The Effect of High and Very Low Fluorescent Light Exposure Levels on Age-related Cataract in a Pigmented Mouse Strain

Wolf, Norman S.; Penn, Philip

doi:10.1006/exer.2001.1010

Cited by 7 publications

(6 citation statements)

References 32 publications

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“…Conversely, Sideris et al (1981) showed these lesions to be lethal to some Chinese hamster cells in culture. Wolf and Penn (2001) did not ®nd any effect from long-term¯uorescent light exposure on the incidence of cataract in mice, while Delcourt et al (2000) found a higher incidence of cortical and mixed cataract in human subjects who are exposed to excessive sunlight.…”

Section: Discussionmentioning

confidence: 70%

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White Light-mediated DNA Strand Breaks in Lens Epithelial Cells

Singh

Penn

Pendergrass

et al. 2002

Experimental Eye Research

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Section: Discussionmentioning

confidence: 70%

“…Lens epithelial cells provide a good model for relatively early age-related increase in oxidative stress (Wolf and Penn, 2001). Stress has been shown to be associated with DNA damage (Harman, 1998).…”

Section: Introductionmentioning

confidence: 99%

White Light-mediated DNA Strand Breaks in Lens Epithelial Cells

Singh

Penn

Pendergrass

et al. 2002

Experimental Eye Research

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“…To assess cataract formation, cataracts were read blindly using a hand-held slit lamp at a 30 degree angle after dilation with 1% tropicamide. Both eyes were scored on an opacity scale of 0, 1, 2, 3, or 4ϩ, with 4 representing complete lens opacity as described previously (47,48). The proliferative response of splenocytes was measured as described previously (24) in the presence of either T cell mitogens [concanavalin A (5 g/ml) or anti-CD3 antibody (2 g/ml)] or B cell mitogen lipopolysaccharide (LPS, 10 g/ml), anti-CD3 antibody (2 g/ml), or B cell mitogen LPS (10 g/ml).…”

Section: Methodsmentioning

confidence: 99%

Life-long reduction in MnSOD activity results in increased DNA damage and higher incidence of cancer but does not accelerate aging

Remmen

Ikeno

Hamilton

et al. 2003

Physiological Genomics

662

452

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Mice heterozygous for the Sod2 gene (Sod2+/- mice) have been used to study the phenotype of life-long reduced Mn-superoxide dismutase (MnSOD) activity. The Sod2+/- mice have reduced MnSOD activity (50%) in all tissues throughout life. The Sod2+/- mice have increased oxidative damage as demonstrated by significantly elevated levels of 8-oxo-2-deoxyguanosine (8oxodG) in nuclear DNA in all tissues of Sod2+/- mice studied. The levels of 8oxodG in nuclear DNA increased with age in all tissues of Sod2+/- and wild-type (WT) mice, and at 26 mo of age, the levels of 8oxodG in nuclear DNA were significantly higher (from 15% in heart to over 60% in liver) in the Sod2+/- mice compared with WT mice. The level of 8oxodG was also higher in mitochondrial DNA isolated from liver and brain in Sod2+/- mice compared with WT mice. The increased oxidative damage to DNA in the Sod2+/- mice is associated with a 100% increase in tumor incidence (the number of mice with tumors) in old Sod2+/- mice compared with the old WT mice. However, the life spans (mean and maximum survival) of the Sod2+/- and WT mice were identical. In addition, biomarkers of aging, such as cataract formation, immune response, and formation of glycoxidation products carboxymethyl lysine and pentosidine in skin collagen changed with age to the same extent in both WT and Sod2+/- mice. Thus life-long reduction of MnSOD activity leads to increased levels of oxidative damage to DNA and increased cancer incidence but does not appear to affect aging.

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“…Photographic examples of lens opacities graded 0 through 4ϩ are available in our previous publications. 4,7 A genome scan approach was used to detect marker loci with significant associations with lens opacity scores, by using an experiment-wise P Ͻ 0.05 as the principal significance criterion. This criterion, based on a permutation method, gives an indication of the likelihood that even one such gene-trait association could emerge by chance alone, given the observed data set, if the null hypothesis were correct, and thus adjusts the acceptance threshold to control type I errors for multiple comparisons of the many marker loci tested.…”

Section: Cataract Incidence In Relation To Age and Lineage Source Of mentioning

confidence: 99%

“…[1][2][3][4][5] In humans this condition results in visual limitations and, as indicated by the Framingham Eye Study, necessitates surgical intervention in 47% of persons in the United States between 65 and 74 years of age and in 73% of those older than 75 years. 6 The investigation of naturally occurring age-related cataract in pigmented mice (as opposed to light-induced cataracts in albino animals and cataracts produced in mice with mutations that influence early development of the eye) has been reported in only a few instances, although it is apparently a universal phenomenon, 4,7 probably because mice are rarely maintained into old age. The anatomic location of late life cataract in mice, mostly nuclear and posterior cortical, resembles that in otherwise healthy aging humans, 8 -12 and the incidence of lesions increases substantially in the last third of the lifespan in mice as in humans.…”

mentioning

confidence: 99%