The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells

Ibtisham, Fahar; Yun-feng, Zhao; Nawab, Aamir; Liguang, H; Wu, Jianli; Xiao, Mei; Zhao, Zhihui; An, Lilong

doi:10.1590/1806-9061-2017-0685

Cited by 15 publications

(7 citation statements)

References 37 publications

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“…Studies reported by Ibtisham et al (2018) noticed a mean value reduction of 16.4% in the chicken feed consumed by the animals housed in an unsuitable environment when compared to those housed in thermoneutral ambiance, in which air temperature was around 20 ºC. Therefore, Table 2 shows that, historically, Mato Grosso do Sul state, Brazil presents an unsuitable environment for poultry production.…”

Section: Resultsmentioning

confidence: 98%

Enthalpy thematic map interpolated with spline method for management of broiler chicken production

Silva

Santos

Zucca

et al. 2020

Rev. bras. eng. agríc. ambient.

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Owing to the exponential growth of the human population and problems related to food supply, research focused on finding the most suitable approach to manage and geographically explore the environment using sustainable technologies stand out. The present study aims to produce a consistent interpolation of historical series of enthalpy (H) resulting in a thematic map of enthalpy, using the spline method as a kriging option in areas with few sampling points. The thematic map considers thermal comfort conditions to produce broiler chickens, that could be used as a management tool to reduce power consumption due to the cooling process of the facilities. It was verified that spline is an efficient method to create a suitable thematic maps representations of areas presenting a few sampled units. The geographical representation of enthalpy allowed the evaluation of the environments, concluding that the state of Mato Grosso do Sul, Brazil is inadequate for broiler chickens production without suitable thermal cooling systems. Evidence suggests introduction of aviculture in areas still unexplored, e.g., Chapadão do Sul and Sete Quedas.

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Section: Resultsmentioning

confidence: 98%

Enthalpy thematic map interpolated with spline method for management of broiler chicken production

Silva

Santos

Zucca

et al. 2020

Rev. bras. eng. agríc. ambient.

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“…In spite of the higher physiological body temperature of birds, avian cells are often cultured at relatively lower temperatures, similarly to mammalian cultured cells. For instance, the aforementioned hepatic LMH cells, chicken embryonic fibroblast (CEF) cells, or chicken primary myocardial cells were cultured at 37 • C [15][16][17], while a chicken macrophage-like cell line was maintained at 41.5 • C [18]. Similarly, different protocols do exist for the in vitro modelling of heat stress.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, different protocols do exist for the in vitro modelling of heat stress. An elevation of the ambient temperature from 37 to 43 • C could trigger maximal heat stress response in LMH cells [15,23], while CEF cells were heat stressed by increasing temperature from 37 to 40-44 • C [16]. Further, heat stress response was evoked in myocardial cells by elevating temperature from 37 to 42 • C [17], and heat stress was modelled in chicken macrophage-like cells by incubating cells at 45 • C in contrast to control cells cultured at 41.5 • C [18].…”

Section: Discussionmentioning

confidence: 99%

“…After 24 h culturing, culture media were changed to fresh FBS-free Williams' Medium E, and confluent mono-and co-cultures on 6-well and 96-well plates were incubated at 43 • C for 1 or 2 h to mimic heat stress, while control cells were incubated further at 38.5 • C. The incubation conditions were set based on literature data and our pilot studies, considering that birds have higher physiological body temperature than mammals; however, cells isolated from avian species are often cultured at temperatures similar to mammalian cells. Normal incubation temperatures of avian cell cultures are ranging from 37 to 41.5 • C, and temperatures mimicking heat stress are varied between 40 and 45 • C [15][16][17][18], hence, as a compromise, 38.5 • C was chosen for maintaining control cells, and 43 • C for studying acute heat stress.…”

Section: Treatments and Measurements Of Cellular Metabolic Activity mentioning

confidence: 99%

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Effects of Acute Heat Stress on a Newly Established Chicken Hepatocyte—Nonparenchymal Cell Co-Culture Model

Mackei

Molnár

Nagy

et al. 2020

Animals

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Heat stress is one of the most important issues in broiler flocks impairing animal health and productivity. On a cellular level, excess heat exposure can trigger heat shock response acting for the restoration of cell homeostasis by several mechanisms, such as affecting heat shock protein synthesis, redox homeostasis and pro-inflammatory cytokine production. The major aim of this study was to establish a novel avian hepatocyte—nonparenchymal cell co-culture as a model for investigating the cellular effects of heat stress and its interaction with inflammation in chicken liver. Cell fractions were isolated by differential centrifugation from a freshly perfused chicken liver, and hepatocyte mono-cultures as well as hepatocyte–nonparenchymal cell co-cultures (with cell ratio 6:1, hepatocytes to nonparenchymal cells, mimicking a milder hepatic inflammation) were prepared. Isolated and cultured cells were characterized by flow cytometry and immunocytochemistry applying hepatocyte- and macrophage-specific antibodies. Confluent cell cultures were exposed to 43 °C temperature for 1 or 2 h, while controls were cultured at 38.5 °C. The metabolic activity, LDH enzyme activity, reactive oxygen species (H2O2) production, extracellular concentration of heat shock protein 70 (HSP70), and that of the pro-inflammatory cytokines interleukin (IL-)6 and IL-8 were assessed. Shorter heat stress applied for 1 h could strongly influence liver cell function by significantly increasing catabolic metabolism and extracellular H2O2 release, and by significantly decreasing HSP70, IL-6, and IL-8 production on both cell culture models. However, all these alterations were restored after 2 h heat exposure, indicating a fast recovery of liver cells. Hepatocyte mono-cultures and hepatocyte—nonparenchymal cell co-cultures responded to heat stress in a similar manner, but the higher metabolic rate of co-cultured cells may have contributed to a better capability of inflamed liver cells for accommodation to stress conditions. In conclusion, the established new primary cell culture models provide suitable tools for studying the hepatic inflammatory and stress response. The results of this study highlight the impact of short-term heat stress on the liver in chickens, underline the mediatory role of oxidative stress in acute stress response, and suggest a fast cellular adaptation potential in liver cells.

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“…Cell viability, apoptosis, proliferation, and oxidative status of cells in culture medium can be altered with high temperature. ROS (reactive oxygen species) formation increased with increasing Temperature (Proportionally) [17]. During the transportation must be care full about temperature.…”

Section: Effect Of Temperaturementioning

confidence: 99%

A Brief Concept of Cell Culture: Challenges, Prospects and Applications

Salauddin¹

2022

Cell Culture - Advanced Technology and Applications in Medical and Life Sciences

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Cell culture is an in vitro technique in which cells, tissues, or organs (animal origin) are artificially grown with the support of an artificial environment that encompasses culture medium, CO2 level, pH indicator, temperature keeping tissues alive and growing appropriately. Organ culture, Primary explant culture, and Cell culture among them cell culture widely used for the understanding of cell growth, normal functions, identification of growth factors, viral vaccine development, recombinant DNA (rDNA) technology, and immunobiological research. Due to high feasibility, cell culture practices highly demandable in the pharmaceutical industry. As well as animal cell culture used in laboratory research to study the cytotoxicity of new drug metabolic studies, aging, therapeutic proteins, the effects of drugs and toxic compounds on the cells and mutagenesis and carcinogenesis. There are a lot of issues in cell culture, Mycoplasma is one of the major. During cell culture, a single antibiotic often cannot kill the mycoplasma. Besides, culture media, pH indicator, incubation, cryopreservation, thawing, passaging of cells, and trypsinization have a great impact on cell culture. This chapter will help the reader to understand the whole process of cell culture and its applications, which will take them one step forward in their virology and cell culture research along with inspiration. This chapter also aids in the concept of cell count, cell suspension, CCF measurement, MOI (Multiplicity of Infection), and cell infection. Eventually, the reader will get a crystal clear concept of cell culture.

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The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells

Cited by 15 publications

References 37 publications

Enthalpy thematic map interpolated with spline method for management of broiler chicken production

Enthalpy thematic map interpolated with spline method for management of broiler chicken production

Effects of Acute Heat Stress on a Newly Established Chicken Hepatocyte—Nonparenchymal Cell Co-Culture Model

A Brief Concept of Cell Culture: Challenges, Prospects and Applications

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