The effect of increased intakes of polyunsaturated fatty acids and vitamin E on DNA damage in human lymphocytes

Jenkinson, A. McE.; Collins, Andrew; Duthie, Susan J.; Wahle, Klaus W.J.; Duthie, G. G.

doi:10.1096/fasebj.13.15.2138

Cited by 75 publications

(34 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the absorption of these toxic compounds from the diet has been proved and confirmed in experiments with animals (Tinsley et al, 1981;Kanazawa et al, 1985;Kanazawa and Ashida, 1985;Grootveld et al, 1998;Kim et al, 1999;Jenkinson et al, 1999;Indart et al, 2002).…”

Section: Introductionmentioning

confidence: 87%

Toxic Oxygenated α,β-Unsaturated Aldehydes and their Study in Foods: A Review

Guillén¹,

Goicoechea²

2008

Critical Reviews in Food Science and Nutrition

164

115

View full text Add to dashboard Cite

The oxidation of lipids containing polyunsaturated omega-3 or omega-6 acyl groups, such as docosahexenoic, eicosapentenoic, linolenic, arachidonic, or linoleic groups, and of the corresponding fatty acids, generates among other compounds alpha,beta -unsaturated aldehydes supporting different functional groups containing oxygen, which can be named oxygenated alpha,beta -unsaturated aldehydes (OalphabetaUAs). These compounds can be produced in cells and tissues of living organisms or in foods during processing or storage, and from these latter can be absorbed through the diet. In the last few years, OalphabetaUAs are receiving a great deal of attention because they are being considered as possible causal agents of numerous diseases, such as chronic inflammation, neurodegenerative diseases, adult respiratory distress syndrome, atherogenesis, diabetes, and different types of cancer. This review deals with the nature of the different kinds of OalphabetaUAs detected until now, their reactivity and consequent biological activity; the several pathways proposed for their formation; the current knowledge about the influence of both oxidative conditions and lipids nature in the rate of formation and yield of each kind of OalphabetaUAs in edible oils; the methods described until now to determine the presence in foods of some of these compounds, such as 4-hydroxy-trans-2-nonenal, 4-hydroxy-trans-2-octenal, 4-hydroxy-trans-2-hexenal and 4-oxo-trans-2-hexenal; and finally, the levels found of some of them in several foods.

show abstract

Section: Introductionmentioning

confidence: 87%

Toxic Oxygenated α,β-Unsaturated Aldehydes and their Study in Foods: A Review

Guillén¹,

Goicoechea²

2008

Critical Reviews in Food Science and Nutrition

164

115

View full text Add to dashboard Cite

show abstract

“…Plasma was isolated by centrifugation (800 g, 10 min, 4 °C) and stored at -80 °C until analysis. Peripheral blood mononuclear cells (PBMCs) were then isolated, for the measurement of lymphocyte DNA damage, as previously described (Thies et al, 2001a) and frozen to -80 °C at a cell density of 3 × 10 6 in FCS containing 10% dimethyl sulphoxide (DMSO) at approximately -1 °C/min (Jenkinson et al, 1999).…”

Section: Blood Sampling and Preparationmentioning

confidence: 99%

Fish Oil Supplementation Reduces Markers of Oxidative Stress But Not Muscle Soreness After Eccentric Exercise

Gray¹,

Chappell²,

Jenkinson³

et al. 2014

International Journal of Sport Nutrition and Exercise Metabolism

View full text Add to dashboard Cite

Due to the potential anti-inflammatory properties of fish-derived long chain n-3 fatty acids, it has been suggested that athletes should regularly consume fish oils-although evidence in support of this recommendation is not clear. While fish oils can positively modulate immune function, it remains possible that, due to their high number of double bonds, there may be concurrent increases in lipid peroxidation. The current study aims to investigate the effect of fish oil supplementation on exercise-induced markers of oxidative stress and muscle damage. Twenty males underwent a 6-week double-blind randomized placebo-controlled supplementation trial involving two groups (fish oil or placebo). After supplementation, participants undertook 200 repetitions of eccentric knee contractions. Blood samples were taken presupplementation, postsupplementation, immediately, 24, 48, and 72 hr postexercise and muscle soreness/maximal voluntary contraction (MVC) assessed. There were no differences in creatine kinase, protein carbonyls, endogenous DNA damage, muscle soreness or MVC between groups. Plasma thiobarbituric acid reactive substances (TBARS) were lower (p < .05) at 48 and 72 hr post exercise and H2O2 stimulated DNA damage was lower (p < .05) immediately postexercise in the fish oil, compared with the control group. The current study demonstrates that fish oil supplementation reduces selected markers of oxidative stress after a single bout of eccentric exercise.

show abstract

“…It is applied that the study with cellular repair activity with DNA segments for reveal or amplify the genotoxic effects and that measuring the repair activity with DNA repair enzymes or inhibition of DNA damage by antioxidants (Jekinson et al, 1999), and quantitative measurements of specific oxidative base (Collins et al, 1993;Covallo et al, 2003).…”

Section: Resultsmentioning

confidence: 99%

Mutagenicity of octane and tetrasodium pyrophosphate in bacterial reverse mutation (Ames) test

et al. 2010

View full text Add to dashboard Cite

-We investigated the genotoxicities or mutagenicities of 2 chemicals (octane and tetrasodium pyrophosphate) with limited toxicological data in spite of their common usage based on Ames reverse mutation test. In this test, treatment of 2 chemicals at each five dose did not induce mutagenicity in Salmonella typhimurium TA98, TA100, TA1535, TA1537, and in Escherichia coli WP2uvrA with and without metabolic activation. These results indicate that 2 chemicals do not have mutagenic potentials under the conditions examined in each study. Despite these results, it can affect by inducing inhalation, skin or eye contact, ingestion, and have affected central nervous system as a target organ. It is thus necessary to prepare the local exhaust system and personal protective equipments. Based on this study, we suggest that future studies should be directed toward chronic inhalation, carcinogenic test and so on.

show abstract

The effect of increased intakes of polyunsaturated fatty acids and vitamin E on DNA damage in human lymphocytes

Cited by 75 publications

References 8 publications

Toxic Oxygenated α,β-Unsaturated Aldehydes and their Study in Foods: A Review

Toxic Oxygenated α,β-Unsaturated Aldehydes and their Study in Foods: A Review

Fish Oil Supplementation Reduces Markers of Oxidative Stress But Not Muscle Soreness After Eccentric Exercise

Mutagenicity of octane and tetrasodium pyrophosphate in bacterial reverse mutation (Ames) test

Contact Info

Product

Resources

About