The effect of homologous prostatic fluid on motility and morphology of dog epididymal spermatozoa extended and frozen in Biladyl with Equex STM paste or Andromed

Abstract

Although dog prostatic fluid decreases the longevity of ejaculated dog spermatozoa, it also increases their rate of motility and their fertility after vaginal insemination, as well as the fertility of epididymal spermatozoa after uterine insemination. These findings indicate a need to further characterize the effects of prostatic fluid on dog spermatozoa. This study was done to determine the effects (P < 0.05) of homologous prostatic fluid added prior to cooling, after thawing, or at both times to epididymal spermatozoa from 21 dogs. The

The Quality of Cryopreserved Sperm Collected from Feline Caudal Epididymides Using Seminal Plasma

“…For example, in Iberian red deer [6], no difference due to the presence or absence of SP was noted in epididymal sperm motility before freezing, but motility was significantly higher when sperm were treated with SP after freezing-thawing. The same phenomenon has been reported [4,7] in dogs. In horses [3], it has been reported that the conception rate in artificial insemination was increased by addition of SP with either non-frozen or frozen-thawed sperm.…”

“…These may have influenced sperm and reduced their motility. In other animal species, improvement in sperm qualities or fertility by SP has been frequently reported [3,4,6,7]. For example, in Iberian red deer [6], no difference due to the presence or absence of SP was noted in epididymal sperm motility before freezing, but motility was significantly higher when sperm were treated with SP after freezing-thawing.…”

“…However, no difference was noted in lectin binding between feline epididymal and ejaculated sperm. The effects of SP on sperm functions have been reported to be positive or negative in various animal species [3,4,[6][7][8]11].Kawakami et al[5] investigated protein attachment on the surfaces of canine caudal epididymal and ejaculated sperm. They observed that PHA-E lectin did not bind to caudal epididymal sperm but bound to ejaculated sperm and suggested that a PHA-E lectin-binding glycoprotein, N-acetyl-D-galactosamine, is a decapacitation factor.…”

“…However, no difference was noted in lectin binding between feline epididymal and ejaculated sperm. The effects of SP on sperm functions have been reported to be positive or negative in various animal species [3,4,[6][7][8]11].…”

Artificial Insemination in Veterinary Science

“…Epididymal spermatozoa have been harvested from a variety of species like cats (Filliers et al, 2008;Hermansson & AxnÈr, 2007), dogs (Garcia-Macias et al, 2006;Hewitt et al, 2001;Nothling et al, 2007;Ponglowhapan et al, 2006), rats (Yamashiro et al, 2007), horses (Braun et al, 1994;Bruemmer, 2006;Heise et al, 2011;Johnson & Coutinho da Silva, 2008;Melo et al, 2008), cattle (Goovaerts et al, 2006;Martins, Rumpf, Pereira, & Dode, 2007), pigs (Ikeda et al, 2002), sheep (Garcia-Macias et al, 2006), goats (Blash, Melican, & Gavin, 2000), red deer (Fernandez-Santos et al, 2006;Garcia-Macias et al, 2006;MartÌnez-Pastor et al, 2006), Spanish Ibex , African buffalo (Herold et al, 2006), North American buffalo (Lessard at al., 2009) and monkeys (Goff et al, 2009;Ng et al, 2002). Pregnancies and offspring after AI with epididymal spermatozoa have been produced amongst others in horses (Barker & Gandier, 1957;Heise et al, 2010;Papa et al, 2008), dogs (Hori, Hagiuda, Kawakami, & Tsutsui, 2005) and Spanish Ibex (SantiagoMoreno et al, 2006).…”