The Effect of Hydrogel Hyaluronic Acid on Dentine Sialophosphoprotein Expression of Human Dental Pulp Stem Cells

Abstract

Objective Hyaluronic acid (HA) is glycosaminoglycan and one of important factors in extracellular matrix. In an inflamed pulp, when niche biology is conducive, the recruitment of human dental pulp stem cells (hDPSCs) will take place and differentiate into odontoblast like cell, creating reparative dentine and expressing dentine sialophosphoprotein (DSPP). Therefore, the purpose of this study was to analyze the potential of hydrogel HA in various concentration towards hDPSCs differentiation via DSPP expression at day 7 and 14. Methods After hDPSCs incubation reaching 80% confluence, cells were then starved for 24 hours. Then, culture media were supplemented with osteogenic media. hDPSCs planted into 96 well plate and HA 10 µg/mL, 20 µg/mL, and 30 µg/mL were added. DSPP expression was analysed using elisa reader at day 7 and 14, qualitative result was analysed using alizarin red at day 21. Data was analysed using one-way ANOVA. Results At day 7, there was a statistically significant different potential of HA conditioned media in various concentration (p<0.05) towards hDPSCs differentiation via expression of DSPP with HA 30 µg/mL being the most potential concentration to increase DSPP expression. Conclusion HA have the potential to increase odontoblast differentiation process via expression of DSPP, with HA 30 µg/mL being the optimum concentration for hDPSCs.

Immunohistochemical study of mixing mineral trioxide aggregate with hyaluronic acid as a pulp-capping agent in dog teeth

“…This is in agreement with Umemura et al [ 19 ] who reported that culturing human dental pulp stem cells (DPSCs) in HA reported an elevation in mineralization and ALP activity after 7 days. The results of our study are also consistent with the results obtained by Chen et al [ 20 ] who stated that the application of HMW-HA demonstrated an elevation in mineralization rate on day 7 using the ALP activity test, and Nugraheni et al [ 21 ] who stated that after 7 days of observation, HA application increased and stimulated odontoblast differentiation.…”

“…Which can be related to a higher odontoblast differentiation rate. Nugraheni et al [ 21 ] stated that HA stimulates odontoblast differentiation up to 21 days of observation.…”