The effect of growth regulators on the regeneration ability of flax ( Linum usitatissimum L.) hypocotyl explants in in vitro culture

Abstract

The aim of the present study is to determine the influence of different concentrations of growth regulators on the hypocotyl explants regeneration abilities of flax cultivars (Linum usitatissimum L.). Plant material used in the study was obtained from two flax cultivars: Modran and Selena. Hypocotyl explants were obtained from 6-dayold seedlings and were subsequently placed on basal MS (Murashige and Skoog 1962) medium and MS medium supplemented with 6-benzylaminopurine (BAP) or naftalene acetic acid (NAA). Final evaluation of the callus, the shoot and the root formation of the explants was performed 28 days after the experimental setup. The highest regeneration effectiveness was observed on the media supplemented with 1 mg/l BAP.

Screening of Five and Six-Membered Nitrogen-Containing Heterocyclic Compounds as New Effective Stimulants of Linum Usitatissimum L. Organogenesis in Vitro

“…It was shown that light green compact calli and shoot proliferation were obtained from hypocotyl explants on medium MS-BN containing 1 mg/l BAP and 0.05 mg/l NAA. Similar studies were conducted in the works [55,66,[74][75][76][77][78]. The inducing effect of various concentrations of growth regulators NAA, BAP, TDZ and 2iP added into basal MS medium on the formation of callus, shoot and root from hypocotyl explants of Linum usitatissimum L. was studied [55].…”

“…Data of our previous researches and numerous data of other authors witness that regeneration ability of flax (Linum usitatissimum L.) is under the influence of various types of growth regulators and their different concentrations in the culture media as well as origin of the plant explants [55,[62][63][64][65][66][75][76][77][78].…”

“…Linum usitatissimum L. (flax or linseed) is one of the oldest economically important crops that are widely used in biotechnology [54][55][56][57][58][59][60]. Linum usitatissimum L. belongs to Linaceae family which consists of more than 300 species mainly known as ornamental plants, but only one species Linum usitatissimum L. has a practical importance [55].…”

“…Linum usitatissimum L. belongs to Linaceae family which consists of more than 300 species mainly known as ornamental plants, but only one species Linum usitatissimum L. has a practical importance [55]. Flax fiber and seed have industrial significance as a source of cellulosic fiber for textile and paper industry and seed oil for pharmaceutical, cosmetic and food industry [56,57].…”

“…Because of using Linum usitatissimum L. in the biotechnology as an important source for bioactive compounds, numerous studies devoted to elaboration of effective techniques for in vitro regeneration of this plant have been conducted for many years [54,55,[61][62][63][64][65][66]. Nevertheless, the elaboration of new efficient methods for in vitro cultivation and regeneration of this plant is very actual problem today.…”