The Effect of Growth Factors on Biomechanical Properties of the Bone-Patellar Tendon-Bone Graft after Anterior Cruciate Ligament Reconstruction

Yasuda, Kazunori; Tomita, Fumihisa; Yamazaki, Shuji; Minami, Akio; Tohyama, Harukazu

doi:10.1177/0363546503261695

Cited by 86 publications

(57 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, it did not restore the elongated CrCL back to its normal length. In a bonepatellar tendon-bone graft CrCL reconstruction in dogs, intra-articular administration of TGF-b, together with epidermal growth factor, improved the structural properties of the autograft (Yasuda et al, 2004). These studies suggest that application of TGF-b has a positive effect on the collagen network in the injured CrCL and might have therapeutic use in an overstretched CrCL injury in dogs.…”

Section: Growth Factorsmentioning

confidence: 71%

Immunopathological mechanisms in dogs with rupture of the cranial cruciate ligament

Doom

Bruin

Rooster

et al. 2008

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

The majority of studies on cranial cruciate ligament (CrCL) disease to date have been carried out on dogs that already sustained a CrCL rupture, which is the end-stage of the disease. Investigations have recently been carried out to study humoral and cellular immunopathological mechanisms in predisposed dogs before clinical rupture of the contralateral CrCL. The cruciate ligaments are mainly composed of collagen type I, and immune responses to collagen have been suggested as a cause of CrCL degradation in dogs. None of these investigations showed evidence that anticollagen type I antibodies alone initiate CrCL damage. However, in predisposed dogs a distinct anticollagen type I antibody gradient was found towards the contralateral stifle joint that eventually sustained a CrCL rupture, suggesting that there was an inflammatory process present in these joints before detectable joint instability occurred. The importance of cellular reactivity to collagen type I in cruciate disease also remains unclear. Peripheral blood mononuclear cell proliferation to collagen type I was very diverse in dogs with cruciate disease whereas some sham operated dogs and healthy dogs tested positive as well. It is not yet determined whether cellular reactivity to collagen type I exists locally in the stifle joints nor whether this could initiate CrCL degradation.Inflammatory processes within the stifle joint can alter the composition of the cruciate ligaments. In animal models of immunemediated synovitis, the mechanical strength of the CrCL is significantly reduced. Immunohistochemical studies on synovial tissues from dogs with rheumatoid arthritis and dogs with cruciate disease revealed that the pathologic features are similar in both joint pathologies and that the differences are mainly quantitative. Joint inflammation induced by biochemical factors such as cytokines has been implied in CrCL degeneration. In several studies, the levels of pro-inflammatory and T helper cytokines were measured in dogs that sustained a CrCL rupture, but the exact role of the various cytokines in the pathogenesis of CrCL disease remains inconclusive. More recently, the levels of the cytokines have been investigated over time in predisposed dogs before and after CrCL rupture. IL-8 expression tended to be higher in stifle joints that will rupture their CrCL during the next 6 months than in those that will not, indicating an inflammatory process in these joints before clinical rupture.

show abstract

Section: Growth Factorsmentioning

confidence: 71%

Immunopathological mechanisms in dogs with rupture of the cranial cruciate ligament

Doom

Bruin

Rooster

et al. 2008

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

show abstract

“…The tunnel area was smaller and the stiffness of the bone-graft-bone complex was higher in the OPG group compared to those in the RANKL group . Transforming growth factor (TGF)-β1 (Yamazaki et al, 2005), TGF-β1 combined with Epidermal Growth Factor (EGF) (Yasuda et al, 2004) and Bone Morphogenetic Protein (BMP)-2 (Rodeo et al, 1999) have been used to promote tendon graft to bone tunnel healing. The effectiveness of alendronate in ACL graft healing could not be compared to these previous studies as different animal models, grafts, tunnel sizes, tunnel orientation, graft fixation methods and follow-up times were used.…”

Section: Ppy Lui Et Al Alendronate Enhanced Acl Graft-tunnel Healingmentioning

confidence: 99%

Alendronate reduced peri-tunnel bone loss and enhanced tendon graft to bone tunnel healing in anterior cruciate ligament reconstruction

Lui

Lee²,

Mok³

et al. 2013

eCM

View full text Add to dashboard Cite

Peri-tunnel bone loss after anterior cruciate ligament (ACL) reconstruction is commonly observed, both clinically and experimentally. We aimed to study the effect and mechanisms of different doses of alendronate in the reduction of peritunnel bone loss and promotion of graft-bone tunnel healing in ACL reconstruction. Eighty-four ACL-reconstructed rats were divided into 4 groups. Alendronate at different dosages, or saline, were injected subcutaneously weekly, for 2 or 6 weeks post-reconstruction, for vivaCT (computed tomography) imaging, biomechanical tests, histology and immunohistochemistry. Alendronate significantly increased bone mass and density of tissue inside bone tunnels except at the epiphyseal region of tibial tunnel. The femoral tunnel diameter decreased significantly in the mid-dose and highdose alendronate groups compared to that in the saline group at week 6. Alendronate significantly increased the peri-tunnel bone mass and density along all tunnel regions at week 6. Better graft-bone tunnel integration and intra-tunnel graft integrity were observed in the alendronate groups. The ultimate load was significantly higher in the mid-dose and high-dose alendronate groups at week 2, but not at week 6. There was a reduction in matrix metalloprotein (MMP)1, MMP13 and CD68-positive cells at the peri-tunnel region and graft-bone interface in the alendronate-treated group compared to the saline group. Alendronate reduced peritunnel bone resorption, increased mineralised tissue inside bone tunnel as well as histologically and biomechanically promoted graft-bone tunnel healing, probably by reducing the expression of MMP1, MMP13 and CD68-positive cells. Alendronate might be used for reducing peri-tunnel bone loss and promoting graft-bone tunnel healing at early stage post-ACL reconstruction.

show abstract

“…TGF-β2/PDGF treatment has no significant effect compared to TGF-β2 single treatment [32]. On the other hand, combined local application of TGF-β1 and EGF improves the structural property of tendon graft in an ACL reconstruction model [33].…”

Section: Discussionmentioning

confidence: 96%

Combined use of bFGF and GDF-5 enhances the healing of medial collateral ligament injury

Saiga

Furumatsu

Yoshida

et al. 2010

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Basic fibroblast growth factor (bFGF) and growth and differentiation factor (GDF)-5 stimulate the healing of medial collateral ligament (MCL) injury. However, the effect of isolated and combined use of bFGF/GDF-5 remains still unclear. We investigated cellular proliferation and migration responding to bFGF/GDF-5 using rabbit MCL fibroblasts. Rabbit MCL injury was treated by bFGF and/or GDF-5 with peptide hydrogels. Gene expression and deposition of collagens in healing tissues were evaluated. bFGF/GDF-5 treatment additively enhanced cell proliferation and migration. bFGF/GDF-5 hydrogels stimulated Col1a1 expression without increasing Col3a1 expression. Combined use of bFGF/GDF-5 stimulated type I collagen deposition and the reorganization of fiber alignment, and induced better morphology of fibroblasts in healing MCLs. Our study indicates that combined use of bFGF/GDF-5 might enhance MCL healing by increasing proliferation and migration of MCL fibroblasts, and by regulating collagen synthesis and connective fiber alignment.

show abstract

The Effect of Growth Factors on Biomechanical Properties of the Bone-Patellar Tendon-Bone Graft after Anterior Cruciate Ligament Reconstruction

Abstract: Application of growth factors is a possible strategy to prevent graft deterioration in anterior cruciate ligament reconstruction.

Cited by 86 publications

References 36 publications

Immunopathological mechanisms in dogs with rupture of the cranial cruciate ligament

Immunopathological mechanisms in dogs with rupture of the cranial cruciate ligament

Alendronate reduced peri-tunnel bone loss and enhanced tendon graft to bone tunnel healing in anterior cruciate ligament reconstruction

Combined use of bFGF and GDF-5 enhances the healing of medial collateral ligament injury

Contact Info

Product

Resources

About