The effect of IL-1α on the expression of matrix metalloproteinases, plasminogen activators, and their inhibitors in osteoblastic ROS 17/2.8 cells

Fujisaki, Kyosuke; Tanabe, Natsuko; Suzuki, Naoto; Mitsui, Narihiro; Oka, Hitoshi; Ito, Koichi; Maeno, Masao

doi:10.1016/j.lfs.2005.08.036

Cited by 26 publications

(23 citation statements)

References 44 publications

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“…Another study preformed by Uchida et al (2000) in mouse primary osteoblasts showed elevated mRNA levels of MMP-3,-13 after TNF-induction. Rifas et al (1994) reported that MMP-2 synthesis in normal human osteoblasts is not regulated by bone resorbing agents and in contrast to our Wndings, he reported that MMP-9 is not regulated as well (Fujisaki et al 2006;Lin et al 2003;Rifas et al 1994;Uchida et al 2000).…”

Section: Discussioncontrasting

confidence: 99%

Exposure to pro-inflammatory cytokines upregulates MMP-9 synthesis by mesenchymal stem cells-derived osteoprogenitors

David

Reznick

Srouji

et al. 2008

Histochem Cell Biol

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An intimate interplay exists between the bone and the immune system, which has been recently termed osteoimmunology. The activity of immune cells affects the intrinsic balance of bone mineralization and resorption carried out by the opposing actions of osteoblasts and osteoclasts. The aim of this study was to determine the possible interaction between inflammatory-induced conditions and matrix metalloproteinases-2,-9 (MMP-2,-9) synthesis and secretion by bone marrow-derived osteoprogenitor cells during advanced stages of osteogenesis. Rat bone marrow-derived mesenchymal stem cells (MSCs) were cultured in the presence of osteogenic supplements in order to direct the cells towards the osteogenic differentiation lineage. At the late stages of osteogenesis, assessed by histochemistry, immunohistochemistry and RT-PCR, cultures were exposed to pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 alpha (IL-1 alpha). Biochemical, histochemical and molecular biology techniques were used to discern the influence of pro-inflammatory cytokines on MMP-2,-9 synthesis and secretion. Results indicated that MMP-9 synthesis and secretion were significantly induced after exposure to the cytokines (TNF-alpha, IL-1 alpha) treatment, while MMP-2 levels remained unchanged. These results indicate that in response to inflammatory processes, osteoblasts, in addition to osteoclasts, can also be involved and contribute to the process of active bone resorption by secretion and activation of MMPs.

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Section: Discussioncontrasting

confidence: 99%

Exposure to pro-inflammatory cytokines upregulates MMP-9 synthesis by mesenchymal stem cells-derived osteoprogenitors

David

Reznick

Srouji

et al. 2008

Histochem Cell Biol

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“…Cytokines are potent autocrine regulators of granulosa or thecal cell functions (Machelon and Emilie, 1997) and are involved in the temporal and spatial remodeling of granulosa and theca cells. Further, the autocrine production of the proinflammatory cytokines was found to influence ovarian steroidogenesis (Neuvians et al, 2004;Skarzynski et al, 2005), synthesis of plasminogen activator (Hamilton et al, 1993;Fujisaki et al, 2006;Kralisch et al, 2006), synthesis of prostaglandins (Peterson et al, 1993;Neuvians et al, 2004) and synthesis of nitric oxide (Yamauchi et al, 1997;Machelon and Emilie, 1997;Estevez et al, 2004). However, the spatial expressions of cytokines in avian follicles are not studied yet.…”

Section: Introductionmentioning

confidence: 99%

Spatial expression of chemokines and cytokines mRNA in the largest preovulatory follicle of chicken

et al. 2007

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In the present experiment, we studied the spatial expression profiles of chemokines and cytokines mRNA in the granulosa (F1Gr) and theca (F1Th) layers of the largest preovulatory follicle in chicken using semi-quantitative PCR. The mRNAs of IL-1beta, IL-6, GM-CSF, chCXCLi2, chCCLi2, chCCLi4, chCCLi7, IFN-gamma, IL-4, IL-13, IL-10 and TGF-beta2 were expressed in the granulosa (F1Gr) and theca (F1Th) layers of the largest preovulatory follicle. However, the transcripts of IL-2 were not detected in any of the samples tested. Significantly higher levels of IL-6 and GM-CSF mRNA expression were noticed in F1Gr when compared to F1Th layer. Expression of chCXCLi2, a CXC chemokine, was almost similar in F1Gr and F1Th layers. However, the expression of CCL chemokines i.e. chCCLi2, chCCLi4, chCCLi7 mRNAs were almost 2 folds higher in F1Th layer in comparison to F1Gr layer. The expression of Th2 cytokines (IL-4 and IL-13) mRNA was noticed in F1Gr and F1Th layers with higher levels in the former. Expression of IFN-gamma mRNA was noticed in F1Gr and F1Th layers. Significantly higher level of TGF-beta2 expression was observed in F1Th in comparison to F1Gr layer. It was concluded from the present study that the mRNA expression of cytokines and chemokines are differentially regulated in the granulosa and theca layers of the largest preovulatory follicle in chicken.

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“…Fujisaki et al 22) reported that PA expression increases after the addition of IL-1α, whereas PAI-1 expression decreases in osteoblasts. Studies on chondrocytes have reported that IL-1…”

Section: Discussionmentioning

confidence: 99%

“…We recently found that IL-1α stimulates bone matrix turnover by increasing production of tPA and uPA and by decreasing PAI-1 production in osteoblasts 22) . We also found that nicotine promotes extracellular matrix destruction by increasing tPA expression in human osteoblastic Saos-2 cells 23) , and that nicotine and lipopolysaccharide (LPS) contribute to bone resorption by increasing tPA production in osteoblasts 24) .…”

Section: Introductionmentioning

confidence: 99%

Interleukin-17F Down-Regulates the Plasminogen/Plasmin Pathway in Chondrocytes

Tanigawa

Kawato

Aida

et al. 2011

J. Hard Tissue Biology.

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Interleukin-17 (IL-17) is a proinflammatory cytokine involved in autoimmune diseases such as rheumatoid arthritis (RA). IL-17 consists of six ligands that signal through five receptors (IL-17Rs). Serine proteinases, such as plasmin and plasminogen activators (PAs), as well as matrix metalloproteinases (MMPs) degrade the extracellular matrix during cartilage remodeling.In contrast, plasminogen activator inhibitor-1 (PAI-1), an endogenous inhibitor of PAs, forms a complex with PA to inhibit plasmin production. In the present study, we examined the effect of IL-17F on the plasminogen/plasmin pathway in cartilage using human articular chondrocytes. We evaluated the effects of IL-17F on the expression of tissue-type plasminogen activator (tPA), urokinase-type plasminogen activator (uPA), PAI-1, and cyclooxygenases (COXs), as well as on the production of prostaglandin E 2 (PGE 2 ). We also examined the indirect effects of PGE 2 on the above IL-17F-induced/ reduced components using NS-398, a specific inhibitor of COX-2 activity. Cells were cultured with or without IL-17F in the presence or absence of NS-398 for up to 28 days. mRNA and protein expression levels of PAs, PAI-1, and COXs were determined using real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. PGE 2 production was determined by ELISA. The addition of IL-17F increased the expression of PAI-1 and COX-2, as well as the production of PGE 2 , whereas uPA and COX-1 expression decreased and tPA expression was unaffected. NS-398 did not block the reducing/stimulating effects of IL-17F on the expression of COX-1 and COX-2. In contrast, NS-398 may be, in part, responsible for IL-17F-induced/reduced uPA and PAI-1 expression. Our results suggest that IL-17F suppresses the plasminogen/ plasmin pathway by increasing PAI-1 expression and decreasing uPA expression in chondrocytes.

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The effect of IL-1α on the expression of matrix metalloproteinases, plasminogen activators, and their inhibitors in osteoblastic ROS 17/2.8 cells

Cited by 26 publications

References 44 publications

Exposure to pro-inflammatory cytokines upregulates MMP-9 synthesis by mesenchymal stem cells-derived osteoprogenitors

Exposure to pro-inflammatory cytokines upregulates MMP-9 synthesis by mesenchymal stem cells-derived osteoprogenitors

Spatial expression of chemokines and cytokines mRNA in the largest preovulatory follicle of chicken

Interleukin-17F Down-Regulates the Plasminogen/Plasmin Pathway in Chondrocytes

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