wei; Behn, Anthony W.; Yao, Jeffrey

doi:10.1177/1558944718792708

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…Accordingly, the development of bioactive sutures for tendon repair has been investigated. These approaches include sutures coated with growth factors (e.g., PDGF, FGF, VEGF, GDF, and neurotrophin [199,[204][205][206]); mRNA [207]; ECM proteins such as HA [208]; stem cells [205]; and other bioactive agents such as aloe vera, curcumin, and chitosan [209] and antibacterial agents [210][211][212].…”

Section: Surgical Considerations For Tendon Injury In Elderly Patientsmentioning

confidence: 99%

Effect of Aging on Tendon Biology, Biomechanics and Implications for Treatment Approaches

Kwan,

Ng,

Rao

et al. 2023

IJMS

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Tendon aging is associated with an increasing prevalence of tendon injuries and/or chronic tendon diseases, such as tendinopathy, which affects approximately 25% of the adult population. Aged tendons are often characterized by a reduction in the number and functionality of tendon stem/progenitor cells (TSPCs), fragmented or disorganized collagen bundles, and an increased deposition of glycosaminoglycans (GAGs), leading to pain, inflammation, and impaired mobility. Although the exact pathology is unknown, overuse and microtrauma from aging are thought to be major causative factors. Due to the hypovascular and hypocellular nature of the tendon microenvironment, healing of aged tendons and related injuries is difficult using current pain/inflammation and surgical management techniques. Therefore, there is a need for novel therapies, specifically cellular therapy such as cell rejuvenation, due to the decreased regenerative capacity during aging. To augment the therapeutic strategies for treating tendon-aging-associated diseases and injuries, a comprehensive understanding of tendon aging pathology is needed. This review summarizes age-related tendon changes, including cell behaviors, extracellular matrix (ECM) composition, biomechanical properties and healing capacity. Additionally, the impact of conventional treatments (diet, exercise, and surgery) is discussed, and recent advanced strategies (cell rejuvenation) are highlighted to address aged tendon healing. This review underscores the molecular and cellular linkages between aged tendon biomechanical properties and the healing response, and provides an overview of current and novel strategies for treating aged tendons. Understanding the underlying rationale for future basic and translational studies of tendon aging is crucial to the development of advanced therapeutics for tendon regeneration.

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Section: Surgical Considerations For Tendon Injury In Elderly Patientsmentioning

confidence: 99%

Effect of Aging on Tendon Biology, Biomechanics and Implications for Treatment Approaches

Kwan,

Ng,

Rao

et al. 2023

IJMS

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“…Some modifications have been proposed: to improve cell adherence, Yao et al [ 80 ] added poly-L-lysine and fibronectin; Horváthy et al [ 81 ] covered previously sutures with albumin; and Casado et al [ 82 ] employed pre-treatments with gelatine and NaOH. Muraoka et al [ 83 ] added growth factors such as myostatin. Other authors have tested sutures solely impregnated by platelet-rich plasma[ 84 , 85 ] or VEGF[ 86 ] with interesting results.…”

Section: Discusionmentioning

confidence: 99%

Stem cell therapy applied for digestive anastomosis: Current state and future perspectives

Trébol¹,

Georgiev-Hristov²,

Migueláñez³

et al. 2022

WJSC

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BACKGROUND Digestive tract resections are usually followed by an anastomosis. Anastomotic leakage, normally due to failed healing, is the most feared complication in digestive surgery because it is associated with high morbidity and mortality. Despite technical and technological advances and focused research, its rates have remained almost unchanged the last decades. In the last two decades, stem cells (SCs) have been shown to enhance healing in animal and human studies; hence, SCs have emerged since 2008 as an alternative to improve anastomoses outcomes. AIM To summarise the published knowledge of SC utilisation as a preventative tool for hollow digestive viscera anastomotic or suture leaks. METHODS PubMed, Science Direct, Scopus and Cochrane searches were performed using the key words “anastomosis”, “colorectal/colonic anastomoses”, “anastomotic leak”, “stem cells”, “progenitor cells”, “cellular therapy” and “cell therapy” in order to identify relevant articles published in English and Spanish during the years of 2000 to 2021. Studies employing SCs, performing digestive anastomoses in hollow viscera or digestive perforation sutures and monitoring healing were finally included. Reference lists from the selected articles were reviewed to identify additional pertinent articles. Given the great variability in the study designs, anastomotic models, interventions (SCs, doses and vehicles) and outcome measures, performing a reliable meta-analysis was considered impossible, so we present the studies, their results and limitations. RESULTS Eighteen preclinical studies and three review papers were identified; no clinical studies have been published and there are no registered clinical trials. Experimental studies, mainly in rat and porcine models and occasionally in very adverse conditions such as ischaemia or colitis, have been demonstrated SCs as safe and have shown some encouraging morphological, functional and even clinical results. Mesenchymal SCs are mostly employed, and delivery routes are mainly local injections and cell sheets followed by biosutures (sutures coated by SCs) or purely topical. As potential weaknesses, animal models need to be improved to make them more comparable and equivalent to clinical practice, and the SC isolation processes need to be standardised. There is notable heterogeneity in the studies, making them difficult to compare. Further investigations are needed to establish the indications, the administration system, potential adjuvants, the final efficacy and to confirm safety and exclude definitively oncological concerns. CONCLUSION The future role of SC therapy to induce healing processes in digestive anastomoses/sutures still needs to be determined and seems to be currently far from clinical use.

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“…Using myostatin coated sutures to repair an injured rabbit Achilles tendon did not improve tendon mechanics 4 or 7 days following the repair (55). However, Muraoka and colleagues did find that the myostatin coated suture increased ColIa1 and ColIa2 mRNA more than 10-fold at both 4 and 7 days (55), suggesting that the early increase in collagen production has yet to be translated into mechanical properties.…”

Section: Myostatinmentioning

confidence: 99%

Muscle-tendon cross talk during muscle wasting

Avey

Baar

2021

American Journal of Physiology-Cell Physiology

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In organisms from flies to mammals, the initial formation of a functional tendon is completely dependent on chemical signals from muscle (myokines). However, how myokines affect the maturation, maintenance, and regeneration of tendons as a function of age is completely unstudied. Here we discuss the role of four myokines - fibroblast growth factors (FGF), myostatin, the secreted protein acidic and rich in cysteine (SPARC), and miR-29 - in tendon development and hypothesize a role for these factors in the progressive changes in tendon structure and function as a result of muscle wasting (disuse, aging and disease). Because of the close relationship between mechanical loading and muscle and tendon regulation, disentangling muscle-tendon crosstalk from simple mechanical loading is experimentally quite difficult. Therefore, we propose an experimental framework that hopefully will be useful in demonstrating muscle-tendon crosstalk in vivo. Though understudied, the promise of a better understanding of muscle-tendon crosstalk is the development of new interventions that will improve tendon development, regeneration, and function throughout the lifespan.

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The Effect of Growth Differentiation Factor 8 (Myostatin) on Bone Marrow–Derived Stem Cell–Coated Bioactive Sutures in a Rabbit Tendon Repair Model

Abstract: BMSC-coated bioactive sutures with GDF-8 do not appear to affect in vivo rabbit tendon healing within the first week following repair despite an increased presence of quantifiable RNA level of collagen. GDF-8's treatment efficacy of the early tendon repair remains to be defined.

Cited by 7 publications

References 35 publications

Effect of Aging on Tendon Biology, Biomechanics and Implications for Treatment Approaches

Effect of Aging on Tendon Biology, Biomechanics and Implications for Treatment Approaches

Stem cell therapy applied for digestive anastomosis: Current state and future perspectives

Muscle-tendon cross talk during muscle wasting

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