The Effect of Heparin Concentration on Results of Venous Blood Gas of Patients admitted to Cardiac Intensive Care Unit: A double-blind clinical Trial

Hadipourzadeh, Fatemeh Shima; Lakziyan, Rasool; Shabani, Fatemeh; Sarchahi, Zohreh; Mazloomzadeh, Saeideh

doi:10.37796/2211-8039.1242

Cited by 1 publication

(1 citation statement)

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“…However, the natural ability of blood to coagulate in response to injuries or once removed from the body creates a challenge for researchers looking to effectively replicate blood in laboratory-based experiments. To overcome this challenge, many non-forensic researchers utilize blood that has been treated with an anti-coagulant such as EDTA, citrate, and/or heparin [4][5][6][7][8][9] to aid their experimental methodology. By adding the anti-coagulant, changes are made to the whole blood and thus the study subject, in order to prevent the premature clotting [10].…”

Section: Introductionmentioning

confidence: 99%

The effect of the anti‐coagulant EDTA on the deposition and adhesion of whole blood deposits on non‐porous substrates

Hughes,

van Oorschot,

Szkuta

et al. 2024

Journal of Forensic Sciences

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An investigation into whether the addition of a commonly used anti‐coagulant agent like ethylenediaminetetraacetic acid (EDTA) has an impact on the adhesion potential of blood to non‐porous substrates was conducted. Two non‐porous substrates (aluminum and polypropylene) exhibiting six different surface roughness categories (R1–R6) were used as test substrates upon which either whole blood or blood treated with EDTA was deposited. Samples were exposed to different drying periods (24 hours, 48 hours, and 1 week) before undergoing a tapping agitation experiment in order to evaluate the adhesion to the surface. Clear differences in adhesion potential were observed between whole blood and blood treated with EDTA. Blood treated with EDTA displayed a stronger adhesion strength to aluminum after a drying time of 24 h pre‐agitation, while whole blood presented with a stronger adhesion strength at the drying time of 48 h and 1 week. Both EDTA‐treated and EDTA‐untreated blood was shown to dislodge less easily on polypropylene with the only difference observed on smooth surfaces (0.51–1.50 μm surface roughness). Thus, when conducting transfer studies using smooth hydrophobic substrates like polypropylene or considering the likelihood of transfer given specific case scenarios, differences in adhesion strength of blood due to hydrophobic substrate characteristics and a decreased surface area need to be considered. Overall, whole blood displayed a better adhesion strength to aluminum, emphasizing that indirect transfer probability experiments using EDTA blood on substrates like aluminum should take an increased dislodgment tendency into account in their transfer estimations.

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