The Effect of <i>Buddleja officinalis</i> Maxim Eye Drops on Morphology and Apoptosis in Lacrimal Gland of Experimental Dry Eye Rabbit Model

Zhou, Ya-Sha; Peng, Jun; Zhang, Youwei; Peng, Xiao-Fang; Peng, Qinghua; Yang, Yanzhu

doi:10.1155/2019/5916243

Cited by 8 publications

(5 citation statements)

References 10 publications

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“…Based on the etiology and classification of dry eye, different animal models have been developed over the years [4,5]. In rabbits, dry eye has been induced by many procedures such as dacryoadenectomy [6], female and male castration [7,8], desiccating stress [9], induced autoimmune dacryoadenitis [10], injection of the lacrimal gland with concanavalin A [11], injury of the drainage duct [12], nerve denervation [13], and topical instillation of atropine [14] or benzalkonium chloride (BAC) [15]. e current publication is focused on the topical instillation of BAC.…”

Section: Introductionmentioning

confidence: 99%

Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride

Carpena-Torres

Pintor

Lara

et al. 2020

Journal of Ophthalmology

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Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia P<0.001, corneal staining P<0.001, conjunctival hyperemia P<0.001, and levels of IL-6 mRNA P=0.005 compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia P<0.001, tear breakup time P=0.007, tear osmolarity P<0.001, density of goblet cells P<0.001, and height of mucin cloud P<0.001. Conclusions. The topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days).

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Section: Introductionmentioning

confidence: 99%

Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride

Carpena-Torres

Pintor

Lara

et al. 2020

Journal of Ophthalmology

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“…It has a protective effect on the dry eye model induced by benzalkonium chloride (BAC) and showed clinical amelioration effected by regulating the MUC1-ErbB1 pathway [9]. Buddleja officinalis Maxim Eye Drops downregulated the expressions of apoptotic factors Bax, Fas and FasL in the lacrimal gland of the dry eye rabbit model, thereby inhibiting lacrimal cell apoptosis and maintaining the basal amount of lacrimal secretion [10]. In this study, the efficacy of RMFS in treating dry eye disease was investigated.…”

Section: Discussionmentioning

confidence: 99%

Combining Runmu fengye tang preparation with hydroxysugar glycolic acid eye drops regulates Th17/Treg balance to treat dry eyes via inhibition of JAK2/STAT3 pathway

Wen,

Li,

Tian

et al. 2024

Trop. J. Pharm Res

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Purpose: To determine effective therapies for the prevention and clinical treatment of dry eye. Methods: A scopolamine-induced dry eye rabbit model was administrated with Runmu Fengliang tang (RMFS) and hypromellose 2910, dextran 70 and glycerol eye drops (HGA). Schirmer's I test (SIt), break-up time (BUT) and histopathologic examinations (H&E) were used to assess dry eye symptoms and lacrimal gland damage. Flow cytometry was used to determine the number of apoptotic cells and percentages of Th17 and Treg, while Western blot assay was used to evaluate the expression of proteins related to JAK2/STAT3 pathway. Results: The combination of RMFS with HGA significantly increased tear secretion (p < 0.001), shortened tear break-up time (p < 0.001), repaired ocular surface damage and reduced apoptosis (p < 0.05) in the scopolamine-induced dry eye model in rabbits, compared with the single-administration group. In addition, it regulated the Th17/Treg-related cytokine balance and inhibited the JAK2/SATA3 pathway. Conclusion: The combination of RMFS and HGA alleviate dry eye symptoms by inhibiting the JAK2/SATA3 pathway in model rabbits induced by scopolamine and this treatment could be a potential therapeutic strategy for the treatment of dry eye disease. Future studies should focus on clinical data collection to investigate the efficacy and safety of the combination.

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“…Among them, the total flavonoids of Buddleja officinalis play the most important role. [27][28][29] KAE, a natural flavonol found in different plant species, has been evidenced to possess effective anti-inflammatory properties. 1 With potent anti-inflammatory activities, KAE has been used to treat many acute and chronic inflammation-induced diseases, including intervertebral disc degeneration and colitis, as well as postmenopausal bone loss and acute lung injury.…”

Section: Discussionmentioning

confidence: 99%

Kaempferol Inhibits Dry Eye-Related Corneal Inflammation In Vitro Via the p38 Pathway

Huang

et al. 2022

Natural Product Communications

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ObjectiveThe purpose of this study is to investigate the effect of kaempferol (KAE), an effective constituent of Buddleja officinalis, on human corneal epithelial cells (HCECs) under a hyperosmolar environment. Methods HCECs were cultured in DMEM/F12 medium and allocated into four groups: normal, model, vehicle, and KAE. Ki67 staining was used to detect HCEC proliferation; TdT-mediated dUTP Nick-End Labeling staining was used to detect HCEC apoptosis; quantified real-time PCR was used to investigate the mRNA relative expression levels of TNF-α, IL-6, and p38. and Western blot was used to reveal the protein expression levels of TNF-α, IL-6, and p38. Results Compared with those in the normal group, the proliferation ability of HCECs was significantly inhibited and the apoptosis rate of HCECs was notably increased in the model group; but KAE could effectively improve HCEC proliferation and reduce HCEC apoptosis. Compared with those in the normal group, the mRNA relative expressions and protein levels of TNF-α, IL-6, and p38 were significantly increased in the model group, which were effectively decreased after KAE intervention. Conclusion KAE could promote HCEC proliferation and reduce cell apoptosis, as well as inhibit the expression of inflammatory factors in a hypertonic-induced HCEC model via the p38MAPK pathway.

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The Effect of Buddleja officinalis Maxim Eye Drops on Morphology and Apoptosis in Lacrimal Gland of Experimental Dry Eye Rabbit Model

Cited by 8 publications

References 10 publications

Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride

Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride

Combining Runmu fengye tang preparation with hydroxysugar glycolic acid eye drops regulates Th17/Treg balance to treat dry eyes via inhibition of JAK2/STAT3 pathway

Kaempferol Inhibits Dry Eye-Related Corneal Inflammation In Vitro Via the p38 Pathway

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