The effect of heparin, caffeine and calcium ionophore a 23187 on in vitro induction of the acrosome reaction in frozen-thawed bovine and caprine spermatozoa

Pereira, Rosie; Tuli, R. K.; Wallenhorst, S.; Holtz, W.

doi:10.1016/s0093-691x(00)00340-x

Cited by 34 publications

(31 citation statements)

References 25 publications

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“…Several procedures have been designed to remove diluent media, cryoprotectants or decapacitating factors from the seminal plasma. Among these methods are swim-up (Berger et al, 1985;Parrish and Foote, 1987), the Percoll discontinuous gradient (Grant et al,1994;Jeong and Yang, 2001), Sephadex gel filtration (Steeno et al, 1985), glass wool filtration (Pereira et al, 2000), glass bead columns (Daya et al, 1987) and albumin gradients (Han et al, 1999;Iwasaki et al, 1999;Sun et al, 2001). However, some research teams prefer not to treat spermatozoa before IVF, but concentrate them by centrifugation before dilution in the fertilization medium (Martinez et al, 1996;Gadea and Matas, 2000).…”

Section: Introductionmentioning

confidence: 99%

Effect of sperm preparation method on in vitro fertilization in pigs

Matás¹,

Coy²,

Romar³

et al. 2003

Reproduction

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This study was designed to determine the effect of different sperm preparation treatments before IVF on the acrosome reaction, oocyte penetration time, early embryo development and timing of female and male pronucleus formation. Pooled sperm-rich fractions were (i) washed in PBS, (ii) left unwashed, or (iii) layered in a Percoll gradient. In Expt 1, the proportion of acrosome-reacted spermatozoa, determined by staining with fluorescein isothiocyanate-labelled peanut agglutinin lectin and propidium iodide, was highest after treatment with Percoll (P < 0.001). In Expt 2, oocytes matured in vitro were co-cultured with spermatozoa for 2, 4 or 6 h. Attached spermatozoa were then removed and the oocytes were cultured in fresh IVF medium for 16 h. Both sperm treatment and co-culture time were found to affect penetrability and monospermy rates (P < 0.001); spermatozoa treated with Percoll showed fastest oocyte penetration and highest penetrability. In Expt 3, matured oocytes were co-incubated with spermatozoa pretreated by the procedures (i), (ii) and (iii) for 2, 6 and 2 h respectively. Putative zygotes were then washed and transferred to medium NCSU-23 until the blastocyst stage. In this experiment, sperm treatment had a significant effect on the cleavage rate (P < 0.001) and rate of blastocyst formation (P < 0.05); the group treated with Percoll showed the highest rate of blastocyst formation. Finally, in Expt 4, timing of female and male pronucleus formation for each sperm treatment was determined 4, 6 and 8 h after insemination. The time of female and male pronucleus formation was affected by the sperm treatment and was faster for the Percoll group (P < 0.05). The findings of the present study indicate that treatment with Percoll yields the best results in this in vitro pig embryo production system.

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Section: Introductionmentioning

confidence: 99%

Effect of sperm preparation method on in vitro fertilization in pigs

Matás¹,

Coy²,

Romar³

et al. 2003

Reproduction

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“…In order to induce the acrosome reaction, culture media for in vitro oocyte fertilisation are supplemented with different agents, in cattle preferentially with heparin, which stimulates capacitation of bovine spermatozoa (Parrish et al 1988;Pereira et al 2000;Mendes et al 2003;Parrish 2014). Authors who have described the behaviour of spermatozoa during capacitation and acrosome reaction onset reported that the first changes in acrosome morphology appear after 2 h, independently of capacitation conditions (Bilaspuri and Babbar 2007).…”

Section: Discussionmentioning

confidence: 99%

“…In order to induce the acrosome reaction, culture media for in vitro fertilisation are supplemented with different capacitating agents, preferentially with heparin, which has a capacitating effect on bovine spermatozoa (Van Soom and de Kruif 1996;Pereira et al 2000;Mendes et al 2003). Sumantri et al (1996) demonstrated that under uniform capacitation conditions, the proportion of sperm capacitated at the same time is highly variable depending on the bull breed.…”

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confidence: 99%

Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa

Rečková¹,

Machatkova²,

Máchal³

et al. 2015

Vet. Med.

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This study was designed to investigate the characteristics of acrosomal changes during capacitation of bovine spermatozoa in relationship to in vitro fertility of individual bulls. Motile spermatozoa were separated from frozen-thawed semen by a swim-up procedure and capacitated in IVF-TALP medium with or without heparin. The spermatozoa were evaluated in terms of acrosomal changes at 0, 3, 4, 5, 6 and 8 h of capacitation. Proportions of acrosome-reacted spermatozoa at 5 h and 0 h of capacitation were used for calculation of the heparin response index. Variations in the heparin response index were found among individual bulls. Based on the mean response index value of all bulls, they fell into three categories: bulls with greater, intermediate and no response to heparin (GRH, IRH and NRH, respectively). Differences in the heparin response index between the bull categories were significant (P < 0.05). Higher D7 and D8 embryo development rates were found in the IRH vs. NRH bulls (P < 0.05). In conclusion, this study shows that the spermatozoa of bulls with a greater or intermediate response to heparin appear to be most suitable for in vitro embryo production compared with spermatozoa of bulls with no response to heparin.

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“…Tem sido sugerido que a adição de secreções do meio de cultivo de células de oviduto bovino às células espermáticas, em sistemas de produção in vitro de embriões, poderia contribuir para melhorias na fertilização in vitro e aumentar as taxas de clivagem e a produção de blastocistos [5].…”

Section: Introductionunclassified

Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados

et al. 2015

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Cómo citar este artículo: Valleriote P, Paes de Carvalho C, Dubeibe DF, Mogollón-Waltero ÉM, Burla-Dias, ÂJ. Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados. Spei Domus. 2015;11(22) Resumo. Introdução: nos bovinos, o oviduto é fundamental nos processos de maturação, fertilização e aquisição de competências para o adequado desenvolvimento do embrião até blastocisto. Visto que as células do revestimento interno do oviduto proporcionam substâncias que afetam positivamente os espermatozoides, à medida que atravessam o trato genital da fêmea, os componentes da superfície são modificados ou removidos pelas secreções ali presentes; ditas modificações podem incluir depleção do colesterol na superfície espermática, alteração nos glicosaminoglicanos e mudanças nos íons. Metodologia: Objetivou-se avaliar a adição de meio de cultura de células de oviduto bovino sobre parâmetros espermáticos como motilidade, vigor e integridade de membrana citoplasmática. Os espermatozoides foram incubados com 164 μl de Talp-sp-Uso no grupo controle (T Ct ); 161,5 μl de Talp-sp-Uso, acrescido de 2,5 μl de heparina (20µg/ml) no tratamento 1 (T Hep ) e 128 μl de Talp-sp uso, acrescido de 36 μl de meio de cultivo com secreções de células do oviduto bovino no Tratamento 2 (T Ov ). Foram avaliados no tempo zero (para mostrar que os meios dos tratamentos não prejudicaram os espermatozoides) e após três horas de incubação. Resultados: não foram encontradas diferenças significativas na motilidade progressiva, vigor e integridade de membrana celular entre tratamentos. Entretanto, espermatozoides cultivados com secreções de células do oviduto apresentaram maior percentagem de motilidade total às três horas de cultivo, em comparação a espermatozoides cultivados na presença de heparina. Conclusão: as secreções de células do oviduto não afetam a motilidade progressiva nem o vigor ou a integridade da membrana citoplasmática dos espermatozoides, porém prolongam a motilidade dessas células.Palavras-chave: capacitação espermática, células do oviduto, meio condicionado.

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The effect of heparin, caffeine and calcium ionophore a 23187 on in vitro induction of the acrosome reaction in frozen-thawed bovine and caprine spermatozoa

Cited by 34 publications

References 25 publications

Effect of sperm preparation method on in vitro fertilization in pigs

Effect of sperm preparation method on in vitro fertilization in pigs

Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa

Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados

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