The effect of indole-3-acetic-acid and benzyl amino purine concentrations on matoa (Pometia pinnata) growth in vitro

Abstract

Matoa, a Papuan fruit, is known to taste like a cross between longan, rambutan, and durian. Therefore, Matoa has seed storage and plant propagation restrictions. The tissue culture method of matoa propagation is used to solve this issue. This research aims to provide vegetative planting material that can be propagated in vitro. The study was conducted from June 2021 to January 2022 at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Sebelas Maret University, Surakarta, using a completely randomized design pattern (CRD) 2x4 treatment factors for growth regulators. There were 16 treatment combinations, which were repeated three times. The first factor is Indole Acetic Acid (IAA) concentration, which has four levels: 0 ppm, 0.5 ppm, 1 ppm, and 1.5 ppm. The second factor is Benzyl Amino Purine (BAP) concentration, which has four levels: 0 ppm, 2 ppm, 4 ppm, and 6 ppm. The results revealed that root emergence time, leaf emergence time, root number, and leaf number all had high growth rates. The number of leaves and stem nodes correlated, indicating that matoa explant growth was linked.

Effect of BAP concentration on the growth of several types of rambutan plants (Nephelium lappaceum L.) in vitro

“…Increasing the concentration of BAP causes the explants to be unable to grow roots. The addition of cytokinin in high concentrations is not necessary for the speed of root formation of matoa explants because in the study none of the explants were able to take root on media supplemented with cytokinin [19].…”

“…Endogenous cytokinin are formed in the roots, giving high concentrations of exogenous cytokinin coupled with the presence of endogenous cytokinin that have been sufficient will inhibit the growth and formation of roots [20]. The addition of BAP to matoa explants did not significantly affect the number of roots because the high concentration of cytokinin resulted in inhibited explant growth and caused explant death [19].…”