The Effect of Indonesian Velvet Bean Extract on the Fertility of Albino Male Mice

Abstract

Objective: This research aims to determine the dose of Indonesian velvet bean (Mucuna pruriens) extract which has significant effect on the ferlitity mice (concentration, motility and morphology of mice sperm). The extract is expected to become an alternative infertility herbal medicine relatively more secure and affordable replacing synthetic hormonal drugs which tend to have negative effects. Methods:The seed was taken from Yogyakarta Indonesia. Fertility test was done to fertile adult male albino mice 12 w old, weighing 25-35 grams. Fertility tests performed on seven groups of mice; i.e. negative control, positive control and treatment groups (five dose levels at 50, 100, 150, 200 and 250 mg/kg body weight). Subsequent fertility test results were statistically tested, including tests of normality (Kolmogorov-Sminov) followed by T test (Independent-Samples T Test). Results:The sperm concentration and motility increased as an increased dose of seed extract was applied, as well as decreased abnormal morphology. The highest change in the quality and quantity of sperm occurred at the dose of 250 mg/kg body weight with increased sperm concentration of about 22 million, sperm motility increased by 18% and decreased abnormal sperm morphology by 12%. Statistical analysis showed a significant increase in sperm concentration and motility at doses of 100, 150, 200 and 250 mg/kg body weight, whereas a significant decrease of abnormal sperm morphology was found at doses of 150, 200 and 250 mg/kg body weight. Conclusion: Based on this study it is concluded that IKeywords: Mucuna pruriens, L-dopa, Sperm Concentration, Sperm Motility, Sperm Morphology ndonesian velvet beans seed extract can increased the fertility of albino male mice significantly at dose level 250 mg/kg body weight.

The Different Effects of Zinc Supplement and Velvet Bean Mucuna Pruriens Extracts Toward Fertility of Balbc/ Mice

“…It indicated that higher number of sperm in A2 and A3 also gave a stronger spermatogenesis activity, whereas ZS and VBE have aphrodisiac content to increase the number of sperm. This is in line with the general knowledge that ZS can increase the number of sperm stem cells (Sedigh et al, 2016) and VBE can increase the number of sperm with stimulating the hormones of mice (Sardjono et al, 2016).…”

“…Abnormal sperm includes abnormality such as broken, detached and thin head; broken, crooked and droplet cytoplasm middle part or broke, crooked and coil tail. The observation used a microscope of 400x magnifications (Henderson and Robaire, 2005;Sardjono et al, 2016).…”

“…Infertility case has been a world issue (Sardjono et al, 2016;Martinez et al, 2012;Mathur, 2009). A review study showed a significant decrease in mean sperm count from 113 x 106 per ml in 1940 to 66 x 106 per ml in 1990 (Carlsen et al, 1992).…”

“…This infertility is consecutively caused by male (40%) and female (40%), both of female and male (10%), and another 10% of unidentified reasons. It means that the number of infertility increases in the last 50 years (Sardjono et al, 2016).…”

Prevalance of Male Infertility in India: Studies on the Effects of Gonadotropin Releasing Hormones

“…In India, the prevalence of infertility in men is increasing steadily and it has reached to such alarming levels that its incidence may affect the male population in future. The incidence cannot be ignored, therefore a detailed study involving the sperm count and hormonal profile especially FSH, LH, and testosterone concentration is an urgent necessity to ascertain the fertility problems in men [14]. Unfortunately, this study is poorly documented in India; therefore, this study can be considered as a step toward evaluating the GnRH level in infertile young male in Indian population.…”