The Effect of Growth Medium Composition on X.campestris Metabolism in Producing Xanthan Gum

Abstract

In the fermentation industry, the role of medium is very important in increasing the growth rate of microbes. Nutrient Agar (NA) is one of the common medium used for growth and increase regeneration X.campestris, while Luria-Bertani medium (LB) is usually used for matters related to X.campestris productivity in producing xanthan gum. To increase the productivity of X.campestris and xanthan gum rendement, this research used medium variation of Nutrient Broth (NB) added with mineral plus inducer and Luria-Bertani Broth (LBB) with 4% substrate of liquid sugar and 2% maize, respectively. Based on FT-IR analysis, both substrates can produce xanthan gum which qualitatively has been compatible with commercial xanthan gum. The 4% liquid sugar yielded of 34.20% - 57.88%, while 2% maize was 54.93% - 73.61%. The use of NB and LBB produced xanthan gum with viscosity of 28-31 cPs and 15-30 cPs, respectively. These viscosity values indicated that xanthan gum can be used as a thickener in its application in industry. Precipitation using a combination of salt with IPA did not provide significant changes to the xanthan gum gain. This is due to the low number of bonds between the cations of the salt and the anions of the xanthan solution.

Prospects of Biogenic Xanthan and Gellan in Removal of Heavy Metals from Contaminated Waters

“…The xanthan molecule is composed of pentasaccharide repeating units which consist of a cellulose-like backbone with a trisaccharide side-chain of two mannose and one glucuronate residue attached to a β-D-glucose unit, with pyruvic acid linked to O(4) and O(6) of the terminal D-mannose [ 77 ] and an acetyl group positioned at O(6) of the main chain D-mannose unit. The abundance of pyruvate and acetal groups of xanthan depends on the medium composition, the Xanthomonas strain, as well as the operational conditions used [ 78 ].…”