The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae

Abstract

Abbreviations used: DTNB -5,5'-dithiobis-(2-nitrobenzoic acid); H 2 R 123 -dihydrorhodamine 123; MES -2-morpholinoethanesulfonic acid; ROS -reactive oxygen species; SOD -superoxide dismutase; YPD medium -1% yeast extract, 1% Bacto-peptone, 2% glucose, YPG medium, 1% yeast extract, 1% Bacto-peptone,2% glycerol; YPE medium -1% yeast extract, 1% Bacto-peptone, 3% ethanol Abstract: We compared the oxidation of dihydrorhodamine 123, glutathione contents and activities of superoxide dismutase (SOD) and catalase for three wild-type strains of Saccharomyces cerevisiae grown on media with different carbon sources. The rate of oxidation of dihydrorhodamine 123 was much higher in respiring cells grown on ethanol or glycerol media than in fermenting cells grown on glucose medium. The total SOD activity was highest on glycerol medium and lowest on ethanol medium, while the catalase activity was highest on glycerol medium. The sequence of glutathione content values was: glucose > ethanol > glycerol.

Trx2p-dependent Regulation of Saccharomyces cerevisiae Oxidative Stress Response by the Skn7p Transcription Factor under Respiring Conditions

“…Our results reveal that wine yeasts are more resistant to several oxidant compounds under fermenting conditions than under respiratory conditions, which contrasts with previous data for lab yeasts [46,47]. These results can be explained by the ¨domestication¨ and adaptation of wine yeast to the fermentative metabolism of sugars over centuries, which has made them more resistant to different stresses under fermenting conditions [45,48].…”

Biophysical Characterization of Iron in Mitochondria Isolated from Respiring and Fermenting Yeast

“…The mitochondrial transcriptome changes more dramatically, with transcripts of cytochrome c isoform 1 and Mn-superoxide dismutase (MnSod2) declining under fermentation (51–52). Other groups have also reported lower SOD2 protein and transcript levels under fermentation (53–54). Our results are consistent, including the 3-fold increase in the Mn concentration of respiring mitochondria relative to respiro-fermenting and fermenting conditions which might reflect changes in the levels of MnSod2 or associated Mn species.…”

Plant Aquaporin AtPIP1;4 Links Apoplastic H2O2 Induction to Disease Immunity Pathways

“…Engineered yeast cells were cultured in liquid Yeast Extract-Peptone-Dextrose medium (Macierzy nska et al, 2007) for 16 to 18 h in a shaker at 160 rpm and 30°C, centrifuged at 1,000 rpm, and washed twice with phosphate buffer solution (PBS; 0.2 mM, pH 7.4). Precipitated cells were suspended with PBS containing an ROSprobing dye, incubated for 30 min, and then supplied with H 2 O 2 .…”

“…The yeast strain NMY51 was incubated in liquid Yeast Extract-PeptoneDextrose medium (Macierzy nska et al, 2007) containing 0 (control), 1, 2, or 3 mM H 2 O 2 in a shaker at 160 rpm and 30°C. One hour later, the yeast culture suspensions were centrifuged for 10 min at 700 rpm and 4°C.…”

“…Then, infiltrated leaf moieties were placed cut side down into a centrifuge tube, separated by nylon mesh from the glass bead padding on the bottom of the centrifuge tube, and centrifuged at 1,000 rpm to remove intracellular fluids (Sang et al, 2012). Centrifuged leaf samples were used in SOD extraction (Macierzy nska et al, 2007). All operations were carried out at -4°C, and the yeast or plant material was amended with phenylmethylsulfonyl fluoride at 100 mg mL 21 to inhibit enzyme decomposition.…”