The effect of immunotherapy on the state of the antioxidant system in radiation damage in rats

Abstract

Immunotropic drugs (such as sera, blood plasma and immunoglobulins) have polyfunctional activity with anti-infectious, antitoxic, homeostasis-regulating properties. Based on this, we conducted the present study with the purpose to evaluate the therapeutic effect of anti-cadmium -anti-radiation serum (AARS) on the level of radiation-chemical damage. Modeling of experimental radiation-chemical damage was carried out by preliminary 4-time subcutaneous injection of cadmium chloride solution (CdCl2) at doses of 1/500 LD50, followed by radiation exposure of 90 outbred white rats at doses of 7.0 and 9.0 Gy (LD50 and LD100, respectively). It was found that a single subcutaneous injection of TDC at a dose of 25 mg/kg of live weight 24 hours after the combined radiation-toxic damage had a radioprotective and antitoxic effect, increasing the survival rate of animals affected by lethal doses of CdCl2 and γ-rays. Increase in the survival rate of animals treated with the test agent after exposure to two-factor damage was due to inhibition of the concentration of BFR-active toxic compounds and reciprocal increase in the synthesis of metallothioneins and SOD. Thus, the use of immunotropic agent of anti-cadmium anti-radiation serum (AARS) after exposure to two-factor combined radiation-chemical damage provided 90% survival rate by induction of increased formation of metallothioneins and SOD, inhibition of redox-cycled toxic radicals formation and neutralization of cadmium toxicity with the help of anti-cadmium antibodies.