The effect of hydroxyurea and mitomycin C on sperm motility in mice

Hydroxycarbamide: a user’s guide for chronic myeloproliferative disorders

“…In mouse models, hydroxycarbamide produced testicular atrophy, suppressed spermatogenesis, reduced sperm motility, and induced abnormalities in sperm morphology and chromatin structure [84,85]. Experimental evidence suggested that this toxicity was reversible upon treatment withdrawal [86].…”

Influence of sickle cell disease and treatment with hydroxyurea on sperm parameters and fertility of human males

“…Patients with sickle cell disease often have moderate to severe hypogonadism, [9][10][11] of unknown origin, although several mechanisms have been suggested: primary hypogonadism, 9,12 hypogonadism induced by repeated testicular infarction, 13 zinc deficiency, 14,15 and puberty delay due to span-height retardation. [16][17][18] Hydroxyurea itself has been reported to impair spermatogenesis in mammals, resulting in testicular atrophy, 19-21 a reversible decrease in sperm count, [19][20][21][22][23] and abnormal sperm morphology 20,24 and motility. 19 Furthermore the chromatin structure of germ cells is also affected, mainly in preleptotene spermatocytes 20,21 and apoptosis is increased, essentially in spermatogonia and early spermatocytes 25 while stem spermatogonia do not seem to be affected, resulting in the possibility of repopulation of seminiferous tubules.…”

“…[16][17][18] Hydroxyurea itself has been reported to impair spermatogenesis in mammals, resulting in testicular atrophy, 19-21 a reversible decrease in sperm count, [19][20][21][22][23] and abnormal sperm morphology 20,24 and motility. 19 Furthermore the chromatin structure of germ cells is also affected, mainly in preleptotene spermatocytes 20,21 and apoptosis is increased, essentially in spermatogonia and early spermatocytes 25 while stem spermatogonia do not seem to be affected, resulting in the possibility of repopulation of seminiferous tubules.…”

Use of Sperm Enzymes to Detect Genotoxic Agents.

“…In a previous experiment (Ficsor and Ginsberg, 1980), a significant reduction in sperm motility was noted 5 weeks after 5 consecutive days of treatment with HU. This effect was not noted in the current study where the single injection regime was used.…”

“…In Exp 2, approximately 200 sperm per animal were used to determine motility using a Neubauer hemocytometer (Ficsor and Ginsberg, 1980). Acrosin Assay: Acrosin activity was determined according to methods described by Ficsor et al (1983).…”