The effect of hepatocyte growth factor on turkey satellite cell proliferation and differentiation

Abstract

The effect of hepatocyte growth factor (HGF) on turkey satellite cell proliferation and differentiation was examined in cell culture. Satellite cell clones were established from one muscle of an individual turkey. The results showed that HGF is a potent activator and mitogen of turkey satellite cells and embryonic myoblasts with maximal stimulation at 1 ng/mL. HGF is also an inhibitor of differentiation of turkey satellite cells. Heterogeneity in the responsiveness to HGF in the turkey satellite cell population was observed between clones selected for fast (Early) or slow (Late) rates of proliferation. However, two other Early clones exhibited responses similar to those of two other Late clones. When combined with insulin-like growth factor (IGF) and fibroblast growth factor (FGF), singularly or in combination, HGF did not exert any additive or synergistic effects on Early or Late clone proliferation. Whereas when combined with IGF, FGF, and platelet-derived growth factor (PDGF), HGF significantly stimulated proliferation of the Late clone but not the Early clone. Addition of anti-HGF antibody to culture media diminished proliferation and provided evidence of autocrine production of HGF by turkey satellite cell cultures. Heterogeneity also exists in the turkey satellite cell population with respect to autocrine production of HGF.

The effect of temperature on apoptosis and adipogenesis on skeletal muscle satellite cells derived from different muscle types

“…Satellite cell heterogeneity exists between satellite cells taken from different fiber types (Feldman and Stockdale 1991;Lagord et al 1998;Huang et al 2006;Manzano et al 2011) in terms of variable rates of proliferation Collins et al 2005;Manzano et al 2011), differential expression of myogenic regulatory factors (Lagord et al 1998;Manzano et al 2011), and varying myogenic potentials (Lagord et al 1998;Yada et al 2006;Powell et al 2014a). Even satellite cells from the same fiber are heterogeneous and vary in proliferation rate (McFarland et al 1995;Rossi et al 2010), gene expression (Zammit et al 2004), and response to signaling molecules or growth factors (McFarland et al 1995(McFarland et al , 2003Yun et al 1997;Zeng et al 2002).…”

Dose-dependent modulation of myogenesis by HGF: implications for c-Met expression and downstream signalling pathways

“…MRFs are proteins expressed in cells committed to differentiate; together with Pax7, they are implicated in specification of the myogenic lineage (Buckingham et al, 2006;McFarlane et al, 2008;Seale et al, 2004). Differentiation studies published to date suggest an inhibitory effect of HGF (2.5-50 ng/ml) on myoblast differentiation (Gal-Levi et al, 1998;Leshem et al, 2000;Yamane et al, 2004;Zeng et al, 2002). Accumulating evidence implies that HGF may influence myogenesis via its regulation of MyoD, Myf-5, and myogenin (Charge & Rudnicki, 2004;Rosen et al, 1990).…”

Functional properties of muscle-derived cells related to morphological characteristics

“…Heterogeneity of SC in relation to their response to environmental stimuli has been demonstrated by identiWcation of sub-populations that displayed diVerential sensitivity to growth factor requirements for proliferation (Yablonka-Reuveni and Rivera 1994;McFarland et al 1995) or irradiation (Heslop et al 2000). It is interesting to observe that SC selected for fast and slow rates of proliferation have been shown to be heterogeneous for their responsiveness to the hepatocyte growth factor (HGF) (Zeng et al 2002). HGF is the Wrst growth factor reported to activate quiescent SC through interaction with the c-met receptor (Cornelison and Wold 1997) and cause them to enter the cell cycle (Allen et al 1995;Tatsumi et al 1998).…”