The Effect of Inhaled Gases on Ultrasound Contrast Agent Longevity In Vivo

Itani, Malak; Mattrey, Robert F.

doi:10.1007/s11307-011-0475-5

Cited by 24 publications

(21 citation statements)

References 25 publications

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“…We posited that different vascular effects induced by these two protocols may have had an impact on the interactions between the ultrasound field, the microbubbles, and the brain microvasculature. Around the same time, two studies were published showing that the circulation time for ultrasound contrast agents was substantially reduced when subjects were breathing oxygen compared to medical air (Mullin et al 2011; Itani and Mattrey 2011), which could also explain our results.…”

Section: Introductionsupporting

confidence: 54%

“…Others have found that microbubble circulation times were reduced in animals breathing oxygen compared to medical air (Mullin et al 2011; Itani and Mattrey 2011). The results of the present study demonstrate that oxygen can have a profound diminutive effect on BBB disruption and suggests that our earlier study was not due to anesthesia, but instead to the use of oxygen.…”

Section: Discussionmentioning

confidence: 99%

“…This imbalance results in a greater driving force for microbubble dissolution. The loss of perfluorocarbon gas in the alveoli may also play a role (Itani and Mattrey 2011). The increased clearance rate was evident in the recordings of the harmonics.…”

Section: Discussionmentioning

confidence: 99%

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The Effects of Oxygen on Ultrasound-Induced Blood–Brain Barrier Disruption in Mice

McDannold

Zhang

Vykhodtseva

2017

Ultrasound in Medicine & Biology

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Numerous researchers are investigating the use of microbubble-enhanced ultrasound to disrupt the blood-brain barrier (BBB) disruption and deliver drugs to the brain. This study investigated the impact of using oxygen as a carrier gas for anesthesia on microbubble activity and BBB disruption. Targets in mice were sonicated in combination with administration of Optison microbubbles (100 μl/kg) under isoflurane anesthesia with either oxygen or medical air. A 690 kHz focused ultrasound transducer applied 10 ms bursts at peak pressure amplitudes of 0.46-0.54 MPa (N=2) or 0.34-0.36 MPa (N=5). After sonication of two locations in one hemisphere, the carrier gas for the anesthesia was changed, and the sonications were repeated in the contralateral hemisphere. The BBB disruption, measured via contrast-enhanced MRI, was significantly greater (P<0.001) with medical air than with oxygen. Harmonic emissions were also greater with air (P<0.001), while the decay rate of the harmonic emissions was 1.5 times faster with oxygen. A good correlation (R2: 0.46) was observed between the harmonic emissions strength and MRI signal enhancement. At 0.46-0.54 MPa, both the occurrence and strength of wideband emissions was greater with medical air. However, at lower peak pressure amplitudes of 0.34-0.36 MPa, the strength and probability for wideband emissions were higher with oxygen. Little or no effects were observed in histology at 0.34-0.36 MPa. These findings show that use of oxygen as a carrier gas can result in a substantial diminution of BBB disruption. These results should be taken into account when comparing studies from different researchers and in translating this method to humans.

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Section: Introductionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

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The Effects of Oxygen on Ultrasound-Induced Blood–Brain Barrier Disruption in Mice

McDannold

Zhang

Vykhodtseva

2017

Ultrasound in Medicine & Biology

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“…In such methods the quantification of bound tMBs strongly depends on the injected dose, imaging system gain, and local perfusion [137]. In addition, the influence of inhaled gases in the anaesthetic protocols influences the MBs longevity [148][149][150][151]. These studies confirmed longer circulation times of in-house lipid-shell decafluorobutane-filled UCAs and commercially available UCAs such as Definity and Albunex when animals breathe medical air instead of pure oxygen as the carrier gas for the isoflurane anaesthetic.…”

Section: Contrast-specific Imaging Techniquesmentioning

confidence: 76%

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Rooij

Daeichin

Skachkov

et al. 2015

International Journal of Hyperthermia

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Ultrasound contrast agents (UCAs) are used routinely in the clinic to enhance contrast in ultrasonography. More recently, UCAs have been functionalised by conjugating ligands to their surface to target specific biomarkers of a disease or a disease process. These targeted UCAs (tUCAs) are used for a wide range of pre-clinical applications including diagnosis, monitoring of drug treatment, and therapy. In this review, recent achievements with tUCAs in the field of molecular imaging, evaluation of therapy, drug delivery, and therapeutic applications are discussed. We present the different coating materials and aspects that have to be considered when manufacturing tUCAs. Next to tUCA design and the choice of ligands for specific biomarkers, additional techniques are discussed that are applied to improve binding of the tUCAs to their target and to quantify the strength of this bond. As imaging techniques rely on the specific behaviour of tUCAs in an ultrasound field, it is crucial to understand the characteristics of both free and adhered tUCAs. To image and quantify the adhered tUCAs, the state-of-the-art techniques used for ultrasound molecular imaging and quantification are presented. This review concludes with the potential of tUCAs for drug delivery and therapeutic applications.

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“…In one study, the decay rate on time-intensity curves measured with ultrasound imaging was almost three times higher when oxygen was used as the carrier gas for isoflurane than when medical air was used (Itani and Mattrey 2011). Another study also found a statistically significant improvement in persistence in microbubble circulation with medical air compared to oxygen as the carrier gas (Mullin et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Blood-Brain Barrier Disruption and Vascular Damage Induced by Ultrasound Bursts Combined with Microbubbles can be Influenced by Choice of Anesthesia Protocol

McDannold¹,

Zhang²,

Vykhodtseva³

2011

Ultrasound in Medicine & Biology

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Numerous animal studies have demonstrated that ultrasound bursts combined with a microbubble-based ultrasound contrast agent can temporarily disrupt the blood-brain barrier (BBB) with little or no other apparent effects to the brain. As the BBB is a primary limitation to the use of most drugs in the brain, this method could enable a noninvasive means for targeted drug delivery in the brain. This work investigated whether BBB disruption and vessel damage when overexposure occurs can be influenced by choice of anesthesia protocol, which have different vasoactive effects. Four locations were sonicated transcranially in each brain of 16 rats using an unfocused 532 kHz piston transducer. Burst sonications (10 ms bursts applied at 1 Hz for 60 s) were combined with intravenous Definity (10 μl/kg) injections. BBB disruption was evaluated using contrast-enhanced MRI. Half of the animals were anesthetized with i.p. ketamine and xylazine, and the other half with inhaled isoflurane and oxygen. Over the range of exposure levels tested, MRI contrast enhancement was significantly higher (P<0.05) for animals anesthetized with ketamine/xylazine. Furthermore, the threshold for extensive erythrocyte extravasation was lower with ketamine/xylazine. These results suggest that BBB disruption and/or vascular damage can be affected by vascular or other factors that are influenced by different anesthesia protocol. These experiments may also have been influenced by the recently reported findings that the circulation time for perfluorocarbon microbubbles is substantially reduced when oxygen is used as the carrier gas.

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The Effect of Inhaled Gases on Ultrasound Contrast Agent Longevity In Vivo

Cited by 24 publications

References 25 publications

The Effects of Oxygen on Ultrasound-Induced Blood–Brain Barrier Disruption in Mice

The Effects of Oxygen on Ultrasound-Induced Blood–Brain Barrier Disruption in Mice

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Blood-Brain Barrier Disruption and Vascular Damage Induced by Ultrasound Bursts Combined with Microbubbles can be Influenced by Choice of Anesthesia Protocol

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