The effect of hormonal estrus induction on maternal effect and apoptosis-related genes expression in porcine cumulus-oocyte complexes

Bogacki, Marek; Wasielak, Marta; Kitewska, Anna; Bogacka, Iwona; Jalali, Beenu Moza

doi:10.1186/1477-7827-12-32

Cited by 13 publications

(8 citation statements)

References 42 publications

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“…P 4 regulates meiosis and ovulation by activating the progesterone receptors (60). A high level of P 4 in the FF increases the percentage of oocytes at the germinal vesicle stage, and the maternal P 4 level affects the gene transcripts of cumulus-oocyte complexes (54). By contrast, upon the lack of the necessary hormonal stimulation from P 4 , the development and maturation of oocytes would be delayed or may even not reach an acceptable level (61,62).…”

Section: Discussionmentioning

confidence: 98%

“…Although no correlation has been detected between the serum level of P 4 and the prognosis of assisted reproductive technology (ART) (52), the P 4 level in the FF only secreted and synthesized by granulosa cells is more closely correlated with the development of oocytes and the outcome of IVF (53,54). In the present study, the EM group with a higher FF AOPP concentration presented a lower FF P 4 level, thus a negative correlation was observed between AOPPs and P 4 levels.…”

Section: Discussionmentioning

confidence: 99%

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Advanced oxidation protein products from the follicular microenvironment and their role in infertile women with endometriosis

et al. 2017

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Abstract. Endometriosis (EM) is associated with oxidative stress. Advanced oxidation protein products (AOPPs) are novel markers of oxidative stress, which serve an important role as an inflammatory mediator in various chronic diseases. In order to examine the role of AOPPs in infertile women with EM, the present study analyzed the levels of AOPPs, estradiol (E 2 ) and progesterone (P 4 ) in the follicular fluid (FF) of 89 women with or without EM undergoing in vitro fertilization (IVF). The AOPP concentration in the FF of the EM group was significantly higher when compared with that of the control group (51.5±22.4 vs. 41.8±18.3 µmol/l; P<0.05). However, the FF P 4 levels and blastocyst rate were significantly lower in the EM group compared with the control group (P 4 :1,249.6±465.4 vs. 1,752.7±565.4 ng/ml, P<0.05; blastocyst rate: 0.511±0.322 vs. 0.662±0.278; P<0.05). The AOPP concentration and P 4 level in the FF presented a significant negative correlation in the EM and control groups, as well as in the total cohort of patients (EM group: r=-0.406, P=0.006; control group: r=-0.315, P=0.035; total: r=-0.421, P<0.001). In addition, there was a significant negative correlation between the FF AOPP concentrations and blastocyst rate in the EM group and in the total cohort (EM group: r=-0.376, P=0.012; total: r=-0.367, P<0.001). In conclusion, these results suggested that AOPPs may be a potentially effective marker for predicting the oocyte quality and outcomes of IVF in infertile women with EM.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Advanced oxidation protein products from the follicular microenvironment and their role in infertile women with endometriosis

et al. 2017

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“…The production of adequate amounts of BMP15 protein by the oocyte is necessary to promote cumulus cells expansion. The expression of BMP15 mRNA was also found in cumulus cells and it decreased along with the oocyte maturation and cumulus expansion in vitro (Bogacki et al 2014).…”

Section: Figurementioning

confidence: 94%

The expression of growth factor signaling genes in co-culture IVM

Setiawan

Kim

et al. 2018

Indones. J. Biotechnol.

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The objec ve of this study was to determine the expression of growth factor signaling genes in human adiposederived stem cells (ASCs), porcine oocytes, and cumulus during in vitro matura on (IVM). The human ASCs (from 2 young and 2 old donors) were used for the co-culture IVM system. The matura on rate was examined based on polar body extrusion. The expression of the growth factor signaling genes from ASCs, oocytes, and cumulus were measured using qPCR. All data were analyzed using ANOVA followed by Tukey's test. The expression of the h-IGF1 signaling genes from human ASCs cells showed similar values in all groups and the h-FGF2 expressions were higher in the young donors than the old ones. The p-FGF2, p-FGFR2, and p-TGFβ1 expressions in the oocytes as well as p-IGFR in the cumulus that were co-cultured from the young donors showed higher values than the old and control groups. The apopto c ra o (p-BAX/p-BCL2) from the oocytes and cumulus in both co-culture groups also showed lower levels than the control (P<0.05). Oocyte matura on rates were significantly increased in all co-cultured groups (Y1 (85.9 ± 2.2%), Y2 (91.2 ± 1.1%), O1 (86.3 ± 1.5%), and O2 (86.5 ± 2.3%)) compared with the control (76.7 ± 1.1%; P<0.05). Although the expression of growth factor signaling genes was varied, young donors' ASCs might support in vitro matura on be er than those from old donors.KEYWORDS co-culture; gene expression; human ASCs; oocytes matura on Introduc onIn vitro maturation (IVM) technique have been essential and important to retrieve high quality mature oocytes for optimum result in the further study, such as embryo production, xenotransplantation and animal model research (Woods and Tilly 2012;Coticchio et al. 2015;Arat et al. 2016). The oocyte is a unique and highly specialized cell responsible for creating, activating and controlling the embryonic genome, as well as supporting basic processes, such as cellular homeostasis, metabolism and cell cycle progression in the early embryo. Oocytes maturation were occurred when the oocytes reached nuclear and cytoplasmic maturation (Coticchio et al. 2015). Nuclear maturation encompasses numerous sequential events, including the breakdown of the germinal vesicle and the resumption of meiosis, the first meiotic division, and the appearance of second metaphase chromosomes. These processes can be evaluated cytologically by the appearance of the first polar body or metaphase II chromosomes (Machtinger et al. 2016). Cytoplasmic maturation occurs alongside of nuclear maturation, involving dramatic changes in oocyte gene expression, protein synthesis, and organelle organization (Woods and Tilly 2012;Machtinger et al. 2016).Cumulus cells have been considered to play an important role in oocyte maturation by keeping the oocyte under meiotic arrest, inducing meiotic resumption and by supporting cytoplasmic maturation. These functions have been attributed to their gap junctions and their specific metabolizing capabilities. Physical contact between oocyte and cumulus cells has been ...

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“…A large number of mRNAs and proteins named maternal effect genes aggregate during oocyte growth and cytoplasmic maturation. The processes of synthesis, storage, and function of these maternal transcripts strongly determine the quality and development potential of oocytes and embryos (15). Maternal effect genes that organize the transportation of methyl groups in the genome and chromatin modification of two pronuclei are essential for genome reprogramming processes after fertilization (16).…”

Section: Mortalities and Morbiditiesmentioning

confidence: 99%

Superovulation Affects the Gene Expression Patterns of Mice Oocytes and Preimplantation Embryos Produced by Different Assisted Reproductive Technologies

Jahanbakhsh-Asl¹,

Salehi²,

Ghaffari-Novin³

et al. 2018

International Journal of Women’s Health and Reproduction Scienc

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implantation loss (2-4). Recent studies provide a growing body of evidence on significant association between ovarian stimulation and increased incidence of certain imprinting diseases including Beckwith-Wiedemann and Angelman syndromes in humans as well as imprinting defects in the mice (5,6). Methylation marks established on imprinted genes in the mouse are stably preserved during the second wave of genome-wide demethylation and remethylation occurs in the late blastocyst close to the time of implantation to maintain sex-specific monoallelic expression of gene imprints (7,8). The establishment, maintenance, or acquisition of methylation marks during epigenetic reprogramming are thought to be vulnerable to some degree of error and may be disrupted due to various factors including exposure to certain environmental factors such as high doses of exogenous gonadotrophins (4,6). The hypothesis of the epigenetic effects of superovulation are supported by several detailed reports showing aberrant DNA methylation of imprinted genes Abstract Objectives: In this experiment, possible effect of superovulation on important molecular profiles of oocytes and embryos were examined in mice regarding their development. Materials and Methods: A total of 120 metaphase II oocytes were produced by super or spontaneous ovulation. Blastocyst-stage embryos were produced after in vitro fertilization (IVF) and in vivo fertilization. Blastocysts that have been obtained from naturally mated female without gonadotrophin treatment were also used as controls. Using real-time polymerase chain reaction (PCR), the mRNA expression patterns of Bone morphogenesis protein (Bmp 15(, hepatoma-derived growth factor (Hdgf(, DNA methyl transpherase-1 (Dnmt-1), developmental pluripotency associated 3 (Dppa3(, and zinc finger protein 57 (Zfp57(genes were compared between superovulated and spontaneously ovulated oocytes; the expression patterns of H19 and small nuclear ribonucleoprotein N(Snrpn) imprinting genes between embryos produced from these oocytes were also compared. Results: The results of the study showed that expression of five maternal effect genes (Bmp15, Hdgf, Dnmt-1, Dppa3, and Zfp57) in superovulated oocytes were significantly reduced (P<0.05). However, the rates of cleavage to the blastocyst stages were not significantly different (P<0.05) between superovulated and naturally ovulated oocytes in cultured embryos. Moreover, superovulation disturbed the expression patterns of imprinting genes (H19 and Snrpn) in single blastocysts obtained by different assisted reproductive techniques (ARTs). Conclusions: The findings indicated that superovulation affected the molecular characteristics of ART conceived embryos.

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The effect of hormonal estrus induction on maternal effect and apoptosis-related genes expression in porcine cumulus-oocyte complexes

Cited by 13 publications

References 42 publications

Advanced oxidation protein products from the follicular microenvironment and their role in infertile women with endometriosis

Advanced oxidation protein products from the follicular microenvironment and their role in infertile women with endometriosis

The expression of growth factor signaling genes in co-culture IVM

Superovulation Affects the Gene Expression Patterns of Mice Oocytes and Preimplantation Embryos Produced by Different Assisted Reproductive Technologies

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