The Effect of Hydroalcoholic Extract of Junipers communis on Proliferation BHK Cells

Abstract

Nowadays, use of medicines from plant source increases significantly with conventional therapies. Juniper communis has been observed in a wide range of traditional medicinal systems as anticancer activities an appetite suppressant, diuretic, anti-inflammatory and analgesic agents, as well as for the treatment of wounds, renal disorders gastric used. This study examines the impact of juniper extracts on the viability of BHK cell line, which is considered as normal cells. BHK cell lines were divided into 6 groups including control, and groups receiving juniperus communis extract in 10mg/ml, 1mg/ml, 0.1mg/ml, 0.01mg/ml and 0.001mg/ml doses. After 48 hours the viability of cell lines was examined with MTT assay. The data was analyzed using ANOVA. Our findings show that , in Low doses of the extract on cell line BHK, had no effect and the increase in concentration, inhibit the proliferation of cells is) on 1 mg/ml and higher doses is toxic effect). The results show that there is a need to establish a safe dose for use of these plant extracts.