The effect of human umbilical cord blood–derived mesenchymal stem cell media containing serum on recovery after laser treatment: A double‐blinded, randomized, split‐face controlled study

Abstract

Background Ablative CO2 fractional laser (AFL) is a common cosmetic procedure to improve skin laxity. However, due to prolonged downtime and the risk of postinflammatory hyperpigmentation, laser‐assisted delivery of active ingredients as post–laser treatment has gained interest in past years. Among various active ingredients, human umbilical cord blood–derived mesenchymal stem cells (hUCBMSCs) can be a promising agent promoting skin regeneration. Aims We evaluated the efficacy and safety of a human cord blood cell–conditioned media containing serum and cream on patients who underwent AFL treatment. A randomized, investigator‐blinded, prospective, split‐face comparison study was conducted. Materials and Methods Twenty‐three patients who underwent AFL on both cheeks applied a human umbilical cord blood–derived mesenchymal stem cell (hUCBMSC)‐conditioned media containing cream with or without stem cell containing serum application. As a primary outcome measure, we evaluated the total area of microcrusts and post‐treatment erythema using digital photographs. Additionally, skin biophysical parameters (corneometer, TEWL) and global improvement scores for skin texture were assessed. Results The area of total microcrusts was reduced in the study group which applied both serum and cream. The global improvement score of the post‐treatment erythema was significantly reduced. Investigator‐assessed global improvement scores were higher in the combination treatment group. Additionally, there was no adverse event, which was associated with the use of either hUCBMSCs containing serum or cream. Conclusion The application of human cord blood cell containing serum and cream resulted in accelerated wound healing and reduced post‐treatment erythema, which effectively reduced recovery time after ablative laser treatment.

The application of mesenchymal stromal cells (MSCs) and their derivative exosome in skin wound healing: a comprehensive review

“…Recently, a study on 24 patients with aging skin who underwent ablative fractional laser (AFL) treatment revealed that AT-MSCS-CM induced beneficial effects most likely by decreased MMP-1 and MMP-2 expression along with improved collagen 1 expression [ 165 ]. Likewise, administration of allogeneic UC-MSCs containing serum and cream instigated accelerated wound healing and diminished post-treatment erythema, and thereby exhibited the significant competence to support recovery after AFL [ 166 ]. Evaluation of the possible therapeutic merits of UC-MSCs transplantation in 53 patients (72 limbs) with DFU also exhibited that the intervention group experienced considerably greater and more stable amelioration in ankle-brachial pressure index, transcutaneous oxygen tension, skin temperature, and claudication distance compared to control group [ 167 ].…”

Human Umbilical Cord-Based Therapeutics: Stem Cells and Blood Derivatives for Female Reproductive Medicine

“…Notably, recent studies proposed using the factors secreted by hUC-SCs, rather than cell transplantation, for regenerative medicine [100][101][102][103]. The secreted GFs are referred to as the secretome of the hUC-SCs, can be isolated from culture media, and induce biological effects similar to SC therapy.…”

Mesenchymal Stem/Stromal Cells Derived from Human and Animal Perinatal Tissues—Origins, Characteristics, Signaling Pathways, and Clinical Trials

“…Kim et al reported that the topical drugs with umbilical cord-derived MSC conditioned media improved the skin stratum corneum and strengthened the skin barrier in patients with atopic dermatitis [ 316 ]. Finally, it was found that the application of umbilical cord blood-derived MSC conditioned media containing cream or serum reduced the total area of microcrusts and erythema in patients treated with ablative CO 2 fractional laser [ 317 ].…”