The effect of high-protein and high-carbohydrate diets on [<sup>125</sup>I]iodoinsulin binding in skeletal muscle plasma membranes and isolated hepatocytes of rainbow trout (<i>Salmo gairdneri</i>)

Ablett, Richard F.; Taylor, Matthew; Selivonchick, Daniel P.

doi:10.1079/bjn19830080

Cited by 34 publications

(14 citation statements)

References 42 publications

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“…The number of binding sites on salmonid hepatocytes, and other binding indices are, however, similar to our previous observations on the salmons as well as to the data obtained on some other fish species (reviewed by Mommsen and Plisetskaya 1991) and on frog by Scapin and Incerpi (1992). In contrast, Ablett et al (1983) reported that parameters of insulin binding by trout hepatocytes are similar to that described in mammals.…”

Section: Discussionsupporting

confidence: 86%

“…Binding of insulin to isolated fish liver cells has been reported only by Ablett et al (1983), and Segner et al (1993) but to our knowledge, this is the first study which has examined insulin processing by fish liver cells. Mammalian insulin retains its full biological potency toward fish liver (reviewed by Mommsen and Plisetskaya 1991) and has been reported to be preferable over piscine insulins in 125I insulin binding studies (reviewed by Guti6rrez and Plisetskaya 1991a,b).…”

Section: Discussionmentioning

confidence: 86%

“…and Leibush and Bondareva (1987) were unable to demonstrate any effect of circulating hormone on insulin binding in different tissues of either wintering lampreys (Lampetra fluviatilis) or upstream migrating pink salmon (0. gorbuscha). In contrast, Ablett et al (1983) found indirect evidence of down-regulation of insulin receptors in the liver cells of trout fed on a high protein diet. Our previous results (Guti6rrez et al 1991;Guti6rrez and Plisetskaya 1991a,b) were also ambiguous.…”

Section: Introductionmentioning

confidence: 72%

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Insulin binding to isolated hepatocytes of Atlantic salmon and rainbow trout

Plisetskaya

Fabbri

Moon

et al. 1993

Fish Physiol Biochem

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The questions addressed in this study were: 1) whether insulin added to the incubation medium can down-regulate (125)I insulin binding to isolated hepatocytes of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss); 2) whether quantitative assessment of insulin processing can be made on isolated fish liver cells; 3) how ambient temperatures can affect insulin binding, and down-regulation of insulin receptors.After isolation and a short (up to 4h) "metabolic recovery period", liver cells were used either directly in (125)I insulin binding assay or first preincubated for 18h at 4°C or for 3h at 15°C, with or without mammalian or salmon insulin in concentrations ranging from 1 to 1000 nM.Preincubation at 15°C, decreased binding capacity (number of binding sites per liver cell) in all five independent hepatocyte preparations treated with 1000 nM insulin and in four out of five preparations treated with 100 nM insulin. At 4°C insulin binding sites were down-regulated in less than 50% of all hepatocyte preparations and only in the presence of 1000 nM insulin.Differential quantitive assessment was made of a) intact free insulin; b) insulin degraded; c) intact insulin bound to the cell membrane; d) internalized but degraded insulin, and e) intact insulin internalized by liver cells. Hepatocytes preincubated with 100 - 1000 nM insulin at 15°C bound and internalized less (125)I insulin.We hypothesize that in vivo, at water temperatures of 15°C and higher, extreme physiological levels of plasma insulin may regulate the numbers of insulin receptors in the salmonid liver. In contrast, in fish inhabiting cold waters the regulation of insulin receptors by circulating plasma insulin seems to be of little physiological importance.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 72%

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Insulin binding to isolated hepatocytes of Atlantic salmon and rainbow trout

Plisetskaya

Fabbri

Moon

et al. 1993

Fish Physiol Biochem

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“…Amino acids, rather than glucose, are the major stimuli for insulin release from the islet cells in teleosts (Ablett er ul. 1983) . Ablett et al (1983) suggested that high-protein diets chronically elevate insulin in salmon (Sulmo gairdneri) and reduce insulin binding by liver and muscle membranes.…”

Section: Discussionmentioning

confidence: 99%

Protein and energy relations in the broiler chicken

Rosebrough

Jp²,

Nc³

1989

Br J Nutr

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1. Broiler chickens growing from 7 to 28 d of age were given: (I) a 210 g protein/kg control diet for the entire experimental period, (2) an intermittent feeding regimen (210 g protein/kg diet for either 1 or 2 d followed by a 1 d fast), or (3) a daily change in the dietary protein level from 120 to 300 g/kg diet. Treatment variables examined were lipogenesis and glucose production in vitro, and circulating concentrations of insulin, triiodothyronine (T,) and thyroxine (TJ to determine the effects of chronic or acute dietary treatments.2. Giving the 300 g protein/kg diet or withholding feed for 1 d decreased (P < 005) lipogenesis in vitro compared with controls.3. Giving the 120 g protein/kg diet or refeeding with a 210 g protein/kg diet for 1 or 2 d increased (P < 0.05) lipogenesis in vitro compared with controls. Glucose production was affected in the same manner. 4.Fasting decreased (P < 0.05) plasma insulin and T, and increased T,. Both refeeding and a low-protein diet increased T,. Refeeding increased and a low-protein diet decreased insulin.5. Chronic use (7-28 d of age) of either an alternating protein or intermittent feeding regimen caused greater responses compared with acute bouts (single cycle) of either of the regimens.

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“…Muggeo et al (1979), when comparing insulin receptors from a variety of vertebrate species, described characteristics of insulin binding to erythrocytes of rainbow trout (Oncorhynchus mykiss). With respect to the liver of teleosts, Ablett et al (1983) were the first to study the specific interaction of insulin with hepatocytes. Kinetic data on insulin binding sites in teleostean liver including Scatchard plot analysis, saturation and displacement experiments, have been published to date only for the two closely related carnivorous species, coho salmon, (Oncorhynchus kisutch, and rainbow trout as well as for the scorpionfish (Scorpaena porcus, Leibush andBondareva 1991, cited by Gutierrez and.…”

Section: Introductionmentioning

confidence: 99%

Binding and bioactivity of insulin in primary cultures of carp (Cyprinus carpio) hepatocytes

Segner¹,

Böhm²,

Kloas³

1993

Fish Physiol Biochem

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Isolated carp hepatocytes cultured in serum-free, chemically defined medium were used to investigate within the same cell preparation characteristics of the binding of insulin as well as effects of insulin on cellular metabolism. The binding of human [(125)I]-insulin to carp hepatocytes was studied in kinetic, saturation and displacement experiments. A dependency of insulin binding on the collagenase used for cell isolation was demonstrated. Insulin binding decreased during the first 12h of culture but remained constant during the following 12h. The kinetic experiments revealed that [(125)I]-insulin binding reached a steady state within 20-30 min of incubation. The mathematical analysis of the saturation experiments demonstrated the existence of two populations of binding sites, one with high affinity (Kd1 = 5.5 pM) and low capacity (Bmax1 = 0.14 fmol/mg protein or 77 binding sites/cell) and one with low affinity (Kd2 = 2.4 nM) and high capacity (Bmax2 = 17.6 fmol/mg protein or 9623 binding sites/cell). In competition experiments, 312 pM [(125)I]-insulin was displaced by cold insulin, IGF-I and IGF-II with IC50 values of 2.2, 7.9 and 20.3 nM, respectively. Glucagon was without effect. Binding of insulin to carp hepatocytes resulted in a significant reduction of glucose release and a significant increase of protein synthesis as of de novo fatty acid synthesis.

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The effect of high-protein and high-carbohydrate diets on [¹²⁵I]iodoinsulin binding in skeletal muscle plasma membranes and isolated hepatocytes of rainbow trout (Salmo gairdneri)

Cited by 34 publications

References 42 publications

Insulin binding to isolated hepatocytes of Atlantic salmon and rainbow trout

Insulin binding to isolated hepatocytes of Atlantic salmon and rainbow trout

Protein and energy relations in the broiler chicken

Binding and bioactivity of insulin in primary cultures of carp (Cyprinus carpio) hepatocytes

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The effect of high-protein and high-carbohydrate diets on [125I]iodoinsulin binding in skeletal muscle plasma membranes and isolated hepatocytes of rainbow trout (Salmo gairdneri)

Cited by 34 publications

References 42 publications

Insulin binding to isolated hepatocytes of Atlantic salmon and rainbow trout

Insulin binding to isolated hepatocytes of Atlantic salmon and rainbow trout

Protein and energy relations in the broiler chicken

Binding and bioactivity of insulin in primary cultures of carp (Cyprinus carpio) hepatocytes

Contact Info

Product

Resources

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The effect of high-protein and high-carbohydrate diets on [¹²⁵I]iodoinsulin binding in skeletal muscle plasma membranes and isolated hepatocytes of rainbow trout (Salmo gairdneri)