The eggshell, which is a complex and highly ordered structure, is very important
factor for food safety and egg marketing. This study investigated the changes in
eggshell structure and shell components in relationship to hen age. For this
study, we examined the histological change of the endometrium of the 30-, 60-,
and 72-wk-old commercial layers, and analyzed the ultrastructure and ionic
composition of their eggshells. The results showed that histological
deformation, fibrosis, atrophy and elimination of micro-villi in the uterus
endometrium were found through microscopic observation that was associated with
increasing hen age. Concentration of blood-ion components such as
Ca2+, Na+, K+, and
Cl– ions did not change with age. Along with the results
from the ultrastructure analysis of the eggshell, the palisade layer ratio and
the density of mammillary knobs were significantly decreased in older hens. In
addition, the type B mammillary knobs were frequently observed with increasing
hen age. In the mineral element assay from the eggshell, Ca2+,
S2–, and Co2+ significantly decreased
with increasing hen age, whereas Na+, K+,
and V2+ significantly increased. Therefore, the damages of
endometrial tissue inhibit the processes of ion transmission and the
crystallization of eggshell formation, resulting in a large and non-uniform
mammillary knob formation. This means the conditions of endometrial cells affect
the formation of the eggshell structure. In conclusion, hen aging causes the
weakness of the eggshell and degrades the eggshell quality.