The Effect of Lactobacillus plantarum BW2013 on The Gut Microbiota in Mice Analyzed by 16S rRNA Amplicon Sequencing

Abstract

Lactobacillus plantarum BW2013 was isolated from the fermented Chinese cabbage. This study aimed to test the effect of this strain on the gut microbiota in BALB/c mice by 16S rRNA amplicon sequencing. The mice were randomly allocated to the control group and three treatment groups of L. plantarum BW2013 (a low-dose group of 108 CFU/ml, a medium-dose group of 109 CFU/ml, and a high-dose group of 1010 CFU/ml). The weight of mice was recorded once a week, and the fecal samples were collected for 16S rRNA amplicon sequencing after 28 days of continuous treatment. Compared with the control group, the body weight gain in the treatment groups was not significant. The 16S rRNA amplicon sequencing analysis showed that both the Chao1 and ACE indexes increased slightly in the medium-dose group compared to the control group, but the difference was not significant. Based on PCoA results, there was no significant difference in β diversity between the treatment groups. Compared to the control group, the abundance of Bacteroidetes increased in the low-dose group. The abundance of Firmicutes increased in the medium-dose group. At the genus level, the abundance of Alloprevotella increased in the low-dose group compared to the control group. The increased abundance of Ruminococcaceae and decreased abundance of Candidatus_Saccharimonas was observed in the medium-dose group. Additionally, the abundance of Bacteroides increased, and Alistipes and Candidatus_Saccharimonas decreased in the high-dose group. These results indicated that L. plantarum BW2013 could ameliorate gut microbiota composition, but its effects vary with the dose.

Lactiplantibacillus plantarum J‐15 reduced calcium oxalate kidney stones by regulating intestinal microbiota, metabolism, and inflammation in rats

“…As a probiotic, J‐15 mainly plays a role in regulating intestinal flora affected by kidney stone formation and helped restore the intestinal flora. This is pretty consistent with previous investigation on the changes in intestinal or fecal abundance of genus Lactobacillus after the administration of Lactobacillus strains in other disease models, 52,53 which might be due to the strain specificity, dosage, different animal models, and intestinal segments 54–56 . Our previous study on the microbial community composition of different intestinal segments after L .…”

“…This is pretty consistent with previous investigation on the changes in intestinal or fecal abundance of genus Lactobacillus after the administration of Lactobacillus strains in other disease models, 52,53 which might be due to the strain specificity, dosage, different animal models, and intestinal segments. [54][55][56] Our previous study on the microbial community composition of different intestinal segments after L. plantarum administration revealed that the amount of genus Lactobacillus varied widely among intestinal segments, 57 with the higher levels in the ileum and less in the cecum and colon. It suggests that L. plantarum significantly regulates the intestinal microflora of the ileum, rather than the cecum and colon.…”

Maternal supplementation with konjac glucomannan improves maternal microbiota for healthier offspring during lactation

“…66 Candidatus Saccharimonas might be associated with inflammation and the host immunological response. [67][68][69] In this research, we found that the abundance of Candidatus Saccharimonas in the KGM group was lower than in the control group. Therefore, this result could be beneficial for the health of the organism.…”

“…A reduced abundance of Clostridium is beneficial in relieving constipation 66 . Candidatus Saccharimonas might be associated with inflammation and the host immunological response 67‐69 . In this research, we found that the abundance of Candidatus Saccharimonas in the KGM group was lower than in the control group.…”

16S rRNA Gene Amplicon Sequencing of Gut Microbiota Affected by Four Probiotic Strains in Mice

“…The four probiotics used in the present study altered the structure of the gut microbiota, which is consistent with the results of other studies. Several studies have shown that L. acidophilus increased the abundance of Firmicutes and Lactobacillus and decreased the abundance of Bacteroidetes, Vibrio spp., and Ruminococcus [29]; in other studies, L. plantarum increased the abundance of Firmicutes, Proteobacteria, and Lactobacillus and decreased the abundance of Bacteroidetes, [30,31]; B. subtilis can increase the abundance of Firmicutes, Bacteroidetes, and Lactobacillus [32]; E. faecalis can increase the abundance of Bacteroidetes and Ruminococcus and decrease the abundance of Proteobacteria [33]. This is similar to the results observed in the present study, where all four probiotics affected gut microbiota diversity differently, with significant changes in abundance (p < 0.01).…”