The Effect of Lactobacillus gasseri BNR17 on Postmenopausal Symptoms in Ovariectomized Rats

Abstract

Clinical and preclinical studies have reported that Lactobacillus gasseri BNR17, a probiotic bacterial strain isolated from human breast milk, reduces body weight and white adipose tissue volume. In order to further explore the actions of L. gasseri BNR17, we investigated the anti-menopausal effects of L. gasseri BNR17 in an ovariectomized (OVX) rat model. The serum alanine aminotransferase levels of the rats in the OVX-BNR17 group were lower than those of the rats in the OVX-vehicle only (OVX-Veh) group. Upon administration of L. gasseri BNR17 after ovariectomy, calcitonin and Serotonin 2A levels increased significantly, whereas serum osteocalcin levels showed a decreasing tendency. Compared to the rats in the OVX-Veh group, those in the OVX-BNR17 group showed lower urine deoxypyridinoline levels, lower pain sensitivity, and improved vaginal cornification. Furthermore, L. gasseri BNR17 administration increased bone mineral density in the rats with OVX-induced femoral bone loss. These results suggest that L. gasseri BNR17 administration could alleviate menopausal symptoms, indicating that this bacterium could be a good functional probiotic for managing the health of older women.

Anti-Melanogenic and Antioxidant Effects of Cell-Free Supernatant from Lactobacillus gasseri BNR17

“…Lactobacillus gasseri BNR17, a lactic acid bacterium, was isolated from human breast milk [15]. Our previous studies have shown that L. gasseri BNR17 has potential benefits in irritable bowel syndrome [16], weight control [17], type 2 diabetes [18], and alleviating menopausal symptoms [19]. However, no studies have assessed the anti-melanogenic and antioxidant activities of the cell-free supernatant from L. gasseri BNR17 (CFS).…”

Effect of probiotics on postmenopausal bone health: a preclinical meta-analysis

“…To further investigate the effect of probiotic treatment on bone formation, we examined the data from twenty-two selected articles that reported serum bone formation markers levels. Of these, six studies reported on serum osteocalcin (16,25,31,33,34,42) , seven studies on serum Ca (16,25,27,30,31,41,42) and six studies on serum ALP (27,29,30,34,40,41) . Pooled effect analysis was carried out using random effects model due to sufficient heterogeneity among studies.…”

“…Of these, eleven studies each were conducted on mice and rats. All the eleven studies on rats used the strain Sprague Dawley (24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34) . A total of 238 ovariectomised rats were used; 95 in control and 143 in experimental group.…”

“…A total of 215 ovariectomised mice were used, of which 88 were in control group and 127 in experimental group. Fifteen studies used Lactobacillus as the probiotic of choice (16,(24)(25)(26)(27)(28)(29)(33)(34)(35)(36)(37)(39)(40)(41) , three used Bifidobacterium (31,38,43) , and Bacillus (32) and Saccharomyces (44) were used by one study each. In one study, treatment groups included Lactobacillus, Bacillus or Bifidobacterium supplementation (30) , and in another study, treatment groups included Lactobacillus, Bifidobacterium or a mixture of Lactobacillus and Bifidobacterium (42) .…”

Ramie leaf Extract Alleviates Bone Loss in Ovariectomized Rats—The Involvement of ROS and Its Associated Signalings

“…OVX is a common method of inducing menopause in rats that reduces estrogen levels in serum. Hence, it is a prominent animal model for investigating female osteoporosis [ 24 ]. Several studies have demonstrated that a decrease in estrogen secretion increases dietary intake and white adipose tissue, resulting in metabolic obesity.…”